Effects of heroin on oxidative damage and specific protein expression in retina of newborn mice

doi:10.16098/j.issn.0529-1356.2018.06.002

Abstract

Abstract:

Objective To explore the effects of heroin on oxidative damage and specific protein expression in mice retina. Methods Sixty mice were randomly divided into 2 groups：the control group and the heroin groupThe mice of heroin group were injected intraperitoneally with heroin of the increasing doses (0.0571 g/kg, 0.0667 g/kg, 0.0706 g/kg) continuous(1-5, 6-10 and 11-15 days) for 15 days(twice a day)，and the control group was injected with equivalence salineactivities of lactic dehydrogenase（LDH）and content of glutathione（GSH）in plasma，activities of inducible nitric oxide synthase (iNOS) in retinal tissue were detected by colorimetryThe expressions of Caspase-3 and nuclear factor-κB（NF-κB) protein of retina were examined by immunohistochemistry method. Results Compared with control group，the content of plasma GSH remarkable lower in heroin group. Meanwhile，the activities of plasma LDH remarkable rise in heroin group，the activities of mice retina iNOS remarkable increase in heroin group. The positive expressions of Caspase-3，NF-κB protein in retina were significantly increased. Conclusion Heroin may effect on GSH content and LDS activities in plasma and activity of iNOS in retinal tissue，induces the expressions of Caspase-3 and NF-κB protein of retinal cells，to influence the structure of the animal retina.

Key words: Heroin, Retina, Inducible nitric oxide synthase, Caspase-3, Nuclear factor-κB, Immunohistochemistry, Mouse

LI Chong-yang GOU Tao-ran LIU Ting-ting YU Shi-yuan . Effects of heroin on oxidative damage and specific protein expression in retina of newborn mice[J]. Acta Anatomica Sinica, 2018, 49(6): 708-713.

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