Inhibition effect of sorafenib on adjuvant arthritis in rats

doi:10.16098/j.issn.0529-1356.2017.02.012

Abstract

Abstract:

Objective To observe the inhibition effect of sorafenib on adjuvant arthritis in rats. Methods A total of 36 male SPF SD rats were divided into 6 groups and 0.1ml of the complete Freund’s adjuvant was subcutaneously injected into the left hind paw except the normal group. The volume of rat hind paw was measured. The CD4⁺ and CD8⁺T cell subsets of the peripheral blood were analyzed by flow cytometry. The microvessel density of synovial tissues was determined by immunohistochemical chain mildew avidin peroxidase enzymatic (SP) method. Results Compared with model group, the rats of sorafenib groups alleviated the volume of rat hind paw and reduced microvessel density. Sorafenib (20, 40mg/kg) groups decreased the percentage of CD4⁺T cell and increased the percentage of CD8⁺T cell at the same time. All the values were statistically significant(P<0.05). Conclusion Sorafenib can ameliorate adjuvant arthritis in rats, which effect may be related to sorafenib causing CD4+ and CD8+T cell subset of peripheral blood deviation and reducing microvessel density of synovial tissues.

Key words: Adjuvant arthritis, Sorafenib, T cell subset, Microvessel density, Immunohistochemical SP method, Flow cytometry, Rat

LIU Fei GONG Yong-fang WANG Zhen-zhen ZHANG Xiao-ming DENG Xue-fei JIAO Yi HUANG Xue-ying. Inhibition effect of sorafenib on adjuvant arthritis in rats[J]. Acta Anatomica Sinica, 2017, 48(2): 187-192.

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