Silencing brainlipid binding protein gene inhibiting the proliferation of rat astrocytes in vitro

doi:10.16098/j.issn.0529-1356.2018.02.001

Abstract

Abstract:

Objective To explore the function of brain-lipid binding protein (BLBP) in cultured rat astrocytes in vitro. Methods After culturing rat astrocytes, BLBP small interference RNA (siRNA) was used to silence the expression of endogenous BLBP. Real-time PCR and Western blotting were applied to detect the level of BLBP and c-Myc; the cells viability was detected by using CCK-8 regent, the proliferated cells were labeled by Ki67, and the cells cycle was investigated by flow cytometry. Results The expressions of BLBP and c-Myc were significantly decreased after treatment of BLBP siRNA. BLBP gene silencing suppressed the cells viability and decreased the proportion of Ki67-positive cells. The result of flow cytometry showed that the insufficient expression of BLBP reduced the cells number of S phase. Conclusion The gene silencing of BLBP inhibits the cells proliferation of astrocytes in vitro. BLBP may regulate the proliferation process of astrocytes.

Key words: Brain-lipid binding protein, Astrocyte, Proliferation, Small interference RNA, Western blotting, Rat

LI Hao-ming YANG Qing-qing PENG Min QIN Jiang-bing HAN Xiao CHENG Xiang JIN Guo-hua. Silencing brainlipid binding protein gene inhibiting the proliferation of rat astrocytes in vitro[J]. Acta Anatomica Sinica, 2018, 49(2): 137-142.

References

［1］ Naohisa T, Tatsuya T, Seiichiro O. Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines［J］. BMC Cancer, 2017, 17(1):192-199.

［2］Matsumata M, Sakayori N, Maekawa M, et al. The effects of Fabp7 and Fabp5 on postnatal hippocampal neurogenesis in the mouse［J］. Stem Cells, 2012, 30(7):1532-1543.

［3］Hotamisligil GS, Bernlohr DA. Metabolic functions of FABPs— mechanisms and therapeutic implications［J］. Nat Rev Endocrinol, 2015, 11(10):592-605.

［4］Li H, Jin G, Qin J, et al. Characterization and identification of Sox2+ radial glia cells derived from rat embryonic cerebral cortex［J］. Histochem Cell Biol, 2011, 136(5):515-526.

［5］Kipp M, Gingele S, Pott F, et al. BLBP-expression in astrocytes during experimental demyelination and in human multiple sclerosis lesions［J］. Brain Behav Immun, 2011, 25(8):1554-1568.

［6］White RE, Mctigue DM, Jakeman LB. Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice［J］. J Comp Neurol, 2010, 518(8):1370-1390.

［7］Zou L, Li H, Jin G, et al. Fimbria-fornix (FF)-transected hippocampal extracts induce the activation of astrocytes in vitro［J］. In Vitro Cell Dev Biol Anim, 2014, 50(3):174-182.

［8］Anthony TE, Mason HA, Gridley T, et al. Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells［J］. Genes Dev, 2005, 19(9):1028-1033.

［9］Qin JB, Jin GH, Li HM, et al. Fimbria fornix transected hippocampal extracts promote proliferation and differentiation of radial glia cells in vitro［J］.Acta Anatomica Sinica, 2011, 42(6):721-725.(in Chinese)

秦建兵, 金国华, 李浩明,等. 切割穹隆海马伞海马提取液促进放射状胶质细胞增殖和向神经元分化［J］. 解剖学报, 2011, 42(6):721-725.

［10］Xu LL, Wang L, Yi X, et al. The change of BLBP expression in rat hippocampus after the fimbria/fornix transection［J］. Chinese Journal of Neuroanatomy, 2008, 24(6):570-574.(in Chinese)

徐璐璐, 王磊, 衣昕,等. 切割穹窿海马伞大鼠海马内BLBP的表达变化［J］. 神经解剖学杂志, 2008, 24(6):570-574.

［11］Chaudhury I, Koepp DM. Recovery from the DNA replication checkpoint［J］. Genes, 2016, 7(12):94-104.

［12］Tarradocastellarnau M, Atauri P, Cascante M. Oncogenic regulation of tumor metabolic reprogramming［J］. Oncotarget, 2015, 7(38):62726-62753.

［13］Willmer T, Peres J, Mowla S, et al. The T-box factor TBX3 is important in S-phase and is regulated by c-Myc and cyclin A-CDK2［J］. Cell Cycle, 2015, 14(19):3173-3183.

［14］Claassen GF, Hann SR. A role for transcriptional repression of p21CIP1 by c-Myc in overcoming transforming growth factor beta-induced cell-cycle arrest［J］. Proc Natl Acad Sci USA, 2000, 97(17):9498-9503.

[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[3]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[4]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[5]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[6]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[7]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[8]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[9]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[10]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[11]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[12]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[13]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[14]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[15]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.