Objective  To investigate the effects of microRNA-4286 (miRNA-4286) on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ (INPP4A).    Methods  HGC-27 cells were divided into blank control group, negative control group and intervention group. The blank control group received no treatment, negative control group was transfected with pEGFP-N1 plasmid, and intervention group was transfected with pEGFP-N1-miRNA-4286 plasmid. Real-time PCR was used to detect the expression of miRNA-4286 in HGC-27 cells, and Western blotting was used to detect the expression of INPP4A protein in HGC-27 cells. The proliferation rate, invasion ability and migration ability of HGC-27 cells were detected by cholecystokinin octapeptide, cholecystokinin octapeptide(CCK8), Transwell chamber assay and scratch test, respectively.    Results  The miRNA-4286 expression level, proliferation rate, and number of transmembrane cells in intervention group were significantly higher than those in blank control group and negative control group (P<0.05). The expression level of INPP4A protein and the scratch healing ability of intervention group were significantly lower than those of blank control group and negative control group (P<0.05). The miRNA-4286 expression, INPP4A protein expression, proliferation rate, number of transmembrane cells, and the scratch healing ability of negative control group had no significant difference with those of blank control group (P>0.05).    Conclusion  miRNA-4286 may promote the growth, migration and invasion of gastric cancer cell line HGC-27 by down-regulating the expression of INPP4A.