Expression of oligodendrocyte transcription factor and neurological cadherin in the developing spinal cord of the human embryo

Abstract

Abstract:

Objective To investigate distribution of the oligodendrocyte transcription factor (Olig) and neurological cadherin（N-cadherin） in the developing human embryonic spinal cord and their clinical significance. Methods Immunohistochemistry was uesd to detect the expression and distribution of Olig and N-cadherin proteins in the spinal cord of 17 human embryos at the second, third or fourth month gestation. One-Way ANOVA and LSD-t test were employed to compare positive expression number and intensity values of Olig and N-cadherin proteins in spinal cord tissues detected in the 3 fetal periods. Results At the second to fourth gestation, the positive expressions of Olig and N-cadherin were seen in the human embryonic spinal cord. With the increase of gestational age, the intensity values of N-cadherin positive cells were gradually increased in the spinal cord, and positive expression intensity values were 117.77±6.72，129.63±9.44 and 133.35±8.15(P<0.01), respectively. Olig positive expression intensity values showed an up and then down trend were 69.98±24.59，85.10±9.19 and 71.62±11.81(P<0.05), respectively. The number of Olig positive cells in the spinal cord were gradually increased and were 9.38±7.80，15.55±7.30，25.59±14.54 (P<0.01), respectively. The results of Olig and N-cadherin showed a significant difference in the spinal cord. Conclusion At the second to fourth gestation, the positive expressions of N-cadherin and Olig suggest that they are closely related to the nerve and glial cells differentiation and development in the spinal cord tissues during the human embryonic growth and development.

Key words:

Embryo, Spinal cord, Oligodendrocyte transcription factor, Neurological cadherin, Immunohistochemistry, Human

ZHANG Yong ZHANG Ju-shi CHEN Jian-er LIU Xue-hong*. Expression of oligodendrocyte transcription factor and neurological cadherin in the developing spinal cord of the human embryo[J]. AAS, 2015, 46(5): 655-659.

References

［1］Vue TY, Kim EJ, Parras CM, et al. Ascl1 controls the number and distribution of astrocytes and oligodendrocytes in the gray matter and white matter of the spinal cord［J］. Development, 2014, 141(19):3721-3731.
［2］Rodriguez JP, Coulter M, Miotke J, et al. Abrogation of β-catenin signaling in oligodendrocyte precursor cells reduces glial scarring and promotes axon regeneration after CNS injury［J］. J Neurosci, 2014, 34(31):10285-10297.
［3］Wrzos C, Winkler A, Metz I, et al. Early loss of oligodendrocytes in human and experimental neuromyelitis optica lesions［J］. Acta Neuropathol, 2014,127(4):523-538.
［4］Malinverno M, Carta M, Epis R, et al. Synaptic localization and activity of ADAM10 regulate excitatory synapses through N-cadherin cleavage［J］. J Neurosci, 2010,30(48):16343-16355.
［5］Lelièvre EC, Plestant C, Boscher C, et al. N-cadherin mediates neuronal cell survival through Bim down-regulation［J］.PLoS One, 2012，7(3):e33206.
［6］Sakai N, Insolera R, Sillitoe RV, et al. Axon sorting within the spinal cord marginal zone via Robo-mediated inhibition of N-cadherin controls spinocerebellar tract formation［J］. J Neurosci, 2012, 32(44):15377-15387.
［7］Demireva EY, Shapiro LS, Jessell TM, et al. Motor neuron position and topographic order imposed by β-and γ-catenin activities［J］. Cell, 2011, 147(3):641-652.
［8］Kuroiwa M, Watanabe M, Katoh H, et al. Effect of amiloride on endoplasmic reticulum stress response in the injured spinal cord of rats［J］. Eur J Neurosci, 2014,40(7):3120-3127.
［9］Fu SL, Yang CQ, Jia YY, et al. The effect of N-Cadherin misexpression on neuron migration during mouse cerebral cortex development［J］. Chinese Journal of Immunology, 2013, 29(8):866-869, 871.(in Chinese)
付苏雷，杨慈清，贾阳阳，等. 小鼠胚胎发育过程中N-Cadherin异常表达对大脑皮层神经元迁移的影响［J］. 中国免疫学杂志，2013，29（8）：866-869，871.
［10］Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis［J］. Development, 1988,102(4):639-655.
［11］García-Castro MI, Vielmetter E, Bronner-Fraser M. N-Cadherin, a cell adhesion molecule involved in establishment of embryonic left-right asymmetry［J］. Science, 2000, 288(5468):1047-1051.
［12］Shih W, Yamada S. N-cadherin as a key regulator of collective cell migration in a 3D environment［J］. Cell Adh Migr, 2012, 6(6):513-517.
［13］Yang CQ，Du R， Lin JT，et al. Co-expression patterns of N-cadherin and neurofilament in spinal cord during chicken embryonic development［J］. Chinese Journal of Immunology, 2013, 29(3):232-235. (in Chinese)
杨慈清，杜蕊，林俊堂，等. 鸡胚发育过程N-Cadherin和Neurofilament在脊髓中共表达模式的研究［J］. 中国免疫学杂志，2013，9（3）：232-235.
［14］He L, Lu QR. Coordinated control of oligodendrocyte development by extrinsic and intrinsic signaling cues［J］. Neurosci Bull, 2013, 29(2):129-143.
［15］Yao HB, Zhang M, Gong XW, et al. Effects of remyelination of Olig on oligdendrcyte mediated demyelination in the rat［J］. Acta Anatomica Sinica, 2014,45(1):37-40. (in Chinese)
姚宏波，张萌，宫学武，等. Olig对少突胶质细胞介导脱髓鞘大鼠髓鞘再生的影响［J］.解剖学报，2014，45（1）：37-40.

[1]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[2]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[3]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[4]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[5]	YAN Kun WU Xiao-lin LIU Ying-juan GE Ke-li1 REN Lei-ming LI Hong-yun . Effect of cerebrotein hydrolysate-Ⅰon intestinal microflora regulation of mice with Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(5): 497-504.
[6]	ZHUANG Hai-rong WU Zi-dong CHEN Xue-hong LI Cheng-jun. Repairing effect of sulodexide on diabetic retinopathy and the regulation of MAPK pathway in rats [J]. Acta Anatomica Sinica, 2023, 54(4): 392-399.
[7]	YU Hua-jing WEI Lu-yang LIU Shan-shan GUAN Cheng-jian ZHANG Zhong-tao. Role and clinical significance of MLLT1 super elongation complex subunit in the occurrence and development of hepatocellular carcinoma [J]. Acta Anatomica Sinica, 2023, 54(4): 425-433.
[8]	WANG Xiao LI Liang CUI Cheng-li CAI Zhi-ping YU Chang ZHANG Li-jia . Endurance exercise combined with intermittent cold stimulation promoting white adipose tissue browning in rats [J]. Acta Anatomica Sinica, 2023, 54(3): 348-356.
[9]	ZHANG Yi-zhou LI Sha MI Shi-xiong ZUO Hong-chun ZHANG Qian-qian LI Han-lin LEI Zi-han ZHANG Dong-ze CUI Hui-xian. Effect of estradiol regulating sortilin-related receptor A expression on hippocampal spine density and synaptic protein expression via estrogen receptor of mouse [J]. Acta Anatomica Sinica, 2023, 54(3): 261-268.
[10]	LIU Ke-ting CHEN Bin-bin SHUI Yu-lian SHI Qing-ming XIAO Li. Neuroprotective effect of exogenous hydrogen sulfide and the expression of matrix metalloproteinases-9 and Caspase-1 in mice model of Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(3): 289-295.
[11]	LIU Xia ZHENG Li-ping DU Xiao-hua WANG Yu-jiao WU Ya-juan . Expression and localization of neuroglobin in the brain tissue of Saiga antelope [J]. Acta Anatomica Sinica, 2023, 54(2): 188-194.
[12]	HU Xiao-jing ZHANG Ming-min Lü Guang-ming. Preliminary analysis of mRNA and microRNA differential expression profiles in spinal cord regeneration of Gekko japonicus after tail amputation [J]. Acta Anatomica Sinica, 2023, 54(1): 6-12.
[13]	LI Qiao LIU Xia DU Xiao-hua MI Xiao-yu WEN Yong-qiang FANG Ying-dong . Expression and localization of vascular endothelial growth factor-B in diencephalon and brainstem related tissues of the Yak’s brain [J]. Acta Anatomica Sinica, 2023, 54(1): 30-35.
[14]	SU Xiao-yun HE Ji-ping CUI Jian-mei SHI Xian-feng MEN Jie. Effects of estradiol on depressive-like behavior of ovariectomized rats and its possible mechanism [J]. Acta Anatomica Sinica, 2023, 54(1): 36-41.
[15]	GAO Sai-hong ZHANG Xiao-liang YANG Ying-chun QIAO Hai-bing. Activation of p38 MAPK and its effect on the expression of Bax and Bcl-2 after cerebral ischemia-reperfusion in hyperlipidemia rats [J]. Acta Anatomica Sinica, 2023, 54(1): 50-55.