Effects of resveratrol on Shh signaling pathway of NIH3T3 cells

doi:10.16098/j.issn.0529-1356.2018.02.007

Abstract

Abstract:

Objective To investigate the effect of resveratrol on Shh signaling pathway of NIH3T3 cells in vitro. Methods NIH3T3 cells were divided into the control and resveratrol groups. The cells were cultured with resveratrol for 24 hours. Cell viability was detected with CCK-8 assay．Immunofluorescence measured the expressions of Ac-tu, Smo, and Gli-1. Western blotting assayed the expressions of Shh, Ptc-1, Smo and Gli-1 proteins. Results Compared with the control group（0.585±0.039）, 0.5（0.679±0.047, P＜0.05 and 1.0 μmol/L（0.774±0.054, P＜0.05 resveratrol significantly enhanced the viability of NIH3T3 cells, and the peak was 1.0 μmol/L. On the contrary, 10, 20, 40, 80 μmol/L resveratrol significantly decreased the viability of NIH3T3 cells（0.428±0.043, 0.395±0.031, 0.373±0.017, 0.361±0.016, respectively). However, 0.1（0.602±0.065）and 5 μmol/L（0.556±0.041）resveratrol did not affect the viability（P>0.05）. NIH3T3 cells had one primary cilia and expressed the proteins of Ac-Tu, Shh, Ptc-1, Smo and Gli-1.Smo and Gli-1 proteins were located in the cytoplasm. At 24 hours for resveratrol treat, Gli-1 protein translocated into the nucleus from cytoplasm and Smo protein entered the primary cilia from the cytoplasm. Expressions of Shh（0.756±0.659 vs 0.441±0.769，P＜0.05,Ptc-1（0.655±0.347 vs 0.351±0.026，P＜0.05,Smo（0.779±0.064 vs 0.451±0.035，P＜0.05 and Gli-1（0.856±0.044 vs 0.560±0.058，P＜0.05 proteins significantly compared with the control group. Conclusion Resveratrol can enhance viability of NIH3T3 cells via activating Shh signaling pathway.

Key words: Resveratrol, Shh signaling pathway, Primary cilia, NIH3T3 cells, Western blotting

GUO Shuang LIAO Hong-yan LIU Jie TANG Fan-ren YANG Qin. Effects of resveratrol on Shh signaling pathway of NIH3T3 cells[J]. Acta Anatomica Sinica, 2018, 49(2): 179-184.

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