Aged-related changes of brain derived neurotrophic factor and γ-aminobutyric acid expression in the visual cortex of rat

doi:10.16098/j.issn.0529-1356.2015.02.002

Abstract

Abstract:

Objective To compare the expression changes of brain-derived neurotrophic factor (BDNF) and inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the primary visual cortex between different age groups of rats, and to provide clues for exploring the cellular molecular mechanisms that underlie senescence-related visual function declines.Methods Nissl staining was used for identification of visual cortical layers and cell counting. Immunohistochemical methods were employed to display the BDNF and GABA-immunopositive neurons in the primary visual cortex of rats. Sections were observed under a light microscope, and the cell density and the optical absorbance intensity of immunoreactions were measured and analyzed with Image-Pro Express 6.0 software. Results The mean density of Nissl-stained neurons in each cortical layer of V1 showed no significant difference among young(n=6) ,middle-aged(n=6) and elderly groups (n=6). Compared to the young group of rats, the average density and immunoreactive intensity of BDNF and GABA-positive neurons in each cortical layer of V1 in middle-aged and elderly groups were significantly decreased. The average density and intensity of BDNF and GABA-immunoreactive neurons in each cortical layer of V1 in the elderly group were significantly lower than those in the middle-aged group. The reduction of GABA expression in each cortical layer of V1 was highly correlated to the decrease of BDNF expression during the aging process.Conclusion The expression of BDNF and GABA in the primary visual cortex showed a progressive decline during aging process. The reduction of BDNF secretion may result in a down-regulation of GABA expression in the brain ,which may be one of the important mechanisms that mediate the visual function declines during aging.

Key words: Visual cortex, Brain derived neurotrophic factor, γ-Aminobutyric acid;, Age, Immunohistochemistry, Rat

WANG Zi-lu JIANG San JIN Cai-hong YANG Jin-fang WANG Qian HUA Tian-miao*. Aged-related changes of brain derived neurotrophic factor and γ-aminobutyric acid expression in the visual cortex of rat[J]. AAS, 2015, 46(2): 151-157.

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