Solute carrier family 6 member 1 promoting the proliferation and invasion of breast cancer cells through affecting the phosphorylation of PI3K/Akt signaling pathway

doi:10.16098/j.issn.0529-1356.2021.05.013

Abstract

Abstract:

Objective To investigate the effects of solute carrier family 6 member 1 (SLC6A1) on migration and invasion of breast cancer cells and its molecular mechanism. Methods Data sets GSE125989 and GSE100534 were downloaded and comprehensively analyzed to screen the key genes. Protein interaction network of key genes was constructed by STRING database and Cytoscape 3.6.1 software, and hub genes were screened. Expression of hub gene SLC6A1 in breast cancer and its effect on prognosis were detected by Ualcan datebase and GEPIA datebase. SLC6A1-siRNA plasmid was transfected to interfere with SLC6A1 expression in breast cancer cells. Migration and invasion of breast cancer cells were detected by wound healing assay and Transwell assays. The mechanism of SLC6A1 in breast cancer was detected by gene set enrichment analysis and Western blotting. SC79 was used to activate the Akt pathway, and then detect changes in cell migration and invasion were detected. Results A total of 92 differentially expressed genes between metastatic breast cancer and carcinoma in situ were selected, and 20 hub genes including collagen Ⅰα1(COL1A1),vascular endothelial growth factor A(VEGFA) and SLC6A1 were selected. SLC6A1 were highly expressed in breast cancer tissues (P<0.001) and correlated with prognosis (P=0.01). After SLC6A1 interference, the migration and invasion of breast cancer cells decreased significantly (P<0.05). Inhibition of SLC6A1 expression can inhibit the phosphorylation of PI3K/Akt signaling pathway (P<0.05). Activation of the PI3K/Akt pathway eliminated the inhibitory effect of SLC6A1-siRNA on migration and invasion of breast cancer cells (P<0.05). Conclusion SLC6A1 promotes the migration and invasion of breast cancer cells through the PI3K/Akt signaling pathway.

Key words: Breast cancer, Solute carrier family 6 member 1, Migration, Invasion, Phosphatidylinositol 3-kinase/protein kinase B, Western blotting, Human

CLC Number:

R737.9

JI Lei XU Yang TANG Nan. Solute carrier family 6 member 1 promoting the proliferation and invasion of breast cancer cells through affecting the phosphorylation of PI3K/Akt signaling pathway[J]. Acta Anatomica Sinica, 2021, 52(5): 759-766.

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