Abstract:

Objective To analyze the role and clinical significance of hypoxia inducible factor 2α(HIF-2α),CITED2 and PTPRZ1 during the development of papillary thyroid carcinoma (PTC) by detecting the expressions of HIF-2α,CITED2 and PTPRZ1 in the PTC tissues. Methods The expressions of HIF-2α,CITED2 and PTPRZ1 proteins in 50 cases of PTC were detected by Western blotting and immunohistochemical SP method, and the relationships with the clinical pathology characteristics were analyzed.The transcriptional level changes of HIF-2αmRNA,CITED2mRNA and PTPRZ1mRNA were tested by real-time reverse transcription polymerase chain reaction. Results The positive expression rate of HIF-2α CITED2 and PTPRZ1 in PTC tissues were obviously higher than that in thyrvid adenoma(TA) group and normal thyroid(N) group（P＜0.01）and expressions between N and TA were no significant difference （P＞0.05）. The expressions of HIF-2α, CITED2 and PTPRZ1 were unrelated to the age, sex and size of the tumor (P>0.05) of PTC patients, but associated with the lymph node metastasis and pathological stage (P<0.05). The transcriptional expression levels of the HIF-2α mRNA, CITED2 mRNA and PTPRZ1 mRNA in PTC tissues were significantly higher than in TA and normal tissues adjacent to the tumors with statistical significance(P<0.05), but there was no statistical difference between in TA and normal tissues adjacent to the tumors (P>0.05). Conclusion The higher expressions of HIF-2α, CITED2 and PTPRZ1 in PTC have a positive correlation with the lymph node metastasis, size and clinicopathologic stage. HIF-2α may promote the occurrence, development, invasion and metastasis of PTC by the higher expression of CITED2 and PTPRZ1.All of them play an important role in the process of PTC, include the occurrence, development, invasion and metastasis.

Key words: Hypoxia inducible factor 2α, CITED2, PTPRZ1, Papillary thyroid carcinoma, Immunohistochemistry, Western blotting, Human