Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

doi:10.16098/j.issn.0529-1356.2019.01.022

Abstract

Abstract:

Objective To establish an animal model of restenosis in diabetic lower extremity arterial disease rat after percutaneous transluminal angioplasty(PTA). Methods We divided diabetic rats(n=30) into three groups: atherosclerosis group(AS), restenosis group(RS) and control group. We examined the artery by hematoxylin and eosin(HE), and detected the plaque components by αsmooth muscle actin(α-SMA) immunohistochemical staining. Results Artery restenosis rat models were successfully developed. The smooth muscle cells (SMCs) were the key components of the RS plaque. Conclusion An experimental animal model of restenosis in diabetic lower extremity arterial disease rat after PTA is set up by the steps described in this study.

Key words: Diabetes, Restenosis, Vascular smooth muscle cell, Percutaneous transluminal angioplasty, HE staining, Immunohistochemistry, Rat

ZOU Zhi-wei ZHANG Qian DONG Jian-jun. Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty[J]. Acta Anatomica Sinica, 2019, 50(1): 128-131.

References

［1］ Matsuzaki K, Hayashi R, Okabe K, et al. Prognosis of critical limb ischemia: Major vs minor amputation comparison［J］.Wound Repain Regen,2015,23(5):759-764.

［2］ Aiello A, Anichini R, Brocco E, et al. Treatment of peripheral arterial disease in diabetes: a consensus of the Italian Societies of Diabetes (SID, AMD), Radiology (SIRM) and Vascular Endovascular Surgery (SICVE)［J］.Nutr Metab Cardiovasc Dis,2014,24(4):355-369.

［3］ S?derstr?m MI, Arvela EM, Korhonen M, et al. Infrapopliteal Percutaneous transluminal angioplasty versus bypass surgery as firstline strategies in criticallegation: a propensity score analysis ［J］. Ann Surg, 2010,252 (5): 765-773.

［4］ Liistro F, Porto Ⅰ, Angioli P, et al. Drug-eluting balloon in peripheral intervention for below the knee angioplasty evaluation (DEBATE-BTK): a randomized trial in diabetic patients with critical limb ischemia［J］. Circulation, 2013, 128(6):615-621.

［5］ Schillinger M, Exner M, Mlekusch W, et al. Vascular inflammation and percutaneous transluminal angioplasty of the femoropopliteal artery: association with restenosis［J］. Radiology, 2002, 225(1): 21-26.

［6］ Jamsen T, Manninen H, Tulla H, et al. The final outcome of primary infrainguinal percutaneous transluminal angioplasty in 100 consecutive patients with chronic critical limb ischemia［J］. J Vasc Interv Radio, 2002, 13(5): 455-463.

［7］ Fang D, Lin HSh, Yu F, et al. Anatomy of the superficial epigastric artery perforator flap in rats［J］. Acta Anatomica Sinica, 2014,45(4):531-535. (in Chinese)

方冬,林海双,余丰,等.大鼠腹壁浅动脉穿支皮瓣的解剖学［J］.解剖学报,2014,45(4):531-535.

［8］ Peng XW, Huang J, Yan HF, et al. A rat model of vascular restenosis after thoracoabdominal aortic injury: an experimental study［J］. Hunan Journal of Traditional Chinses Medicine, 2017, 33(5):150-152. (in Chinese)

彭熙炜,黄娟,阎卉芳,等.建立大鼠胸腹主动脉损伤后血管再狭窄模型的实验研究［J］.湖南中医杂志,2017,33(5):150-152.

［9］ Zhou X, Dong J, Zhang L, et al. Hyperglycemia has no effect on development of restenosis after percutaneous transluminal angioplasty (PTA) in a diabetic rabbit model［J］. Endocrinol, 2015, 224(2):119-125.

［10］Edlin RS, Tsai S, Yamanouchi D, et al. Characterization of primary and restenotic atherosclerotic plaque from the superficial femoral artery: potential role of Smad3 in regulation of SMC proliferation［J］. J Vasc Surg, 2009, 49(5): 1289-1295.

[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[3]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[4]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[5]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[6]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[7]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[8]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[9]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[10]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[11]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[12]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.
[13]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[14]	YAO Su-hua YAN Jun XIE Fang LIN Chun-hua HUANG Zheng-chun. Nerve repair effect of olanzapine-mediated cyclic AMP response element binding protein/brain-derived neurotrophic factor/receptor tyrosine kinase receptors B pathway on schizophrenic rats [J]. Acta Anatomica Sinica, 2023, 54(6): 660-667.
[15]	XU Ying LIU Bin ZHENG Xing HE Zong-zhao. Role and mechanism of complement C3a receptor in the cognitive impairment of septic rats induced by cecal ligation and perforation [J]. Acta Anatomica Sinica, 2023, 54(6): 668-675.