Neural protective effect of estrogen on the rat red nucleus by dissector quantitative analysis

doi:10.3969/j.issn.0529-1356.2013.04.004

Abstract

Abstract:

Objective To investigate the protective effect of estrogen(E₂) on red nucleus after spinal cord injury (SCI) inthe ovarectomized rat. Methods Eighty adult female rats were selected in this experiment and randomly divided into the normal group, rubrospinal tract injury group, estrogen replacement group, inhibitor of estrogen receptor (ICI) treatment group and E2+ICI cooperative treatment group. Sole rubrospinal tract injury model was established by cutting off the left dorsolateral funiculus at C3-C4 segment of rat spinal cord on one week after ovariectomy operation. The animal behavior was evaluated with forelimb probing experiment, and FR positive neurons were counted and analyzed on 1 week, 2 weeks and 4 weeks by using disecter after different treatments on animals. Results The forelimb probing test showed a higher use rate of left forelimb in the E₂replacement treatment group than that of other groups, but with no statistical significance (P>0.05). Except the normal group, the number of neurons in right red nucleus of rat midbrain decreased, especially on 4 weeks. The FR positive neurons of E₂ replacement group had a more remarkable cell body, clearer outline and longer processes compared to the neurons in the RST injury group, ICI treatment group and E2+ICI cooperative treatment group. The stereological frame illustrated that more FR positive neurons survived in the E₂ replacement group (P<0.05), but with no significant changes (P>0.05) among the RST injury group, ICI treatment group and E₂+ICI cooperative treatment group. Conclusion The E₂ replacement treatment may release red nucleus neuron retrograde injury and promote the neuron survival after rat SCI.

Key words: Estrogen, Red nucleus, Retrograde tracing, Disector, Rat

CLC Number:

R322.8-3

HAN Xiao JI Da-feng LI Yao-fu ZHANG Xia-nan QIN Jian-bing Lü Guang-ming*. Neural protective effect of estrogen on the rat red nucleus by dissector quantitative analysis[J]. AAS, 2013, 44(4 ): 463-467.

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