Expression and role of CCAAT enhancer binding protein β mRNA, microRNA-369-3p and rno-Rmdn2_0006 of hepatocytes during the rat liver regeneration initiation

doi:10.16098/j.issn.0529-1356.2021.06.011

Abstract

Abstract:

Objective To explore the pathways and patterns which CCAAT/enhancer binding protein β(CEBPβ) mRNA, miR-369-3p and rno-Rmdn2_0006 regulate the hepatocytes in G0 phase and G1 phase during rat liver regeneration (LR). Methods The rat 2/3 partial hepatectomy (PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method of Smedsrod et al, the large-scale quantitative detection of competitive endogenous RNA(ceRNAs) was processed by the high-throughput biotechnology, the interaction network of ceRNAs was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNAs was analyzed by ceRNA comprehensive analysis. Results It was found that at 0 hour and 6 hour after PH, the ratio value of CEBPβ mRNA showed 1.11±0.11 and 2.57±0.10, miR-136-3p displayed 0.70±0.22 and 0.28±0.03, rno-Rmdn2_0006 indicated 1.26±0.34 and 2.62±0.70. The G0 phase-related genes promoted by CEBPβ were following, the growth arrest and DNA damage inducible beta (GADD45β) 0.12±0.09 and 2.50±0.44, the cyclin dependent kinase inhibitor 1A(CDKN1A）0.39±0.07 and 0.93±0.15, but the G0 phase-related genes inbibited by CEBPβ were following, the cyclin dependent kinase 2 associated protein 2 (CDK2AP2) 2.55±0.42 and 0.74±0.11, the signal transducer and activator of transcription 1 (STAT1) 2.57±0.13 and 1.32±0.13. The G1 phase-related genes promoted by CEBPβ were following, the ETS variant transcription factor 6 (ETV6) 0.77±0.05 and 2.22±0.68, the hemoglobin oxygenase 1 (HMOX1) 1.05±0.21 and 4.57±0.88, the mitogen-activated protein kinase 14 (MAPK14) 1.01±0.15 and 2.01±0.32, the thioredoxin interacting protein (TXNIP) 1.03±0.07 and 2.50±0.19, but the G1 phase-related gene nuclear factor erythroid 2 like 2 (NFE2L2) inbibited by CEBPβ 0.66±0.09 and 0.35±0.05. Conclusion CEBPβ mRNA is not up-regulated at 0 hour after PH, that is helpful for the expression of the G0 phase-related genes inbibited by CEBPβ and for the hepatocytes to be in G0 phase. On the contrary, the interaction of miR-369-3p and rno-Rmdn2_0006 leads to CEBPβ mRNA to bind with miR-369-3p, to CEBPβ being formed probablely, and to the expression of the G1 phase-related genes promoted by CEBPβ probablely, and to the hepatocytes being in G1 phase at 6 hours after PH.

Key words: Liver regeneration, Hepatocyte in G0 phase, , Hepatocyte in G1 phase, CCAAT/enhancer binding protein β, Competitive endogenous RNA comprehensive analysis, Biological hig-throughput detection, Rat

CLC Number:

Q257

BAI Ge WANG Zi-hui SONG Ya-ping ZANG Xia-yan LI Ya-fei YE Bing-yu ZHAO Zhi-hu XU Cun-shuan . Expression and role of CCAAT enhancer binding protein β mRNA, microRNA-369-3p and rno-Rmdn2_0006 of hepatocytes during the rat liver regeneration initiation[J]. Acta Anatomica Sinica, 2021, 52(6): 913-916.

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