Clinical significance of phosphorylated signal transducer and activator of transcription 3 activing and interleukin-17 in patients with breast cancer

doi:10.16098/j.issn.0529-1356.2015.06.011

Abstract

Abstract:

Objective To investigate involvement of constitutively activated phosphorylated signal transducer and activator of transcription 3(p-STAT3) and the expression of its downstream inflammatory cytokine interleukin (IL)-17 in the pathogenesis and progression in patients with breast cancer(BC). Methods Using specific antibodies, the levels of IL-17 and p-STAT3 were tested in 379 cases of breast cancer tissues (test group) and 245 cases of matching non-cancerous adjacent breast adinosis tissues (control group) using immunohistochemistry (IHC). The correlations of expressed IL-17 and active p-STAT3 were analyzed against breast cancer patients’ histological type, TNM and clinical stage, and progression, respectively. Quantitative data were analyzed using t test and qualitative data were analyzed using χ2 test. Results 1. IL-17 showed a positive expression rate of 95.8% in brest cancer tissues, higher than that of 85.3% in adenosis of breast tissues (χ2=21.36, P＜0.001); p-STAT3 exhibited a positive expression rate of 93.6% in BC tissues, much higher than that of 62.0% in adenosis of breast tissues (χ2=97.7，P<0.001). 2. The expressed IL-17 and active p-STAT3 were not correlated with histologic types of breast cancer (including invasive ductual carcinoma, invasive lobulao carcinoma, ductual carcinoma in situof breast) (χ2=1.245, P=0.535). 3. However, IL-17 and p-STAT3 were respectively correlated with lymph node metastasis in patients with BC (χ2=7.806, P<0.01; χ2=4.053, P<0.05, respectively). 4. In breast cancer tissues, the expression of IL-17 was positively correlated with activated p-STAT ( rs=0.136, P<0.01). Conclusion The constitutively over-active p-STAT3 and over-expressed IL-17 may collaborate to orchestrate an inflammatory microenvironment which, in turn, facilitates the pathogenesis and progression of breast cancer.

Key words: Interleukin-17, Phosphorylated signal transducer and activator of transcription 3, Breast cancer, Metastasis, Immunohistochemistry, Human

WANG Ju ZHANG Nan MA Zhi-ping LI Yi-xing ZHANG Wen-jie* ZHOU Zong-yao*. Clinical significance of phosphorylated signal transducer and activator of transcription 3 activing and interleukin-17 in patients with breast cancer[J]. AAS, 2015, 46(6): 791-796.

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