Effects of intervention of brain lipid binding protein expression on differentiation of neural stem cells

doi:10.16098/j.issn.0529-1356.2018.04.002

Abstract

Abstract:

Objective To explore the effect of BLBP on differentiation of hippocampal neural stem cells (NSCs) into neurons in vitro. Methods BLBP overexpression adenovirus and BLBP RNA interference lentiviral vector were built. NSCs were obtained from rat hippocampus by serum-free culturing and immunofluorescence was used to identify NSCs. The NSCs were assigned to Ad-NC group, Ad-BLBP group, LV-NC-RNAi group and LV-BLBP-RNAi group. On the 4th day after infection, BLBP mRNA and protein expression in the NSCs were detected by Real-time PCR and Western blotting, and the neuronal class Ⅲ β-tubulin (Tuj1) positive cells were detected by immunofluorescence. Results NSCs in Ad-BLBP group expressed a high level of BLBP and there were only fewer Tuj1 positive cells than the Ad-NC group; NSCs in LV-BLBP-RNAi group expressed a lower level of BLBP and there were more Tuj1 positive cells than the LV-NC-RNAi group. Conclusion Down regulation of BLBP expression can promote the differentiation of NSCs into neurons in vitro.

Key words: Brain lipid binding protein, Neural stem cells, Differentiation, Neurons, Western blotting, Rat

YU Ting-ting LI Hao-ming JIN Guo-hua HAN Xiao QIN Jian-bing. Effects of intervention of brain lipid binding protein expression on differentiation of neural stem cells[J]. Acta Anatomica Sinica, 2018, 49(4): 424-430.

References

［1］ Carlson SE. Docosahexaenoic acid supplementation in pregnancy and lactation［J］. Am J Clin Nutr, 2009, 89(2):678S-684S.

［2］ Hoo RL, Lee IP, Zhou M, et al. Pharmacological inhibition of adipocyte fatty acid binding protein alleviates both acute liver injury and nonalcoholic steatohepatitis in mice［J］. J Hepatol, 2013, 58(2):358-364.

［3］ Shimizu F, Watanabe TK, Shinomiya H, et al. Isolation and expression of a cDNA for human brain fatty acid-binding protein_B-FABP［J］. Bochim Biophys Acta,1997, 1354(1): 24-28.

［4］ Watanabe A,Toyota T,Owada Y,et al. Fabp7 maps to a quantitative trait locus for a schizophrenia endophenotype［J］. PLoS Biol, 2007, 5(11):e297.

［5］ Liu ShY, Zhang KCh, Yang H, et al. Isolation, culture and indentification of neural stem cells［J］. Journal of Third Military Medical University, 2000, 22(1): 26-28. (in Chinese)

刘仕勇, 张可成, 杨辉,等. 神经干细胞的分离培养及其鉴定［J］. 第三军医大学学报, 2000, 22(1):26-28.

［6］ Oeemig JS, Jrgensen ML, Hansen MS, et al. Backbone and sidechain 1H, 13C and 15N resonance assignments of the human brain-type fatty acid binding protein (FABP7) in its apo form and the holo forms binding to DHA, oleic acid, linoleic acid and elaidic acid［J］. Biomol Nmr Assig, 2009, 3(1):89-93.

［7］ Giachino C, Taylor V. Lineage analysis of quiescent regenerative stem cells in the adult brain by geneticlabeling reveals spatially restricted neurogenic niches in the olfactory bulb［J］.Eur J Neurosci, 2009, 30(1):9-24.

［8］ Bjornsson CS, Apostolopoulou M, Tian Y, et al. It takes a village: constructing the neurogenic niche［J］. Dev Cell, 2015, 32(4):435-446.

［9］ Bátiz LF, Castro MA, Burgos PV, et al. Exosomes as novel regulators of adult neurogenic Niches［J］. Front Cell Neurosci, 2016, 9:501.

［10］ Stipcevic T, Knight CP, Kippin TE. Erratum to: stimulation of adult neural stem cells with a novel glycolipid biosurfactant［J］. Acta Neurol Belg, 2013, 113(4):501-506.

［11］ Giachino C, Taylor V. Lineage analysis of quiescent regenerative stem cells in the adult brain by genetic labelling reveals spatially restricted neurogenic niches in the olfactory bulb［J］. Eur J Neurosci,2009, 30(1):9-24.

［12］ Imayoshi I, Sakamoto M, Yamaguchi M, et al. Essential roles of Notch signaling in maintenance of neural stem cells in developing and adult brains［J］. J Neurosci, 2010, 30(9):3489-3498.

［13］ Fuentealba LC, Obernier K, Alvarez-Buylla A. Adult neural stem cells bridge their niche［J］. Cell Stem Cell,2012,10(6):698-708.

［14］ Beckervordersandforth R, Tripathi P, Ninkovic J, et al. In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells ［J］. Cell Stem Cell, 2011, 7(6):744-758.

［15］ Ferrón SR, Charalambous M, Radford E, et al. Postnatal loss of Dlk1 imprinting in stem cells and niche astrocytes regulates neurogenesis ［J］. Nature, 2011, 475(7356):381-385.

［16］ Giachino C, Basak O, Lugert S, et al. Molecular diversity subdivides the adult forebrain neural stem cell population.［J］. Stem Cells, 2014, 32(1):70-84.

［17］ Li XH, Jian XH, Dai YQ, et al. The effects of reactive astrocyte induced by LPS on neuron in the hippocampus［J］. Progress in Modern Biomedicine, 2007, 7(4): 504-506. (in Chinese)

李小红, 简晓红, 代玉桥,等. LPS诱导海马星形胶质细胞活化与活化的星形胶质细胞对海马神经元的影响［J］. 现代生物医学进展, 2007, 7(4):504-506.

［18］ Storch J, Thumser AE. Tissue-specific functions in the fatty acid-binding protein family［J］. J Biol Chem, 2010, 285(43):32679-32683.

［19］ Konantz M, Alghisi E, Müller JS, et al. Evi1 regulates Notch activation to induce zebrafish hematopoietic stem cell emergence［J］. EMBO J, 2016, 35(21):2315-2331.

［20］ Braune EB, Lendahl U. Notch — a goldilocks signaling pathway in disease and cancer therapy［J］. Discov Med, 2016, 21(115):189-196.

［21］ Caraballomiralles V, Cardonarossinyol A, Garcera A, et al. Notch signaling pathway is activated in motoneurons of spinal muscular atrophy［J］. Int J Mol Sci, 2013, 14(6):11424-11437.

［22］ Zhou J, Deng Z, Chen Y, et al. Overexpression of FABP7 promotes cell growth and predicts poor prognosis of clear cell renal cell carcinoma［J］. Urol Oncol, 2015, 33(3):113.

［23］ Li H, Chang YW, Mohan K, et al. Activated Notch1 maintains the phenotype of radial glial cells and promotes their adhesion to laminin by upregulating nidogen［J］. Glia, 2008, 56(6): 646-658.

［24］ Matsumata M, Sakayori N, Maekawa M, et al. The effects of Fabp7 and Fabp5 on postnatal hippocampal neurogenesis in the mouse ［J］. Stem Cells, 2012, 30(7):1532-1543.

［25］ Basak O, Giachino C, Fiorini E, et al. Neurogenic subventricular zone stem/progenitor cells are Notch1-dependent in their active but not quiescent state［J］. J Neurosci, 2012, 32(16):5654-5666.

［26］ Storch J, Thumser AE. Tissue-specific functions in the fatty acid-binding protein family ［J］. J Biol Chem, 2010, 285(43):32679-32683.

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