Distribution and structural characteristics of the ventricular Purkinje fibers of yaks

doi:10.3969/j.issn.0529-1356.2013.01.015

Abstract

Abstract:

Objective To study the distribution and structural characteristics of the ventricular Purkinje fibers of 60 adult yaks, and to collect morphological data for the study of ventricular conduction system of plateau mammals. Methods The ink injection,ABS proplasm,HE staining,Masson ’s trichrome staining and immunohistochemical staining methods were used. Results The connective tissue sheath was found around the Purkinje fibres of the ventricle of yak. The left bundle branch of the yak heart had bi-fascicular or tri-fascicular structures in the subendocardial layer of the septum. The right bundle branch of the yak heart had tri-fascicular or quadric-fascicular structures in the myocardium at the root of the right anterior papillary muscle, by going through the moderator band.The Purkinje fibres that existed in the subendocardial layer presented a polygonal reticulate distribution, and extended out numerous branches to the myocardium. No Purkinje fiber was found on the top subendocardial layer of the papillary muscle. The configurations of the Purkinje fibers were scroll-shaped, honeycombed-shaped, funnel-shaped and ridge-shaped. Cx43 was found membrane positive in Purkinje fibres. Conclusion The ABS proplasm techonlogy can be used to study the distribution of Purkinje fibres. Left bundle branch and right bundle branch of yak heart are asymmetrically distributed, with the left bundle branch more developed.

Key words: Purkinje fibre, ABS proplasm, Ink injection, Immunohistochemistry, Yak

LI Chao-xu,DUAN De-yong,CUI Yan*. Distribution and structural characteristics of the ventricular Purkinje fibers of yaks[J]. AAS, 2013, 44(1 ): 79-84.

References

［1］ Durrer D, Van Dam RT, Freud GE, et al. Total excitation of the isolated human heart［J］. Circulation, 1970, 41(6):899-912.

［2］Myerburg RJ, Nilsson K, Gelband H. Physiology of canine intraventricular conduction and endocardial excitation ［J］. Circ Res, 1972, 30(2):217-243.

［3］Tawara S. The Conduction System of the Mammalian Heart: An Anatomicohistological Study of the Atrioventricular Bundle and the Purkinje Fibers［M］. London UK: Imperial College Press, 2000:8-160.

［4］Ansari A, Ho SY, Anderson RH. Distribution of the Purkinje fibres in the sheep heart［J］. Anat Rec, 1999, 254(1):92-97.

［5］King MR. The sino-ventricular system as demonstrated by the injection method［J］. Am J Anat, 1916, 19(2):149-177.

［6］Truex RC, Curry JL, Smythe MQ. Visualization of the Purkinje network of the beef heart［J］. Anat Rec, 1954, 118(4):723-735.

［7］Viragh S, Stoeckel ME, Porte A. Light and electron microscopic structure of the cardiac Purkinje fibers—review［J］. Physiol Bohemoslov, 1987,36(3):233-242.［8］ Cui Y, He YY, Wang J. Morphological characteristics of the ventricular microvasculature in adult yak［J］. Acta Theriologica Sinica, 2008,28(3):300-304.(in Chinese)

崔燕,贺延玉,王剑. 成年牦牛心室壁微血管的形态学特征［J］.兽类学报,2008,28(3):300-304. 

［9］ Hu JW, Cui Y, Yu SJ. Histological characteristics of coronary artery of yak in different ages［J］. Veterinary Science in China, 2010,40(6):631-635. (in Chinese)胡俊伟,崔燕,余四九. 不同年龄牦牛冠状动脉的组织学结构特征［J］.中国兽医科学,2010,40(6):631-635.

［10］Rentschler S, Vaidya DM, Tamaddon H, et al. Visualization and functional characterization of the developing murine cardiac conduction system［J］. Development, 2001,128(10):1785-1792.

［11］Kondo RP, Anderson RH, Kupershmidt S, et al, Development of the cardiac conduction system as delineated by mink-lacZ［J］. J Cardiovasc Electrophysiol, 2003,14(4):383-391.

［12］Miquerol L, Meysen S, Mangoni M, et al, Architectural and functional asymmetry of the His-Purkinje system of the murine heart［J］. Cardiovasc Res, 2004, 63(1): 77-86.

［13］Luetmer PH, Edwards WD, Seward JB, et al. Incidence and distribution of left ventricular false tendons: an autopsy study of 483 normal human hearts［J］. J Am Coll Cardiol, 1986,8(1):179-183. 

［14］Reig J, Alberti N, Petit M. Arterial vascularization of the human moderator band: an analysis of this structure’s role as a collateral circulation route［J］. Clin Anat, 2000,13(4):244-250.

［15］Truex RC, Copenhaver WM. Histology of the moderator band in man and other mammals with special reference to the conduction system［J］. Am J Anat, 1947,88(2):173-201.

［16］Hondeghem LM, Stroobandt R. Purkinje fibres of sheep papillary muscle. Occurrence of discontinuous fibres［J］. Am J Anat, 1974,141(2):251-262.

［17］Curran EJ. A constant bursa in relation with the bundle of His; with studies of the auricular connections of the bundle ［J］. Anat Rec, 1909,3(12):618-639.［18］DeWitt LM. Observations on the sino-ventricular connecting system of the mammalian heart［J］. Anat Rec, 1909,3(9):475-497.

［19］Field EJ. The development of the conducting system in the heart of sheep［J］. Br Heart, 1951,13(2):129-147.

［20］Beyer EC, Kistler J, Paul DL, et al. Antisera directed against connexin43 peptides react with a 43-kD protein localised to gap junctions in myocardium and other tissues［J］. Cell Biol, 1989,108(2):595-605.

［21］Pressler ML, Brodhecker CA, Huang XD, et al. Differential expression of gap junctional proteins in specialized and working myocardium［J］. Circulation, 1991, 84 (Suppl II):1290. 

[1]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[2]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[3]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[4]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[5]	YAN Kun WU Xiao-lin LIU Ying-juan GE Ke-li1 REN Lei-ming LI Hong-yun . Effect of cerebrotein hydrolysate-Ⅰon intestinal microflora regulation of mice with Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(5): 497-504.
[6]	ZHUANG Hai-rong WU Zi-dong CHEN Xue-hong LI Cheng-jun. Repairing effect of sulodexide on diabetic retinopathy and the regulation of MAPK pathway in rats [J]. Acta Anatomica Sinica, 2023, 54(4): 392-399.
[7]	YU Hua-jing WEI Lu-yang LIU Shan-shan GUAN Cheng-jian ZHANG Zhong-tao. Role and clinical significance of MLLT1 super elongation complex subunit in the occurrence and development of hepatocellular carcinoma [J]. Acta Anatomica Sinica, 2023, 54(4): 425-433.
[8]	WANG Xiao LI Liang CUI Cheng-li CAI Zhi-ping YU Chang ZHANG Li-jia . Endurance exercise combined with intermittent cold stimulation promoting white adipose tissue browning in rats [J]. Acta Anatomica Sinica, 2023, 54(3): 348-356.
[9]	ZHANG Yi-zhou LI Sha MI Shi-xiong ZUO Hong-chun ZHANG Qian-qian LI Han-lin LEI Zi-han ZHANG Dong-ze CUI Hui-xian. Effect of estradiol regulating sortilin-related receptor A expression on hippocampal spine density and synaptic protein expression via estrogen receptor of mouse [J]. Acta Anatomica Sinica, 2023, 54(3): 261-268.
[10]	LIU Ke-ting CHEN Bin-bin SHUI Yu-lian SHI Qing-ming XIAO Li. Neuroprotective effect of exogenous hydrogen sulfide and the expression of matrix metalloproteinases-9 and Caspase-1 in mice model of Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(3): 289-295.
[11]	LIU Xia ZHENG Li-ping DU Xiao-hua WANG Yu-jiao WU Ya-juan . Expression and localization of neuroglobin in the brain tissue of Saiga antelope [J]. Acta Anatomica Sinica, 2023, 54(2): 188-194.
[12]	LI Qiao LIU Xia DU Xiao-hua MI Xiao-yu WEN Yong-qiang FANG Ying-dong . Expression and localization of vascular endothelial growth factor-B in diencephalon and brainstem related tissues of the Yak’s brain [J]. Acta Anatomica Sinica, 2023, 54(1): 30-35.
[13]	SU Xiao-yun HE Ji-ping CUI Jian-mei SHI Xian-feng MEN Jie. Effects of estradiol on depressive-like behavior of ovariectomized rats and its possible mechanism [J]. Acta Anatomica Sinica, 2023, 54(1): 36-41.
[14]	GAO Sai-hong ZHANG Xiao-liang YANG Ying-chun QIAO Hai-bing. Activation of p38 MAPK and its effect on the expression of Bax and Bcl-2 after cerebral ischemia-reperfusion in hyperlipidemia rats [J]. Acta Anatomica Sinica, 2023, 54(1): 50-55.
[15]	TANG Xiao-lu DENG Cong-cong WANG Pei CHEN Ming-hui TIAN Kai SU Yan-yan MA Hui WU Xue-yan SHEN Yu-xian. Distribution of mesencephalic astrocyte derived neurotrophic factor in human, mouse and rat brain [J]. Acta Anatomica Sinica, 2022, 53(5): 551-556.