Spatio-temporal expression of P2X3 receptor in rats with diabetic mechanical allodynia

doi:10.3969/j.issn.0529-1356.2014.04.018

Abstract

Abstract:

Objectiv To investigate the spatio-temporal expression of P2X3 receptor (P2X3R) in rats with diabetic mechanical allodynia (DMA).Methods DMA model in rats was induced by intraperitoneal injection of streptozocin (STZ). The von Frey filaments were applied to identify the changes of the paw withdrawal threshold (PWT) in DMA rats. Immunofluorescence was employed to detect the spatiotemporal expression of P2X3R in the spinal dorsal horn (SDH), dorsal root ganglion (DRG) and hind paw skin on different time points after intraperitoneal injection of STZ, respectively. The protein expression of P2X3R in SDH and DRG was further semi-quantitatively analyzed by Western blotting. Results Compared with control group, intraperitoneal injection STZ induced significant mechanical allodynia indicated by the reduced PWT from 7 days, and which reached the peak on 14d and maintained to 28days (P<0.05). The expression of P2X3R in DRG neurons was significantly increased on 14 days and 21 days (P<0.05), while that in SDH and skin was markedly increased on 21 days and 28 days, compared with control group (P<0.05). Conclusion With the progress of DMA, the expression of P2X3R was significantly increased in the SDH, DRG and skin, which was almost parallel with the mechanical allodynia, but the changes in SDH and skin were 1 week later than that in DRG. These results suggest that P2X3R may play a key role in the maintenance of the DMA.

Key words:

Diabetes mellitus, Mechanical allodynia, P2X3 receptor, Spinal dorsal horn, Dorsal root ganglion, Immunofluorescence, Western blotting, Rat

CUI Yuan-yuan WU Huang-hui WANG Lan SHI Juan LI Yun-qing*. Spatio-temporal expression of P2X3 receptor in rats with diabetic mechanical allodynia[J]. AAS, 2014, 45(4): 540-544.

References

［1］Obrosova IG. Diabetic painful and insensate neuropathy: pathogenesis and potential treatments［J］. Neurotherapeutics, 2009, 6(4): 638-647.
［2］Cameron NE, Jack AM, Cotter MA. Effect of alpha-lipoic acid on vascular responses and nociception in diabetic rats［J］. Free Radic Biol Med, 2001, 31(1): 125-135.
［3］Obrosova IG, Drel VR, Oltman CL, et al. Role of nitrosative stress in early neuropathy and vascular dysfunction in streptozotocin-diabetic rats［J］. Am J Physiol Endocrinol Metab, 2007, 293(6): E1645-1655.
［4］Giniatullin R, Nistri A. Desensitization properties of P2X3 receptors shaping pain signaling［J］. Front Cell Neurosci, 2013, 7: 245.
［5］Wirkner K, Sperlagh BP, Illes P. P2X3 receptor involvement in pain states［J］. Mol Neurobiol, 2007, 36(2): 165-183.
［6］North RA. P2X3 receptors and peripheral pain mechanisms［J］. J Physiol, 2004, 554(Pt 2): 301-308.
［7］Tu WZ, Cheng RD, Cheng B, et al. Analgesic effect of electroacupuncture on chronic neuropathic pain mediated by P2X3 receptors in rat dorsal root ganglion neurons［J］. Neurochem Int, 2012, 60(4): 379-386.
［8］Liu M, Yang H, Fang D, et al. Upregulation of P2X3 receptors by neuronal calcium sensor protein VILIP1 in dorsal root ganglions contributes to the bone cancer pain in rats［J］. Pain, 2013, 154(9): 1551-1568.
［9］Wu JX, Xu MY, Miao XR, et al. Functional up-regulation of P2X3 receptors in dorsal root ganglion in a rat model of bone cancer pain［J］. Eur J Pain, 2012, 16(10): 1378-1388.
［10］Migita K, Moriyama T, Koguchi M, et al. Modulation of P2X receptors in dorsal root ganglion neurons of streptozotocin-induced diabetic neuropathy［J］. Neurosci Lett, 2009, 452(2): 200-203.
［11］Xu GY, Li G, Liu N, et al. Mechanisms underlying purinergic P2X3 receptor-mediated mechanical allodynia induced in diabetic rats［J］. Mol Pain, 2011, 7: 60.
［12］Kamiya H, Zhang W, Ekberg K, et al. C-Peptide reverses nociceptive neuropathy in type 1 diabetes［J］. Diabetes, 2006, 55(12): p. 3581-3587.
［13］Zhang W, Murakawa Y, Wozniak KM, et al. The preventive and therapeutic effects of GCPII (NAALADase) inhibition on painful and sensory diabetic neuropathy［J］. J Neurol Sci, 2006, 247(2): 217-223.
［14］Kim SH, Kwon JK, Kwon YB, et al. Pain modality and spinal glia expression by streptozotocin induced diabetic peripheral neuropathy in rats［J］. Lab Anim Res, 2012, 28(2): 131-136.
［15］Kaan TK, Yip PK, Patel S, et al. Systemic blockade of P2X3 and P2X2/3 receptors attenuates bone cancer pain behaviour in rats［J］. Brain, 2010, 133(9): 2549-2564.
［16］McGaraughty S, Wismer CT, Zhu CZ, et al. Effects of A-317491, a novel and selective P2X3/P2X2/3 receptor antagonist, on neuropathic, inflammatory and chemogenic nociception following intrathecal and intraplantar administration［J］. Br J Pharmacol, 2003, 140(8): 1381-1388.
［17］Tsuda M, Koizumi S, Kita A, et al. Mechanical allodynia caused by intraplantar injection of P2X receptor agonist in rats: involvement of heteromeric P2X2/3 receptor signaling in capsaicin-insensitive primary afferent neurons［J］. J Neurosci, 2000, 20(15): RC90.
［18］Ou S, Zhao YD, Xiao Z, et al. Effect of lappaconitine on neuropathic pain mediated by P2X3 receptor in rat dorsal root ganglion［J］. Neurochem Int, 2011, 58(5): 564-573.
［19］Kage K, Niforatos W, Zhu CZ, et al. Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat［J］. Exp Brain Res, 2002, 147(4): 511-519.
［20］Chen Y, Li GW, Wang C, et al. Mechanisms underlying enhanced P2X receptor-mediated responses in the neuropathic pain state［J］. Pain, 2005, 119(1-3): 38-48.

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