Abstract: Objective To investigate the influences of interferon-γ(IFN-γ) on the intercellular cell adhesion molecule-1(ICAM-1) and IFN-γRα expression in the cultured rat intestinal mucosa microvascular endothelial cells (RIMMVECs). Methods After the RIMMVECs separation culture, IFN-γ in different doses were used to induce the EM>in vitro/EM> cultured RIMMVECs, ICAM-1 and IFN-γRα expression in the cultured RIMMVECs at both the mRNA and protein levels which were detected by realtime PCR and flow cytometry, respectively. Results The results showed that RIMMVECs were activated by IFN-γ at the concentrations of 20μg/L and 40 μg/L. ICAM-1 expression increased in the activated RIMMVECs at both mRNA and protein levels, reaching peak effects at 6 hours; IFN-γRα mRNA expression was increased, but the protein decreased after stimulation by 20 μg/L; IFN-γRα mRNA expression peaked at 2 hours and then decreased after 6 hours when stimulated by

Key words: Intercellular cell adhesion-1, Interferon-γ, Interferon-γ receptor-α, Microvascular endothelial cell, Flow cytometry, Rat