Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901#br#

doi:10.16098/j.issn.0529-1356.2021.05.012

Abstract

Abstract:

Objective To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin (0.1, 1, 2 and 4 mmol/L) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase(MMP)-2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9, intercellular cell adhesion molecule-1(ICAM-1) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase(p-MKK)3/6 were detected by Western blotting. Results Melatonin inhibited the migration and invasion of gastric cancer cells in a dose-dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells. Conclusion Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway. 

Key words: Melatonin, Gastric cancer, Matrix metalloproteinase-2, Matrix metalloproteinase-9, Intercellular cell adhesion molecule-1, CD44, P38, Western blotting

CLC Number:

R735.2

XU Li JIN Qing-dong CHEN Xiu-jiao CHEN Chao ZHENG Wei-nan ZHOU Rui-xiang. Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901#br#[J]. Acta Anatomica Sinica, 2021, 52(5): 751-758.

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