Regulatory effects of sericin on pancreatic insulin PI3K/Akt signaling pathway in type 2 diabetic rats

doi:10.16098/j.issn.0529-1356.2016.06.015

Abstract

Abstract:

Objective To investigate the effects of sericin on the blood glucose concentration by regulating pancreatic insulin P13K/Akt signaling pathway. Methods Thirty-six male SD rats were randomly divided into normal control group, diabetes mellitus model group and sericin treatment group, with 12 rats in each group. The type 2 diabetes mellitus rats model was induced by high calorie and high sugar diet plus intraperitoneal injection of streptozotocin (35mg/kg, 2 times, 1 time a day), and the standard for successfully establishing type 2 diabetes model was fasting blood glucose≥11.1mmol/L. The model rats were treated with sericin at the dose of 2.4g/kg/d for 35days by gastrogavage method. ELISA was used to detect the adiponectin level in serum, Western blotting and real time fluorescence quantitative PCR to detect the expression of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt/PKB) protein and mRNA in pancreatic tissues. Results Compared with model group, the serum adiponectin level, IR, IRS-1, PI3K and Akt/PKB protein and mRNA expression in pancreatic tissues of rats in sericin treatment group increased markedly (P＜0.01,P＜0.05). Conclusion Sericin can improve the abnormal changes of PI3K/Akt signaling pathway by up regulating IR, IRS-1, PI3K and Akt expression in pancreas of diabetes mellitus rats; Thereby, sericin play a role in lowering blood glucose.

Key words: Sericin, Type 2 diabetes mellitus, Pancreas, Insulin PI3K/Akt signaling pathway, ELISA, Rat

LIU Mei-xiao SUN Yi-chan HOU Li-na YANG Song-he CHENG Lu-yang SONG Cheng-jun CHEN Zhi-hong. Regulatory effects of sericin on pancreatic insulin PI3K/Akt signaling pathway in type 2 diabetic rats[J]. Acta Anatomica Sinica, 2016, 47(6): 807-811.

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