Abstract:

Objective The purpose of this study is to evaluate the effect of oophorus cumulus granulosa cells bone morphogenetic protein(BMP)-15，BMP-6 signaling molecules expression on blastocyst information after intractoplasmic sperm injection(ICSI), and assess the associations with the developmental ability of oocytes through ICSI. Methods A total of 119 women receiving ICSI due to male infertility in Fujian Maternity and Children Health Hospital during the period from April 2012 to June 2014 were enrolled in the study.456 oophorus cumulus granulosa cell samples were collected from each oocyte for ICSI. BMP-15, BMP-6, AKL2, AKL6, BMPRⅡ, Smad1, Samd4, Smad5, Samd8 and zinc finger E-box-binding protein(ZEB1) signaling molecules were determined by immunocytochemistry staining and Western blotting assay, while the mRNA expression was quantified using Real-time PCR assay. The molecules expression differences between blastocyst and non-blastocyst groups after ICSI were compared. Results The intensities of immunohistochemical positive staining for BMP15, BMP-6,AKL2,AKL6, BMPRⅡ, Smad1,2,5,and 8 and ZEB1 were at various levels and the positive staining were distributed in oophorus cumulus cells. The intensities of immunohistochemical staining for BMP-15,BMP-6, AKL2,6, BMPRⅡ,Smad1,4 and ZEB1 were higher in the blstocyst group than those in non-blastocyst group from ICSI (P＞0.05). While there no significant differences for staining intensities of Smad5, Smad8 between two groups (P＞0.05). While there was no significant differences of Smad5, Samd8 expression were found between two groups (P＞0.05). Conclusion BMP-15, BMP-6, AKL2, AKL6, BMPRⅡ, Smad1, Samd4 and ZEB1 signaling molecules may play an important role in the development of oocytes and promotion of blastocyst information from embryo culture after oocyte fertilization through ICSI.

Key words: Blastocyst, Cumulus granulosa cell, Bone morphogenetic protein, Intractoplasmic sperm injection, Female