MiR-382 promoting BRL-3A cell proliferation of rat

doi:10.16098/j.issn.0529-1356.2018.04.004

Abstract

Abstract:

Objective To explore the effect of miR-382 on cell proliferation and apoptosis of rat hepatocyte line BRL-3A in vitro. Methods BRL-3A cells were transiently transfected with miR-382 mimics and inhibitors for 48 hours，MTT assay was used to detect the cell viability. Flow cytometry was used to observe the cell cycle. The expression of proliferation/apoptosis-related genes was detected by Real-time PCR. Results The expression of miR-382 was significantly up-regulated by treating with miR-382 mimics in the rat BRL-3A cells，and the expression of miR-382 was significantly down-regulated by treating with miR-382 inhibitor in the rat BRL-3A cells(P<0.01). MTT result showed that the activity of BRL-3A cells was significantly increased after miR-382 overexpression. Flow cytometry showed that the number of cells in S phase of miR-382 overexpression group was significantly higher than that of the negative control group, while the number of cells in S phase of miR-382 interference group was significantly lower than that of the negative control group. The mRNA expression levels of poliferating cell nuclear antigen(PCNA), Bcl-2 and Ccnd1 in BRL-3A cells was up-regulated and the mRNA expression levels of Caspase-3 and Bax were down-regulated by Real-time PCR after miR-382 overexpression, while the miR-382 interference group was opposite to the above result. Conclusion sMiR-382 may promote cell proliferation via regulating the expression of proliferation-related apoptosis-related proteins in rat hepatocyte line BRL-3A.

Key words: MiR-382, BRL-3A cell, Cell cycle, Cell proliferation, Real-time PCR, Rat

GAO Hang ZHANG Chun-yan WHANG Feng-juan XU Cun-shuan. MiR-382 promoting BRL-3A cell proliferation of rat[J]. Acta Anatomica Sinica, 2018, 49(4): 437-442.

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