Correlation between genetic polymorphism and hypertensive disorder complicating pregnancy in Qinghai

doi:10.16098/j.issn.0529-1356.2018.03.018

Abstract

Abstract:

Objective To explore the correlation between the polymorphisms in endothelial nitric oxide synthase(eNOS), tumor necrosis super family member 13B(TNFSF13B), hypoxia inducible factor-1α(HIF-1α), vascular endothelial growth factor A(VEGFA) and vascular endothelial growth factor receptor 1(VEGFR1) and susceptibility to hypertensive disorder complicating pregnancy (HDCP) of pregnant women in Qinghai. Methods With the performance of a case-control study, a total of 120 Han patients with HDCP were enrolled as the study group, and 100 healthy pregnant women were included as the control group during the same period. All subjects were from Qinghai province. Sequenom MassARRAY assay was utilized to detect the genotypes of ten clinically relevant polymorphisms of HIF-1 α (RS 11549465, RS 115494657 and RS 2057482), TNFSF13B (RS 16972194), eNOS (RS 2070744), VEGFA (RS 3025029 and RS 2010963) and VEGFR1 (RS 7335588, RS 722503 and RS 12584067). Besides, the differences of single nucleotide polymorphism (SNP) sites were compared between groups subsequently. Results eNOS gene, VEGFA gene,and VEGFR1 gene were found to be significantly different between the study group and the normal group (P<0.001 or P<0.05).The proportions of genotype CC in RS 2070744 and RS 3025039 were higher in the study group than those in the control group (all P<0.001). Among them, the odd ratio (OR) of C allele in the two loci were 2.13 (1.45-3.12) and 4.95 (2.97-8.26) in the HDCP population (all P<0.001), respectively.The proportions of genotype GG in RS 2010963 and RS 7335588 were lower in the study group than those in control group (all P<0.05). Meanwhile, the OR of G alleles in the two loci were 0.50 (0.34-0.74) and 0.46 (0.30-0.72) in the HDCP population (all P<0.001), respectively. Furthermore, the proportion of RS 722503 genotype TC in the study group was 56.67%, higher than that of the control group of 29%, and the OR of C allele was 2.46 (1.58-3.84) in the population HDCP (P<0.001). Conclusion The genetic polymorphisms of VEGFA, VEGFR1 and eNOS are associated with HDCP in Qinghai province, which may be the potential genetic risk factors inducing high incidence of the disease in Qinghai.

Key words: Qinghai, Genetic polymorphism, Hypertensive disorder complicating pregnancy, Time-of-flight mass spectrometry, Female

WANG Xiang-lin ZHAO De-xiong ZHUANG Wen-ting LI Hong-rong LI Jian-hua DAI Dong-fang. Correlation between genetic polymorphism and hypertensive disorder complicating pregnancy in Qinghai[J]. Acta Anatomica Sinica, 2018, 49(3): 379-386.

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