Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells

doi:10.16098/j.issn.0529-1356.2018.02.011

Abstract

Abstract:

Objective To explore the roles and mechanisms of silence trefoil factor 3(TFF3) on migration, invasion,clony formation and epithelial-mesenchymal transition of thyroid papillary carcinoma TPC-1 cells. Methods Expressions of Snail and TFF3 in tissue microarrays of thyroid papillary carcinoma were analysed by immunohistochemistry. Invasion assays, wound healing assays and colony formation tests were performed to detect the effects of TFF3 on migration, invasion and clony formation of TPC-1 cells. Epithelial-mesenchymal transition(EMT) markers and regulatory molecules were detected by Real-time PCR assay,Western blotting and immunocytochemistry in TPC-1 cells and mitogen-activated protein kinase (MAPK) signalling pathway related proteins were examined by Western blotting and immunocytochemistry. Results In 31 cases of papillary thyroid carcinoma, Snail and TFF3 positive signals were observed in the cytoplasm of papillary throid carcinoma(PTC),and were negative in the para-carcinoma.The expression of Snail in papillary thyroid carcinoma showed positive correlation with the expression of TFF3 protein(r=0.8450,P<0.05). Siliencing TFF3 expression markedly inhibited the ability of migration，invasion and clony formation in vitrovia suppressing of the MAPK signalling pathway and inhibited the process of EMT(P<0.05 or P<0.01). Conclusion Knockdown of TFF3 inhibits TPC-1 cells migration,invasion clony formation and EMT related proteins epression through suppressing MAPK/ERK signalling pathway.

Key words: Trefoil factor 3, Epithelial-mesenchymal transition, Immunohistochemistry, Western blotting, Real-time PCR, Human

LIN Xu WANG Zhi-yong WU Jing-fang ZHANG Wen-jing ZHANG Jing XUE Gang. Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells[J]. Acta Anatomica Sinica, 2018, 49(2): 204-211.

References

［1］Meng L, Hu S, Huang L, et al.Papillary thyroid cancer coexisting with thyroid tuberculosis: a case report［J］. Oncol Lett, 2014, 7(5):1563-1565.

［2］Yoon SG, Yi JW, Seong CY, et al.Clinical characteristics of papillary thyroid carcinoma arising from the pyramidal lobe［J］. Ann Surg Treat Res, 2017, 92(3):123-128.

［3］Illam SP, Narayanankutty A, Mathew SE, et al. Epithelial mesenchymal transition in cancer progression: preventive Phytochemicals［J］. Recent Pat Anticancer Drug Discov, 2017, 12(3):234-246.

［4］May FE, Westley BR.TFF3 is a valuable predictive biomarker of endocrine response in metastatic breast cancer［J］. Endocr Relat Cancer, 2015, 22(3):465-479.

［5］Li Y, Sun Z, Liu K, et al.Prognostic significance of the co-expression of nucleophosmin and trefoil factor 3 in postoperative gastric cancer patients［J］. Mol Clin Oncol, 2014, 2(6):1055-1061.

［6］Xiao L, Liu YP, Xiao CX, et al.Serum TFF3 may be a pharamcodynamic marker of responses to chemotherapy in gastrointestinal cancers［J］. BMC Clin Pathol, 2014, 14(1):26.

［7］Zhang XJ,Wu JF,Huang J,et al. Expression and clinical significance of trefoil factor 3 in patiens with papillary thyroid carcinoma［J］.Acta Anatomica Sinica, 2013(4):503-508. (in Chinese)

张小俊, 吴靖芳, 黄静, 等.三叶因子3在甲状腺乳头状癌中的表达及临床意义［J］. 解剖学报, 2013(4):503-508.

［8］Wu JF. Study on the effect and mechanism of RNA inference targeting TFF3 gene on the proliferation and invasion ability of papillary carcinoma cells［D］. Hebei Medical University, 2016. (in Chinese)

吴靖芳. RNA干扰TFF3基因对人甲状腺乳头状癌细胞增殖与侵袭的影响及机制研究［D］. 河北医科大学, 2016.

［9］You ML, Chen YJ, Chong QY, et al.Trefoil factor 3 mediation of oncogenicity and chemoresistance in hepatocellular carcinoma is AKT-BCL-2 dependent［J］. Oncotarget, 2017, 8(24):39323-39344.

［10］Khoury T, Chadha K, Javle M, et al.Expression of intestinal trefoil factor (TFF-3) in hepatocellular carcinoma［J］. Int J Gastrointest Cancer, 2005, 35(3):171-177.

［11］Pandey V, Wu ZS, Zhang M, et al.Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma［J］. Breast Cancer Res, 2014, 16(5):429.

［12］Yuan Z, Chen D, Chen X, et al.Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells［J］. Cancer Cell Int, 2017, 17(1):7.

［13］Hoffmann W.Trefoil factors TFF (trefoil factor family) peptide-triggered signals promoting mucosal restitution［J］. Cell Mol Life Sci, 2005, 62(24):2932-2938.

［14］Kannan N, Kang J, Kong X, et al.Trefoil factor 3 is oncogenic and mediates antiestrogen resistance in human mammary carcinoma［J］. Neoplasia, 2010, 12(12):1041-1053.

［15］Lamouille S, Xu J, Derynck R.Molecular mechanisms of epithelial-mesenchymal transition［J］. Nat Rev Mol Cell Biol, 2014, 15(3):178-196.

［16］Romano S, Staibano S, Greco A, et al.FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential［J］. Cell Death Dis, 2013, 4 (4):e578.

［17］Saxena M, Stephens MA, Pathak H, et al.Transcription factors that mediate epithelial-mesenchymal transition lead to multidrug resistance by upregulating ABC transporters［J］. Cell Death Dis, 2011, 2 (7):e179.

［18］Zhao XL, Sun T, Che N, et al.Promotion of hepatocellular carcinoma metastasis through matrix metalloproteinase activation by epithelial-mesenchymal transition regulator Twist1［J］. J Cell Mol Med, 2011, 15(3):691-700.

［19］Li S, Lu J, Chen Y, et al.MCP-1-induced ERK/GSK-3beta/Snail signaling facilitates the epithelial-mesenchymal transition and promotes the migration of MCF-7 human breast carcinoma cells［J］. Cell Mol Immunol, 2017, 14(7):621-630.

［20］Zhou Q, Chen J, Feng J, et al. SOSTDC1 inhibits follicular thyroid cancer cell proliferation, migration, and EMT via suppressing PI3K/Akt and MAPK/Erk signaling pathways［J］. Mol Cell Biochem, 2017, 435 (1-2):87-95.

［21] Baus-Loncar M, Giraud A. SMultiple regulatory pathways for trefoil factor (TFF) genes［J］. Cell Mol Life Sci, 2005, 62(24):2921-2931.

［22］Lin J, Sun Z, Zhang W, et al.Protective effects of intestinal trefoil factor (ITF) on gastric mucosal epithelium through activation of extracellular signal-regulated kinase 1/2 (ERK1/2)［J］. Mol Cell Biochem, 2015, 404(1-2):263-270.

［23］Xing M.Molecular pathogenesis and mechanisms of thyroid cancer［J］. Nat Rev Cancer, 2013, 13(3):184-199.

[1]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[2]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[3]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[4]	LIU Xiao-li XU Lin WU Ai-xue WEN Cai-yun LI Ru-hua WU An-ting CHEN Dai-qian CHEN Cheng-chun. Analysis of cerebral gray matter structure in multiple sclerosis and neuromyelitis optica [J]. Acta Anatomica Sinica, 2024, 55(1): 17-24.
[5]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[6]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[7]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[8]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[9]	BAI He TENG Ye FENG Lei MENG Hai-wei1 TANG Yu-chun LIU Shu-wei. 3D hippocampal segmentation based on spatial and frequency domain features adaptive fusion and inter-class boundary region enhancement [J]. Acta Anatomica Sinica, 2024, 55(1): 73-81.
[10]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[11]	YIN Shi-qin YANG Si-yi WANG Rui-han YOU Gui-xuan YANG Ying-qiu ZHANG Lei. Distal tibiofibular syndesmosis fibular notch typing and its clinical significance based on CT [J]. Acta Anatomica Sinica, 2024, 55(1): 82-87.
[12]	YANG Yang SHI Jun LI Kun MA Yuan ZHANG Shao-jie HOU Er-fei WANG Chao-qun CHEN Jie WANG Xing LI Zhi-jun. Finite element analysis of cervical intervertebral discs after removing different ranges of uncinate processes [J]. Acta Anatomica Sinica, 2024, 55(1): 88-97.
[13]	SUN Lei WANG Xing-yu XIE Shui-hua. Risk analysis of re-fracture after percutaneous kyphoplasty in elderly patients with osteoporotic thoracolumbar compression fractures and construction of acolumnar graph prediction model#br# [J]. Acta Anatomica Sinica, 2024, 55(1): 98-104.
[14]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[15]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.