Ultrastructural features of enteric glial cells and the distribution of S-100 beta in the small intestine of chicken

Abstract

Abstract: objective To observe the structure of Enteric glial cell and distribution of S-100β protein in 10 healthy yellow feather broiler chickens. Methods The ultrastructural characteristics of glial cells were observed by transmission electron microscopy， and the distribution of S-100 protein was studied by immunohistochemical SABC-AP method. Results The electron microscope showed that chicken enteric glial cells (EGCS) in the small intestine were star， irregular nucleus， cytoplasm distribution has a small round or oval unmyelinated nerve fibers. Immunohistochemistry showed that in chicken intestine epithelium mucosa of each segment， intestinal glands of S-100βwere expressed strong. Among the basement membrane of epithelial cells and at the top of intestinal gland epithelial cells were the strong positive， lamina propria weaker expression， in the submucosal plexus and myenteric plexus showed strong positive expression. Conclusion the glial cell ultrastructure characteristics and distribution characteristics of S-100βare similar to the small intestine of mammals.

Key words: Enteric glial cell, Ultrastructural, S-100β, the small intestine, immunohistochemistry, chicken

References

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