Objective  To explore the pathways and patterns which the CCAAT enhancer binding protein δ(CEBPδ) mRNA, miR-3553 and rno-Acad8_0002 regulate the hepatocytes in G0 phase and G1 phase during rat liver regeneration (LR).    Methods  The rat 2/3 partial hepatectomy (PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method  of Smedsrod et al, a large-scale quantitative detection of competitive endogenous RNA(ceRNAs) was processed by the high-throughput biotechnology, the interaction network of ceRNAs was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNAs was analyzed by ceRNA comprehensive analysis.    Results  It was found that at 0 hour and 6 hours after PH, the ratio value of CEBPδ mRNA showed 0.40±0.08 and 2.15±0.24, miR-3553 displayed 2.53±0.47 and 1.17±0.31, rno-Acad8_0002 indicated 1.24±0.04 and 2.66±0.54. The G0 phase-related genes inbibited by CEBPδ were following, the transforming growth factor beta receptor 2 (TGFBR2) were 2.77±0.20 and 0.79±0.13, the phospholipase A2 group IVA (PLA2G4A) were 2.56±0.76 and 0.42±0.13. The G1 phase-related genes promoted by CEBPδ were following, the plasminogen activator urokinase receptor (PLAUR) were 0.27±0.08 and 2.62±0.31, the mitogen-activated protein kinase 14 (MAPK14) 1.01±0.15 and 2.01±0.32, the ETS variant transcription factor 6 (ETV6) were 0.77±0.05 and 2.22±0.68, the hemoglobin oxygenase 1 (HMOX1) were 1.05±0.21 and 4.57±0.88.   Conclusion  CEBPδ mRNA is down-regulated at 0 hour after PH, that is helpful for the expression of the G0 phase-related genes inhibited by CEBPδ and for the hepatocytes to be in G0 phase. On the contrary, the interaction of miR-3553 and rno-Acad8_0002 leads to CEBPδ mRNA to bind with miR-3553, to CEBPδ being formed probablely, and to the expression of the G1 phase-related genes promoted by CEBPβ probablely, and to the hepatocytes being in G1 phase at 6 hours after PH.