Objective To explore the effect of scutellarin on lipopolysaccharide (LPS) induced neuroinflammation in BV-2 microglia cells.   Methods  BV-2 microglia were cultured and randomly divided into 6 groups: control group (Ctrl), cyclic GMP-AMP synthetase（cGAS）inhibitor RU320521 group (RU.521 group), LPS group, LPS+RU.521 group, LPS+ scutellarin pretreatment group (LPS+S) and LPS+S+RU.521 group. The expressions of cGAS, stimulator of interferon gene (STING), nuclear factor kappa B (NF-κB), phosphorylated NF-κB (p-NF-κB), neuroinflammatory factors PYD domainscontaining protein 3（NLRP3) and tumor necrosis factor α（TNF-α）in BV-2 microglia were detected by Western blotting and immunofluorescent double staining (n=3).   Results Western blotting and immunofluorescent double staining showed that compared with the control group, the expression of cGAS, STING, p-NF-κB, NLRP3 and TNF-α in BV-2 microglia increased significantly after LPS induction(P＜0.05), while the expression of cGAS, STING, p-NF-κB, NLRP3 and TNF-α in LPS+S group were significantly lower than those in LPS group (P＜0.05). Treatment with cGAS pathway inhibitor RU.521 showed similar effects as the pre-treatment group with scutellarin. In addition, the change of NF-κB in each group was not statistically significant（P＞0.05）.   Conclusion Scutellarin inhibits the neuroinflammation mediated by BV-2 microglia cells, which may be related to cGAS-STING signaling pathway.