Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

doi:10.16098/j.issn.0529-1356.2022.06.002

Abstract

Abstract:

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat superior cervical ganglion and the effect of chronic intermittent hypoxia (CIH) on BACE1 level. Methods The expression and distribution of BACE1 in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACE1 and peroxisome proliferators activated receptor gamma co-activator 1 alpha (PGC-1α) mRNA level was detected by RT-PCR.

Results BACE1 was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACE1 mRNA level, but up-regulated PGC-1α mRNA level (P<0.01). Conclusion BACE1 is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACE1 may be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.

Key words: β-site amyloid precursor protein cleaving enzyme 1, Superior cervical ganglion, Chronic intermittent hypoxia, Western blotting, Rat

CLC Number:

FAN Ya-nan LI Chao-hong JIA Xiang-lei JIN Jia-hao LIU Yu-zhen. Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues[J]. Acta Anatomica Sinica, 2022, 53(6): 698-704.

References

［1］Ahmad SS, Kamal MA. Current updates on the regulation of beta-secretase movement as a potential restorative focus for management of Alzheimer’s disease ［J］. Protein Pept Lett, 2019, 26(8):579-587.

［2］Gerald K. BACE1 Function and inhibition: implications of intervention in the amyloid pathway of Alzheimer’s disease pathology ［J］. Molecules, 2017, 22(10):1723-1742.

［3］Salman LA, Shulman R, Cohen JB. Obstructive sleep apnea, hypertension, and cardiovascular risk: epidemiology, pathophysiology, and management ［J］. Curr Cardiol Rep, 2020, 22(2):6-15.

［4］Wszedybyl-Winklewska M, Wolf J, Szarmach A, et al. Central sympathetic nervous system reinforcement in obstructive sleep apnoea［J］. Sleep Med Rev, 2018, 39:143-154.

［5］Kameda Y. Signaling molecules and transcription factors involved in the development of the sympathetic nervous system, with special emphasis on the superior cervical ganglion ［J］. Cell Tissue Res, 2014, 357(3):527-548.

［6］Liu Y, Ji ES, Xiang S, et al. Exposure to cyclic intermittent hypoxia increases expression of functional NMDA receptors in the rat carotid body ［J］. Appl Physiol, 2009, 106(1):259-267.

［7］St-Pierre J, Drori S, Uldry M, et al. Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators［J］. Cell, 2006, 127(2):397-408.

［8］Katsouri L, Parr C, Bogdanovic N, et al. PPARγ co-activator-1α (PGC-1α) reduces amyloid-β generation through a PPARγ-dependent mechanism［J］. Alzheimers Dis, 2011, 25(1):151-162.

［9］Sadleir KR, Kandalepas PC, Buggia-Prévot V, et al. Presynaptic dystrophic neuritis surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer’s disease［J］. Acta Neuropathol, 2016, 132(2):235-256.

［10］Fisette JF, Montagna DR, Mihailescu MR, et al. A G-rich element forms a G-quadruplex and regulates BACE1 mRNA alternative splicing ［J］. J Neurochem, 2012, 121(5):763-773.

［11］Hung CO, Coleman MP. KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons ［J］. Traffic, 2016, 17(11):1155-1167.

［12］Fazliogullari Z, Kilic C, Karabulut AK, et al. A morphometric analysis of the superior cervical ganglion and its surrounding structures ［J］. Surg Radiol Anat, 2016, 38(3):299-302.

［13］Takaki F, Nakamuta N, Kusakabe T, et al. Sympathetic and sensory innervation of small intensely fluorescent (SIF) cells in rat superior cervical ganglion ［J］. Cell Tissue Res, 2015, 359(2):441-451.

［14］Hanani M. Satellite glial cells in sympathetic and parasympathetic ganglia: In search of function ［J］. Brain Res Rev, 2010, 64(2):304-327.

［15］Farah MH, Pan BH, Hoffman PN, et al. Reduced BACE1 activity enhances clearance of myelin debris and regeneration of axons in the injured peripheral nervous system ［J］. Neuroscience, 2011, 31(15):5744-5754.

［16］Prabhakar NR, Dick TE, Nanduri J, et al. Systemic, cellular and molecular analysis of chemoreflex-mediated sympathoexcitation by chronic intermittent hypoxia［J］. Exp Physiol, 2007, 92(1):39-44.

［17］Hu H, Hicks CW, He W, et al. BACE1 modulates myelination in the central and peripheral nervous system ［J］. Nat Neurosci, 2006, 9(12):1520-1525.

［18］Tallon C, Farah MH. Beta secretase activity in peripheral nerve regeneration ［J］. Neural Regen Res, 2017, 12(10):1565-1574.

［19］Lu W, Kang J, Hu K, et al. The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia ［J］. Sleep Breath, 2017, 21(3):667-677.

［20］Irrcher I, Ljubicic V, Hood DA. Interactions between ROS and AMP kinase activity in the regulation of PGC-1alpha transcription in skeletal muscle cells ［J］. Am J Physiol Cell Physiol, 2009, 296(1):C116-C123.

［21］Wang R, Li JJ, Diao S, et al. Metabolic stress modulates Alzheimer’s β-secretase gene transcription via SIRT1-PPARγ-PGC-1 in neurons ［J］. Cell Metab, 2013, 17(5):685-694.

［22］Gong B, Pan Y, Vempati P, et al. Nicotinamide riboside restores cognition through an upregulation of proliferator-activated receptor-γ coactivator 1α regulated β-secretase 1 degradation and mitochondrial gene expression in Alzheime’s mouse models［J］. Neurobiol Aging, 2013, 34(6):1581-1588.

［23］Christensen MA, Zhou W, Qing H, et al. Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by SP1［J］. Mol Cell Biol, 2004, 24(2):865-874.

［24］Lu W, Ma YY, Shao QQ, et al. ROS/p53/miR-335-5p/Sp1 axis modulates the migration and epithelial to mesenchymal transition of JEG-3 cells［J］. Mol Med Rep, 2020, 21(3):1208-1216.

[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[3]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[4]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[5]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[6]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[7]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[8]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[9]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[10]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[11]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[12]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[13]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[14]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[15]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.