Cadmium damages the blood-testis barrier in rats and the protective effect of Astragaloside IV

doi:10.3969/j.issn.0529-1356.2014.05.021

Abstract

Abstract:

Objective To observe the effect of astragaloside IV (A) and SB203580 antagonize cadmium(Cd) toxicity on expression of associated protein and blood-testis barrier(BTB) in rats and to study the protective mechanism of A on it. Methods Totally 21 SD male rats were randomly divided into 7 groups, 3 rats per group：Cd ［intraperitoneally injected with 0.1%CdCl2，1mg/(kg·d)］，Cd+A ［at the above dose of CdCl2，at the same time with A，10mg/(kg·d)］，and Cd + SB203580 ［at the above dose of CdCl2，at the same time with SB203580，100μg/(kg·d)］， each of the above groups was further divided into continuous five and ten days treatment groups. The control group was intraperitoneally injected with equal dosage of normal saline. The testes were studied by light, electron microscopy, immunohistochemistry and Western blotting. Results In the control group，irregular and lightly stained nuclei of Sertoli cell(Sc) in seminiferous tubules were observed by HE staining. A continuous electron density line of tight junction（TJ）and normal ultrastructure of BTB were observed. After Cd treatment，the vesicular formation in the Sc was observed. The ultrastructural damage of Sc and TJ was observed. Compared with the corresponding time point of Cd group，these were weakened in morphology of testis and ultrastructure of TJ after Cd + A or Cd + SB203580 treatment. The positive products of zonula occludens-1（ZO-1） and claudin-11 were localized mainly in the base of the seminiferous tubule. After Cd treatment，the average absorbance (AA) of ZO-1 and Claudin-11 was decreased significantly compared with that of the control group（P＜0.05）. After Cd + A or Cd + SB203580 treatment，AAof ZO-1 and Claudin-11 were increased significantly compared with that of the Cd group（P＜0.05），though lower than that of the control group. The result of Western blotting showed that phosphorylation-p38MAPK in Cd group was increased significantly compared with that of the control group（P＜0.05）.After Cd + A or Cd + SB203580 treatment, it was decreased significantly compared with that of the Cd group（P＜0.05）. Conclusion Cd decreases ZO-1 and Claudin-11 expression and damages ultrastructure of TJ in BTB, as Ⅳ has protective effect on it, and is related to inhibiting activation of p38 MAPK pathway.

Key words: Cadmium, Astragaloside IV, Zonula occludens-1, Claudin-11, P-p38 MAPK, Immunohistochemistry, Western blotting, Rat

NING Wei LIAO Xiao-gang* WANG Yi YAO Zhi-yong MAO Sheng-yan. Cadmium damages the blood-testis barrier in rats and the protective effect of Astragaloside IV[J]. AAS, 2014, 45(5): 704-709.

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