Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (3): 346-350.doi: 10.16098/j.issn.0529-1356.2019.03.014

• Histology,Embryology and Developmental Biology • Previous Articles     Next Articles

Expression of RTCB in different tissues of mice

HAO Hai-xia WANG Fu-long AI Gui-qing LI Ran ZHAO Na HE Cheng-qiang DING Nai-zheng*   

  1. College of Life Sciences, Shandong Normal University, Ji’nan 250014, China
  • Received:2018-05-04 Revised:2018-11-08 Online:2019-06-06 Published:2019-06-06
  • Contact: DING Nai-zheng E-mail:nzding@126.com

Abstract:

Objective To investigate the expression and distribution of RTCB in various tissues of mice. Methods We detected the expression of RTCB by Western blotting and the location of the expression of RTCB in each tissue by immunohistochemistry. Results RTCB protein was all expressed in 17 tissues of mice. The relative expressions of oviduct, skeletal muscle and kidney were high, while the relative expressions of bladder and small intestine were low. In addition, the immunostaining of RTCB was different in different tissues or in different cells of the same tissue. The immunostaining was stronger in some specific cells of testis, epididymis, ovary, uterus, fallopian tube and small intestine. Conclusion RTCB may participate in the maintenance of reproductive function in mice.

Key words: RTCB, Tissue expression, Western blotting, Immunohistochemistry, Mouse