Expression and prognostic significance of targeting protein for xenopus kinesin-like protein 2 in hepatocellular carcinoma

doi:10.16098/j.issn.0529-1356.2023.04.009

Abstract

Abstract:

Objective To analyze the expression of targeting protein for xenopus kinesin-like protein 2(TPX2) in hepatocellular carcinoma (HCC) and its clinical prognostic significance. Methods First, the expression levels, survival prognosis and correlation of TPX2 in HCC were analyzed using UALCAN, K-PLOT and HPA databases. Secondly, the TIMER, GEPIA, and SangerBox databases were used to analyze the immune cell infiltration of TPX2, its correlation with TP53 mutation, and the mutation landscape map. Finally, the co-expressed genes of TPX2 in HCC and their prognostic value were analyzed by HCCDB database, and the co-expressed genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) were analyzed by the HCCDB database. Results TPX2 was highly expressed in HCC and was not conducive to overall survival(OS), disease-specific survival(DSS), progression-free survival(PFS), and recurrence free survival(RFS) of HCC patients; and its presence in HCC was significantly correlated with tumor cell purity and multiple immune cells (B cells, CD4⁺T, and CD8⁺T, etc.); Furthermore, the expression level of TPX2 in HCC was significantly correlated with TP53 mutation. The area under curve(AUC) under receiver operating characteristic(ROC) curve of TPX2 for the 1-year, 3-year and 5-year OS prognostic diagnosis in HCC patients was 0.73, 95% CI (0.669-0.79), 0.668, 95% CI (0.604-0.732) and 0.654,5% CI (0.574-0.734), respectively. Co-expressed genes of TPX2 were not conducive to OS of patient; its GO function was mainly enriched in mitosis and nuclear division, microtubule cytoskeleton, and KEGG was mainly enriched in cell cycle, oocyte meiosis and p53 signaling pathways, and AUC of the 1-year, 3-year and 5-year OS prognostic for HCC patients was 0.801, 95% CI (0.745-0.856), 0.725, 95% CI (0.667-0.786) and 0.711, 95% CI (0.635~0.788). Conclusion TPX2 is closely related to the occurrence of HCC, which is not conducive to the survival and prognosis of HCC patients, and can be served as a biomarker for the diagnosis and prognosis of HCC.

Key words:

Targeting protein for xenopus kinesin-like protein 2, Disease-free survival, Hepatocellular carcinoma, Bioinformatics, Database analysis

CLC Number:

R365

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