Therapeutic effect of astragalus polysaccharides on early brain injury after subarachnoid hemorrhage in rats

doi:10.16098/j.issn.0529-1356.2017.05.005

Abstract

Abstract:

Objective To investigate whether astragalus polysaccharide(APS) has a therapeutic effect on early brain injury induced by subarachnoid hemorrhage (SAH) in rats. Methods 120 adult male SD rats were randomly divided into sham operation (sham) group, SAH model group (SAH+NS) and APS group (SAH+APS).After the establishment of the SAH model, each group was injected with the corresponding solution immediately. After 24hours, T2 weighted image (T2 weighted imaging, T2WI) was used to evaluate the brain injury;Nissl stainingmethod was applied to observe neuronal specific structure, morphological changes of austenite; Application of immunohistochemistry and Western blottingmethod to detect apoptosis cleaved Caspase-3 and the Bcl-2, Bax protein expression level.Results Histological staining and T2WI result showed that APS (40 mg /kg) could increase the expression of Bcl-2 (P<0.05), down-regulate the expression of cleaved Caspase-3 and Bax (P<0.05) The result showed that APS (40 mg / kg) could decrease the degree of brain injury (P<0.05). Conclusion Astragalus polysaccharide can inhibit the apoptosis of neurons in hippocampus of SAH rats, and has therapeutic effect on early brain injury.

Key words: Astragalus polysaccharide, Subarachnoid hemorrhage, Hippocampus, Western blotting, Rat

ZHU Shi-jie ZHANG Yan TANG Zhong-sheng FAN Rui-juan LU Ying LUO Ya-fei YAN Jun-hao LIU E. Therapeutic effect of astragalus polysaccharides on early brain injury after subarachnoid hemorrhage in rats[J]. Acta Anatomica Sinica, 2017, 48(5): 526-531.

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