Effect of transferrin receptor 1 on the growth and differentiation of mouse hippocampal neurons

doi:10.16098/j.issn.0529-1356.2017.04.005

Abstract

Abstract:

Objective To investigate the effect of transferrin receptor 1 (TfR1) on the growth and differentiation of mouse hippocampal neurons. Methods Hippocampal neurons were isolated from C57BL/6 mouse at embryonic day 18.5, and then transfected by TfR1 shRNA pGFP-V-RS plasmid after 7 days culture. The transfection efficiency of TfR1 shRNA was detected by green fluorescent protein(GFP) and Western blotting ting assay. 4'-6-diamidino-2-phenylindole(DAPI) and TUNEL stainings were used to detect the apoptosis of hippocampal neurons. Immunofluorescence staining was used to analyze the changes of neuronal cytoskeleton and the morphological characteristics of neurite growth. Results TfR1 shRNA interference plasmid effectively knockdowned TfR1 gene in cultured neurons. TfR1 shRNA interference obviously induced neurons apoptosis and the disintegration of cytoskeletons. Accordingly, the neurite elongation significantly increased after TfR1 gene interfered (P<0.05). Conclusion TfR1 can affect the differentiation of hippocampal neurons by regulating the neurite outgrowth of mouse hippocampal neurons.

Key words: Transferrin receptor 1, Dendrite, Western blotting, Mouse

WANG Feng-gang FAN Wen-juan. Effect of transferrin receptor 1 on the growth and differentiation of mouse hippocampal neurons [J]. Acta Anatomica Sinica, 2017, 48(4): 397-403.

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