Localization and expression analysis of brain-derived neurotrophic factor and nitric oxide synthase in the eyeball of Chinese alligator

doi:视网膜| 脑源性神经营养因子| 一氧化氮合酶| 免疫组织化学| 扬子鳄

Abstract

Abstract:

Objective To explorethe expression and distribution of nitric oxide synthase (NOS) and brain-derived neurotrophic factor (BDNF) in the egeball of Chinese alligator,as well as the relationship between them in the eyeball. Methods The homology of amino acid sequence of BDNF or NOS proteinfromhuman, Oryctolaguscuniculus, Xenopuslaevis and Danioreriowas analyzed by bioinformatics.The distribution sites of NOS and BDNF in the alligator’s eyeball were detected using HE staining,immunohistochemistry and immunofluorescence, respectively. Results Both the choroid layer and the layer of the retina found the BDNF expressing in the alligator’s eyeball.The NOS immunopositive was abundantly expressed in the ganglion cell layer of the retina. The BDNF and NOS were both founded in the ganglion cell layer and the inner boundary layer of the retina. Conclusion BDNF and NOS are expressed in the eyeballs of Chinese alligators, which may be related to the adjustment of visual formation.

Key words: Retina, Brain-derived neurotrophic factor, Nitric oxide synthase, Immunohistochemistry, Chinese alligator

HUANG Yan-jiao ZHENG Lan-rong ZHOU Yong-kang. Localization and expression analysis of brain-derived neurotrophic factor and nitric oxide synthase in the eyeball of Chinese alligator[J]. Acta Anatomica Sinica, 2019, 50(5): 561-564.

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URL: https://jpxb.bjmu.edu.cn/EN/视网膜| 脑源性神经营养因子| 一氧化氮合酶| 免疫组织化学| 扬子鳄

https://jpxb.bjmu.edu.cn/EN/Y2019/V50/I5/561

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