Table of Content

2023, Volume 54 Issue 4
06 August 2023 Previous Issue    Next Issue
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Neurobiology
Anthropology
Review
Cancer Biology
Anatomy
Cell and Molecules Biology


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Neurobiology

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Effects of astrocytes on the proliferation of neural stem cells in the hippocampal microenvironment of old and young adults CHENG Yong-bo QU Jia-hua SHAN Wen LIU Qian-qian QIN Jian-bin TIAN Mei-ling ZHANG Xin-hua SHI Wei 2023, 54 (4):  375-382.  doi: 10.16098/j.issn.0529-1356.2023.04.001 Abstract ( )   PDF (11268KB) ( )   Objective To investigate the effect and mechanism of astrocytes on the proliferation of neural stem cells (NSCs) in adult and juvenile hippocampus microenvironment.    Methods  Hippocampal astrocytes were isolated and cultured from 5 female SD rats at day 1 and week 30 postnatal, respectively; Embryonic hippocampus NSCs was isolated and cultured from 1 SD rat at day 15 of gestation; Conditioned astrocyte culture medium（CM） was collected for NSCs culture; Flow cytometry and CCK-8 were used to detect the proliferation of NSCs cultured in CM. Colony stimulating factor 1 (CSF-1) with differential expression was screened by mass spectrometry after cultured astrocyte CM. Western blotting and ELISA were used to verify the result  of mass spectrometry. Immunofluorescence, flow cytometry and CCK-8 were used to detect the proliferation of NSCs treated with different concentrations of CSF-1 recombinant protein （20 μg/L,  100 μg/L, 1 mg/L and 5 mg/L）.    Results  Compared with the adult group, the CM of hippocampal astrocytes in the young group could promote the proliferation of NSCs(P<0.01); Compared with the conditioned medium of hippocampal astrocytes in the juvenile group, the expression of CSF-1 in the hippocampus of the elder group was significantly up-regulated(P<0.01); At 20 μg/L, CSF-1 promoted the proliferation of NSCs(P<0.01), and 5 mg/L CS-1 inhibited significantly the proliferation of NSCs(P<0.01).     Conclusion The secretion of CSF-1 by astrocytes in hippocampal microenvironment can regulate the proliferation of NSCs with the development of the times. References | Related Articles | Metrics

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Developmental comparison between cerebral organoids in vitro and body’s cortices in vivo FAN Wen-juan CHEN Xu-dong CHEN Yong-fang YANG Xu-guang JIN Shao-ju ZHAO Zhi-jun DENG Jin-bo 2023, 54 (4):  383-391.  doi: 10.16098/j.issn.0529-1356.2023.04.002 Abstract ( )   PDF (19209KB) ( )   Objective To understand the characteristics and developmental differences between cerebral organoids in vitro and normal cerebral cortices in vivo.   Methods  1. Grouping: cerebral cortices in vivo group and cultured cerebral organoids in vitro group. 2.  Sample collection: cortical tissues were collected from Kunming mouse embryos at embryonic day 7.5（E7.5）, E9.5, E11.5, E14.5, and postnatal day 3 (P3) or P7. Three specimens were taken from each group. Meanwhile, cerebral organoids were cultured with mouse induced pluripotent stem cells (iPSCs), and samples at different culture time point were collected, and more than 3 samples were collected at each time point. 3. Detection method: the distribution of different types of cells in each group of specimens was analyzed by immunofluorescent staining.  Results  While relative similarities between in vivo cerebral cortical development and the cerebral organoids in vitro were observed, including the histogenesis, and the morphological differentiation of nerve cells and glial cells, the lamellar architecture of cerebral cortex in mouse brain was not observed in cerebral organoids.     Conclusion  The development of cerebral organoids in vitro has some similarity with body’s cortical development. Therefore, cerebral organoids can be used to a substitution of cortex and diseases’ models, but improvement of the existing technologies is necessary.  References | Related Articles | Metrics

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Repairing effect of sulodexide on diabetic retinopathy and the regulation of MAPK pathway in rats ZHUANG Hai-rong WU Zi-dong CHEN Xue-hong LI Cheng-jun 2023, 54 (4):  392-399.  doi: 10.16098/j.issn.0529-1356.2023.04.003 Abstract ( )   PDF (8897KB) ( )   Objective  To study the effect of sulodexide on the repair of diabetic retinopathy and the regulation of MAPK pathway in rats.    Methods  Totally 72 rats were randomly divided into normal control group, diabetic retinopathy group, low, middle and high dose of sulodexide group and metformin hydrochloride group. Except normal control group, other rats were intraperitoneally injected with streptozotocin to establish the rat model of diabetic retinopathy. Rats in the low, middle and high dose sulodexide groups were given sulodexide by intragastric administration of 10 mg/kg，20 mg/kg and 40 mg/kg, respectively. Metformin hydrochloride group was given metformin hydrochloride of 200 mg/kg, once a day for 12 weeks. The levels of fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c) and serum levels of advanced glycation end products (AGEs), interleukin-6 (IL-6), IL-1β, and levels of glucose transporter 1 (GLUT-1), glucose transporter 3(GLUT-3), superoxide dismutase (SOD) and malondialdehyde (MDA) in retina were detected. The levels of p38 MAPK and phosphorylated p38 MAPK (p-p38 MAPK) in retina were detected by immunohistochemistry and Western blotting. Retinal pathological changes and ganglion cell count were examined by HE staining.    Results The levels of FBG and HbA1c, serum AGEs, IL-6, IL-1 β, GLUT-1, GLUT-3, MDA and p38 MAPK mRNA, p38 MAPK, p-p38 MAPK/p38 MAPK and immunohistochemical integral optical density of retina in sulodexide group were significantly lower than those in diabetic retinopathy group (P<0.05), while the SOD level and ganglion cell number in retinal tissue were significantly higher than those in diabetic retinopathy group (P<0.05).    Conclusion  Sulodexide can regulate blood glucose level and retinal glucose metabolism in diabetic retinopathy rats, and repair retinal pathological damage, and its mechanism may be related to the regulation of MAPK pathway. References | Related Articles | Metrics

Anthropology

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Digit ratio in men with schizophrenia NIU Shi-bo YANG Meng-yi XU Yu DANG Jie MA Zhan-bing HUO Zheng-hao LU Hong 2023, 54 (4):  473-476.  doi: 10.16098/j.issn.0529-1356.2023.04.014 Abstract ( )   PDF (722KB) ( )   Objective To investigate the relationship between digit ratio and schizophrenia in men from Ningxia Han nationality.    Methods Using anthropometry, digital camera was used to obtain finger photographs of both hands of 216 male subjects (normal control: 116, schizophrenia patient: 100). The anatomical points were marked and the length of each finger of both hands were measured by computer image analysis software (Image-Pro Plus 6.0). Differences of the mean digit ratio of left and right hand 2D∶3D, 2D∶4D, 2D∶5D, 3D∶4D, 3D∶5D, 4D∶5D between the control group and the patient group were compared. The correlation between 2D∶4D and onset age in the patient group were analyzed.   Results Except for left and right hand 3D∶4D, digit ratios in patient group were significantly higher than those in control group (2D∶4D and 3D∶5D in both hands, right hand 3D∶5D, p all<0.001; 2D∶3D and 4D∶5D, left hand 3D∶5D, P <0.01). Compared with the control group (left hand: 1%, right hand: 1%), the percentage of 2D∶4D ≥1 were significantly higher (P <0.05) in the patient group (left hand: 6%, right hand: 7%). There were no correlations between 2D∶4D mean value and onset age in patients group (P >0.05).  Conclusion Digit ratio is related to the occurrence of schizophrenia in Ningxia Han male. References | Related Articles | Metrics

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Six body indexes and typing characteristics of Xibe adults XU Guo-chang CHEN Zhi-guo WEN You-feng JIANG Dong GUO Ke-lei 2023, 54 (4):  477-483.  doi: 10.16098/j.issn.0529-1356.2023.04.015 Abstract ( )   PDF (780KB) ( )   Objective To explore the body index and typing characteristics of Xibe nationality.    Methods According to Anthropomatric Methods, height, chest circumference, sitting height, shoulder width, pelvic width and body mass morphological indexes were collected from 588 Xibe people in Yili area and 420 Xibe people over 18 years old in Shenyang area. After data collation, body index and typing were calculated and analyzed statistically.    Results Except for Xibe male stature-shoulder breadth index and Caup’ s index in Yili area and female stature-shoulder breadth index in Shenyang area, there were differences among age groups in other indexes (P <0.05). Except for male stature-chest circumference index, staturecrista iliaca index, female stature-chest circumference index, stature-sitting height index, Manouvrier’s skelic index, there were differences among regions (P <0.05). The adult body shape of Xibe nationality was mainly of wide chest, wide shoulder, long trunk, wide pelvis, medium leg and sub-long leg (male), sub-short leg and medium leg (female), and overweight.    Conclusion There are regional and age differences in the body index and typing of Xibe adults. Compared with other ethnic minorities, Xibe nationality’s stature-chest circumference index, stature-shoulder breadth index, stature-crista iliaca index, Caup’s index are at a higher level. The proportion of legs and body of Xibe nationality is in the middle level, while the proportion of trunk is slightly shorter for men and slightly longer for women.  References | Related Articles | Metrics

Review

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Research progress in the reprogramming of retinal Müller glia cells ZHANG Xiao-li ZHANG Shu-qiang XU Hui 2023, 54 (4):  484-489.  doi: 10.16098/j.issn.0529-1356.2023.04.016 Abstract ( )   PDF (1493KB) ( )   Blinding eye diseases caused by retinal degeneration have a detrimental effect on human health. Mammalian retina exhibits very limited capacity for self-repair after degenerative disease or injury. In contrast, zebrafish retina possesses a robust regenerative response that regenerates all types of retinal neurons and restores vision. Retina regeneration in zebrafish depends on a type of glia cells called Müller glia. Following retinal injury, zebrafish Müller glia undergo a reprogramming process and proliferate into multipotent progenitor cells that further differentiate into newborn retinal neurons. In recent years, significant progress has been made in the field of Müller glia-based retina regeneration. Here we summarize the mechanisms governing zebrafish retina regeneration and the recent advances in mammalian Müller glia reprogramming. References | Related Articles | Metrics

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Role and research progress of circular RNA in Alzheimer’s disease #br# #br# WANG Hongfang GENG Dan-dan ZHANG Run-jiao LIU Qing LI Yi-bo WANG Lei 2023, 54 (4):  490-494.  doi: 10.16098/j.issn.0529-1356.2023.04.017 Abstract ( )   PDF (3214KB) ( )   The circular RNA (circRNA) is a class of endogenous expressed non-coding RNA that are formed by covalently closed cyclization through reverse splicing. In recent years, a variety of highly conserved and cell-type specific circRNA have been identified in eukaryotes. Alzheimer’s disease (AD) is a common neurodegenerative disease and the most common cause of dementia in the elderly. Recent studies had shown that circRNA was involved in the pathogenesis and development of AD, such as amyloid β-protein (Aβ) metabolic, neuroinflammation, oxidative stress, autophagy and synaptic plasticity. The role and application value of circRNA in AD pathology are reviewed to provide a theoretical basis for the application of circRNA in the treatment and diagnosis of AD. References | Related Articles | Metrics

Cancer Biology

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Role and clinical significance of MLLT1 super elongation complex subunit in the occurrence and development of hepatocellular carcinoma YU Hua-jing WEI Lu-yang LIU Shan-shan GUAN Cheng-jian ZHANG Zhong-tao 2023, 54 (4):  425-433.  doi: 10.16098/j.issn.0529-1356.2023.04.008 Abstract ( )   PDF (19475KB) ( )   Objective  To investigate the role of MLLT1 in hepatocellular carcinoma （HCC）and its impact on the tumor immune microenvironment.    Methods  Multivariate Cox regression analysis and tumor gene analysis tools such as GEPIA and UALCAN were used to explore the expression of the MLLT1 gene and its prognostic significance in different tumors. Real-time PCR, Western blotting, and immunohistochemistry were used to investigate the differential expression of MLLT1 between HCC tumor tissue and normal tissue. MTT assay and cell cycle analysis were performed to assess the effect of MLLT1 knockdown on cell proliferation and cell cycle. The correlation between MLLT1 and immune cells, as well as immune infiltrates in the tumor microenvironment, and their correlation with immune neoantigens, immune checkpoints, tumor mutation burden, and microsatellite instability were also explored.    Results  The MLLT1 gene was found to be aberrantly expressed in various solid tumors including HCC, and its high expression was associated with poor prognosis in HCC. Knockdown of MLLT1 inhibited HCC cell proliferation and blocked the cell cycle. High expression of MLLT1 was found to affect the content of multiple immune cells, including CD4+T cells and neutrophile granulocyte cells in the HCC microenvironment.    Conclusion  MLLT1 is highly expressed in HCC and knockdown of MLLT1 can inhibit HCC cell proliferation and block the cell cycle. MLLT1 has a certain degree of impact on the immune microenvironment of HCC. Therefore, MLLT1 may serve as a potential diagnostic biomarker and therapeutic target for HCC. References | Related Articles | Metrics

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Expression and prognostic significance of targeting protein for xenopus kinesin-like protein 2 in hepatocellular carcinoma 2023, 54 (4):  434-444.  doi: 10.16098/j.issn.0529-1356.2023.04.009 Abstract ( )   PDF (18007KB) ( )    Objective  To analyze the expression of targeting protein for xenopus kinesin-like protein 2(TPX2) in hepatocellular carcinoma (HCC) and its clinical prognostic significance.    Methods  First, the expression levels, survival prognosis and correlation of TPX2 in HCC were analyzed using UALCAN, K-PLOT and HPA databases. Secondly, the TIMER, GEPIA, and SangerBox databases were used to analyze the immune cell infiltration of TPX2, its correlation with TP53 mutation, and the mutation landscape map. Finally, the co-expressed genes of TPX2 in HCC and their prognostic value were analyzed by HCCDB database, and the co-expressed genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) were analyzed by the HCCDB database.    Results  TPX2 was highly expressed in HCC and was not conducive to overall survival(OS), disease-specific survival(DSS), progression-free survival(PFS), and recurrence free survival(RFS) of HCC patients; and its presence in HCC was significantly correlated with tumor cell purity and multiple immune cells (B cells, CD4+T, and CD8+T, etc.); Furthermore, the expression level of TPX2 in HCC was significantly correlated with TP53 mutation. The area under curve(AUC) under receiver operating characteristic(ROC) curve of TPX2 for the 1-year, 3-year and 5-year OS prognostic diagnosis in HCC patients was 0.73, 95% CI (0.669-0.79), 0.668, 95% CI (0.604-0.732) and 0.654,5% CI (0.574-0.734), respectively. Co-expressed genes of TPX2 were not conducive to OS of patient; its GO function was mainly enriched in mitosis and nuclear division, microtubule cytoskeleton, and KEGG was mainly enriched in cell cycle, oocyte meiosis and p53 signaling pathways, and AUC of the 1-year, 3-year and 5-year OS prognostic for HCC patients was 0.801, 95% CI (0.745-0.856), 0.725, 95% CI (0.667-0.786) and 0.711, 95% CI (0.635~0.788).      Conclusion  TPX2 is closely related to the occurrence of HCC, which is not conducive to the survival and prognosis of HCC patients, and can be served as a biomarker for the diagnosis and prognosis of HCC. References | Related Articles | Metrics

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Screening ferroptosis related genes influencing prognosis of colon cancer through bioinformatics analysis LI Xiao-jun ZHANG Ya-min 2023, 54 (4):  445-452.  doi: 10.16098/j.issn.0529-1356.2023.04.010 Abstract ( )   PDF (15013KB) ( )   Objective  To explore ferroptosis-related long non-coding RNAs (lncRNAs) with prognostic significance in colon cancer (CC), and then construct a prognosis-related predictive scoring model. To search for ferroptosis-related differential expressed genes co-expressed with prognosis-related lncRNAs.    Methods  Ferroptosis-related genes (FGs) were downloaded from FerrDb database; The expression data of 41 adjacent normal tissues and 473 tumor tissues, and clinical data of 452 patients were successfully downloaded. Co-expression and differential expression analysis was performed to identify differentially expressed ferroptosis-related lncRNAs (DEFlncRNAs), and univariate Cox regression analysis was used to screen statistically significant prognosis-related DEFlncRNAs, and then multivariate Cox regression analysis was used to construct a prognostic model, calculate risk score among CC patients and divide patients by the median risk score. Kaplan-Meier curves, univariate and multivariate Cox regression analyses, and receiver operationg characteristic(ROC) curve were used to reveale great accuracy of the model. Then, a nomogram was drawed to predict the survival among CC patients. Finally, the differentially expressed ferroptosis-related genes regulating DEFlncRNAs were found by co-expression analysis, and the different expression was verified by immunohistochemical experiments.    Result  Expression and clinical data among colon cancer (CC) patients were downloaded from TCGA database. A risk prognostic model containing 28 lncRNAs to predict the prognosis among CC patients was successfully constructed. An effective clinical nomogram for predicting the overall survival of CC patients was successfully constructed. Finally, the co-expression analysis of DEFlncRNAs and differentially expressed ferroptosis-related genes (DEFGs) was preformed to obtain a co-expression network, including17 key DEFGs, with the correlation coefficient filter criteria (|corFilter|)>0.4 and  P value filter criteria (P value filter)<0.05. Immunohistochemical experiments confirmed ANGPTL7 was highly expressed in the adjacent tissues among CC patients.    Conclusion  Successfully constructed a prognostic-related model among CC patients containing 28 DEFlncRNAs, and 17 DEFGs was finally obtained.  References | Related Articles | Metrics

Anatomy

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Classification and clinical significance of the branching patterns of the right pulmonary arteries based on the intravital CT data LIU Hai-lan MEI Yi-xuan ZHANG Yi LIU Cheng LIU Shu-wei 2023, 54 (4):  453-459.  doi: 10.16098/j.issn.0529-1356.2023.04.011 Abstract ( )   PDF (8313KB) ( )   Objective  To track and make statistics of the right pulmonary arteries’ branches and to study the clinical significance.   Methods  Technologies of vascular volume rending were used to analyze the 350 CT pulmonary angiography images. And the three-dimensional display was used to classify the pulmonary arteries.    Results  According to the 350 reconstructed result, the right upper pulmonary arteries were divided into 6 types: anterior trunk+ post ascending artery, anterior trunk, anterior trunk+ anterior ascending artery+ post ascending artery, superior anterior trunk + inferior anterior trunk+ post ascending artery, anterior trunk+ anterior ascending artery and superior anterior trunk+ inferior anterior trunk. The right middle pulmonary arteries were divided into 2 types: one branch  and two branches. As for the right lower pulmonary arteries, the apical arteries were divided into 3 types: one branch, two branches and three branches. The basal segmental arteries were divided into 3 types: medial basal segmentalartery+anterior basal segmental artery+lateroposterior basal segmental artery, medioanterior basal segmental artery+lateroposterior basal segmental artery and medial basal segmental artery+anterior and lateral basal segmental artery+posterior basal segmental artery. To be specific, anterior trunk+ post ascending artery type (56.6%) occupied the most in the right upper pulmonary arteries. The majority type of right middle pulmonary artery was one branch(57.4%). In the right lower pulmonary arteries, apical artery of the lower lobe (one branch)+medial basal segmental artery+anterior basal segmental artery+lateroposterior basal segmental artery type（32.5%）was most common.    Conclusion  The classification of the right pulmonary arteries based on the three dimensional reconstruction of CT pulmonary angiography is of significant to surgical precision therapy of lung tumor. References | Related Articles | Metrics

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Linear measurement of digital pelvic of normal Tibetan nationality female in Lhasa  ZHONG Hua WANG Ying FAN Ying-lei REN Hai-long PEMA Dor-ji TONG Qing-hua 2023, 54 (4):  460-464.  doi: 10.16098/j.issn.0529-1356.2023.04.012 Abstract ( )   PDF (5562KB) ( )   Objective  To explore the range of normal female pelvic diameter lines in Tibetan nationality.    Methods  The subjects were Tibetan nationality female who underwent pelvic CT examination in the Radiology Department from January 2016 to December 2019 at the General Hospital of the Tibet Military Region of PLA. Ninty-six samples between the ages of 22 and 65 years, with an average age (46.06±11.42)years were recruited. Mimics 19.0 software was used to construct the digital three-dimensional model of pelvis, and to measure transverse diameter(TD), obstetric conjugate(OC), interspinous distance(ISD), sagittal midpelvic diameter(SMD), intertuberous distance(ITD), sagittal outlet diameter(SOD),diagonal conjugate diameter(DCD),sacrum length(SL), penal height(PH).Analysis of variance was used to compare different age groups, and Pearson correlation analysis was used to analyze the relationship between age and pelvic diameter.    Results  The linear measurement of TD was (132.08±6.15) mm, OC was (112.44±9.43) mm, ISD was (107.30±8.70), SMD was (129.06±7.73) mm, ITD was (123.02±12.08) mm, SOD was (118.80±8.87) mm, DCD was (127.49±9.80) mm, SL was (102.56±10.88) mm and PH was (36.57±4.57) mm.Cluster analysis showed that Lhasa Tibetans were closest to Uygurs.    Conclusion  The close clustering relationship between Tibetans and Uygurs in Lhasa suggests that there is a possibility of gene exchange between Tibetans and Uygurs in Hotan area in ancient times. The pelvic diameter of Tibetan women in Lhasa has changed significantly. Narrower OC, SL and PH make the pelvis flat, which is more and more detrimental to natural childbirth. References | Related Articles | Metrics

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Susceptibility weighted imaging of superficial cerebellar veins YAO Xiao-xiao LIU Xiao-li LI Ru-hua LI Chang-sheng REN Chuan-gen CHEN Cheng-chun 2023, 54 (4):  465-472.  doi: 10.16098/j.issn.0529-1356.2023.04.013 Abstract ( )   PDF (11908KB) ( )   Object Visualizing the superficial cerebellar vein and its tributaries on suscepxibility weighted imaging(SWI), and to construct superficial cerebellar vein network.    Methods  According to the inclusion criteria, 80 healthy volunteers (40 males and 40 females) were selected for 3.0T MRI scans to obtain conventional sequence cross-section, sagittal tomographic images, and SWI image data. Post-processing was performed on the Extended MR workspace 2.6.3.4 image workstation to reconstruct minimum intensity projection(mIP) images. SPSS 21.0 statistical software was used to analyze and process each data, and the diameter measurement result  were expressed as mean ± standard deviation.    Results Both SWI and mIP could image the structures of the cerebellum and its veins. The cerebellar veins were divided into deep and superficial parts. The superficial cerebellar veins were divided into two groups: the vermis and the cerebellar hemispheres. The superficial vein of the cerebellar vermis consisted of superior vermis vein［diameter: (1.21±0.24)mm, occurrence rate: 92.16%］, summit vein［diameter:(0.66±0.05)mm, occurrence rate: 95%］, mountain vein［diameter: (0.76±0.03)mm, occurrence rate: 100%］, inferior vermis vein［diameter: (1.40±0.27)mm, occurrence rate: 99.02%］. The superficial cerebellar hemisphere vein consists of anterior superior cerebellar vein ［diameter: (1.09±0.12)mm, occurrence rate: 100%］, posterior superior cerebellar vein ［diameter: (0.88±0.13)mm, occurrence rate: 70%］, anterior inferior cerebellar vein［diameter: (1.34±0.15)mm, occurrence rate: 100%］, posterior inferior cerebellar vein［diameter: (1.11±0.09)mm, occurrence rate: 92.5%］. The deep veins were divided into cerebellomesencephalic fissure group, cerebellopontine fissure group, and cerebellomedullary fissure group.   Conclusion SWI can display the microstructure and venules of the cerebellum, and can construct a network of superficial cerebellar veins. References | Related Articles | Metrics

Cell and Molecules Biology

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Distribution characteristics of polymorphisms of microRNA-30 gene in Guangxi Zhuang population and their association with serum lipid levels LUO Yan-ping LIU Chao GU Xi-xi CHEN Jian-ming LAN Yan WEI Ye-sheng 2023, 54 (4):  400-404.  doi: 10.16098/j.issn.0529-1356.2023.04.004 Abstract ( )   PDF (1273KB) ( )   Objective  To explore the distribution situation of microRNA(miR) -30 gene single-nucleotide sites rs1192037A/T polymorphisms in Guangxi Zhuang population and compare its distribution differences with other populations and to analyze level of common blood lipid indexes in genotypes.    Methods  SNPscan was used to detect rs1192037A/T locus genotyping in 236 volunteers of Zhuang nationality in Guangxi. The genotypes and allele frequencies of rs1192037A/T locus genotyping in different genders and groups were analyzed. The levels of common blood lipids in the subjects were detected by roche automatic biochemical apparatus.    Results  Three genotypes of AA, AT and TT were found in rs1192037 A/T with the frequency distribution of 11.0%, 38.6% and 50.4%, respectively.  No significant differences in genotypes and alleles frequencies of rs1192037 A/T  between different genders in Guangxi Zhuang population were observed (P>0.05). However,there were significant differences in the genotype and allele frequency of miR-30 gene rs1192037 A/T in Guangxi Zhuang population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published by HapMap  (P<0.05), and there were not significant difference in genotypes and allele frequencies in population of HCB and JTP (P>0.05). There were significant differences in the levels of TG among the 3 genotypes of rs1192037 A/T, and the TG levels of AT and TT genotypes were significantly higher than AA genotypes.    Conclusion  There are different degrees of rs1192037 A/T polymorphisms of miR-30 gene among Guangxi population and other ethnic populations and other regions. The polymorphism of rs1192037 A/T is related to the level of TG. References | Related Articles | Metrics

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Expression profiles and regulatory network of microRNA, long non-coding RNA and circular RNA in rat chronic stress depression model based on whole transcriptome technology MENG Pan ZHANG Xi YANG Hui LIU Jian FANG Rui WANG Xiao-ye GE Jin-wen LIU Tong-tong ZHAO Hong-qing WANG Yu-hong 2023, 54 (4):  405-413.  doi: 10.16098/j.issn.0529-1356.2023.04.005 Abstract ( )   PDF (8566KB) ( )   Objective  To explore the potential pathophysiological mechanism of depression by screening the expression profiles and competing endogenous RNA (ceRNA) regulatory network microRNA(miRNA), long non\|coding RNA(lncRNA) and circular RNA(circRNA) in the hippocampus of chronic stress depression rat model.    Methods  Twelve SD rats were divided into blank group and model group. Chronic mild unpredictability stress (CUMS) was used to construct the rat model of depression. The whole transcriptome analysis was performed on the hippocampus of the rats, and the possible regulatory networks among lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA were explored by bioinformatics method.    Results  According to the |fold change|≥1.5 and  P≤0.05, 29 differentially expressed miRNAs (21 up-regulated and 8 down-regulated), 686 differentially expressed lncRNAs (163 up-regulated and 523 down-regulated) and 8 differentially expressed circRNAs (3 up-regulated and 5 down-regulated) were identified. Gene Ontology(GO) and Kytot Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed that the target genes of miRNAs were mainly enriched in the Golgi apparatus and calcium ion binding process in the cell membrane, the functions of lncRNAs target genes involved nucleic acid binding regulation, cytokine and protein ubiquitination, etc, and the functions of host genes of circRNAs were associated with cellular stimulation response, metabolic process, catalytic activity and other processes. The ceRNA network of lncRNAs and circRNAs showed complex interactions between non-coding RNA (ncRNA) and mRNA related to synaptic plasticity, such as protein Wnt-sa(WNT5a) and collagentype Ⅲ alpha1(COL8a1) related to axon orientation and laminin A2(LAMA2) related to neurodevelopment.     Conclusion  The ceRNA network of lncRNA and circRNA shows that the complex interaction betweens ncRNA and mRNA is highly associated with the neuroplasticity, which support the neuroplasticity hypothesis of depression.  References | Related Articles | Metrics

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Expression change and role of myeloma cancer gene mRNA and the non-coding RNA in the hepatocyte cycle initiation and termination during the rat  liver regeneration XUE Qi-jie CHANG Cui-fang WANG Zi-hui ZANG Xia-yan LIN Kai-lin ZHANG Chun-bo HAN Lu YE Bing-yu XU Cun-shuan 2023, 54 (4):  414-419.  doi: 10.16098/j.issn.0529-1356.2023.04.006 Abstract ( )   PDF (2846KB) ( )   Objective  To explore the role pathway and pattern of the myeloma cancer gene (MYC) and its mRNA interaction with the microRNAs(miRNAs) and circular RNA(circRNAs) at hour 0, hour 6 and hour 72 in the rat liver regeneration.     Methods  The rat 2/3 hepatectomy (PH) model was prepared as described by Higgins, the hepatocytes were isolated according to the method  of Smedsrod et al. The expression changes of mRNA, miRNA and circRNA ［together named as competing endogenous RNA (ceRNA)］ were detected by the large-scale quantitative detection technology, the interaction network of ceRNA was constructed by Cytoscape 3.2 software, and their correlation in expression and role were analyzed by ceRNA comprehensive analysis.   Results  It was found that at hour 0 and hour 6 after PH, the ratio value of MYC mRNA showed 0.15±0.03 and 2.36±0.20, miR-134-5p indicated 3.22±0.61 and 0.08±0.02, circRNA_12112 displayed 0.68±0.21 and 13.35±3.53. At the same time, the cell cycle initiation-related genes ras association domain family member 1 (RASSF1), cyclin dependent kinase 2 (CDK2), superoxide dismutase 2 (SOD2), which were promoted in expression by MYC, were down-regulated at  hour 0 after PH, but the cell cycle initiation-related genes nestin (NES), RAD21 cohesin complex component (RAD21), CUE domain containing 2 (CUEDC2), which are inhibieted in expression by MYC, had no meaningful express changes at hour 0 after PH. On the other hand, the cell cycle initiation-related gene SOD2, which was promoted in expression by MYC, was upregulated at hour 6 after PH, but the cell cycle initiation-related genes NES, RAD21, CUEDC2, which are inhibieted in expression by MYC, were down-regulated at hour 6 after PH. In contrary, at hour 72 after PH, the ratio value of MYC mRNA showed 2.36±0.20, miR-880-3p indicated 0.54±0.01, circRNA_09599 displayd 0.54±0.16. At the same time, the cell cycle termination-related gene hepatocyte growth factor (HGF), which is promoted in expression by MYC, was up-regulated 72 hours after PH, the cell cycle termination-related genes MET proto-oncogene receptor tyrosine kinase (MET) and cyclin dependent kinase inhibitor 1A (CDKN1A), which are inhibieted in expression by MYC, were down-regulated 72 hours after PH.    Conclusion  The correlation in expression and role of the miRNAs, which are inhibited by circRNAs, MYC, its mRNA is inhibited by miRNAs, and the cell cycle initiation-related and cell cycle termination-related genes, which are regulated by MYC, are helpful for the hepatocyte to be in cell cycle initiation state at hour 6 after PH and to be in cell cycle termination state at hour 72 after PH. References | Related Articles | Metrics

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Expression change and role of Kruppel-like factor 4 mRNA, microRNA-881-3p, circular RNA_20298 and circular RNA_14826 in the hepatocyte apoptosis during the rat liver regeneration  LIN Kai-lin YANG Xian-guang WANG Zi-hui ZANG Xia-yan XUE Qi-jie HAN Lu ZHANG Chun-bo ZHAO Zhi-hu XU Cun-shuan 2023, 54 (4):  420-424.  doi: 10.16098/j.issn.0529-1356.2023.04.007 Abstract ( )   PDF (2042KB) ( )   Objective  To explore the role pathway and pattern of the Kruppel-like factor 4 (KLF4) and its mRNA interaction with microRNA(miRNAs) and circular RNA(circRNAs) at 0 hour and the 120 th hour in the rat liver regeneration.    Methods  The rat 2/3 hepatectomy (partial hepatectomy, PH) model was prepared as described by Higgins, the hepatocytes were isolated according to the method  of Smedsrod et al, the expression changes of mRNA, miRNA and circRNA together named as competing endogenous RNA (ceRNA) were detected by the large-scale quantitative detection technology, the interaction network of ceRNA  was constructed by Cytoscape 3.2 software, and their correlation in expression and role were analyzed by ceRNA comprehensive analysis.    Results  It was found that at the 0 hour and the 120th hour PH, the ratio value of KLF4 mRNA showed 1.00±0.16 and 3.14±0.27, miR-881-3p displays 18.30±1.44 and 0.47±0.02, circRNA_20298 indicated 0.32±0.10 and 4.24±0.22, circRNA_14826 showed 0.42±0.13 and 0.61±0.08. At the same time, the four kinds of cell apoptosis-related genes adrenoceptor beta 2 (ADRB2), dimethylarginine dimethylaminohydrolase 2 (DDAH2), annexin A5 (ANXA5), ect, which were promoted in expression by KLF4, were down-regulated at 0 hour after PH, but the cell apoptosis-related genes synuclein gamma (SNCG), glutathione-disulfide reductase (GSR), FYVE, RhoGEF and PH domain containing 4 (FGD4), ect, which were inhibited in expression by KLF4, were upregulated at 0 hour after PH. On the other hand, the cell apoptosis-related genes ANXA5 and thymosin beta 10 (TMSB10), which are promoted in expression by KLF4, were up-regulated at the 120th hour after PH, but the cell apoptosis-related genes chloride intracellular channel 4 (CLIC4) and ataxin 3 (ATXN3), ect, which were inhibited in expression by KLF4, were down-regulated at the 120th hour after PH.    Conclusion  The correlation in expression and role of the miRNAs, which are inhibited by circRNAs, KLF4, its mRNA is inhibited by miRNAs, and the cell apoptosis-related genes, which are regulated by KLF4, are helpful for the hepatocyte to be in active state 0 hour after PH and to be in apoptotic state 120-hour after PH. References | Related Articles | Metrics