解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (1): 81-84.doi: 10.16098/j.issn.0529-1356.2015.01.014

• 肿瘤生物学 • 上一篇    下一篇

环氧合酶2抑制剂的抗胰腺癌作用及相关机制

谷倬宇1,2 李军1,2* 李思源2* 肖智伟2 周婷3   

  1. 1. 石河子大学医学院第一附属医院内分泌代谢科; 2. 新疆地方与民族高发病教育部重点实验室; 3. 第一附属医院中心实验室，新疆 石河子 832002
  • 收稿日期:2014-09-01 修回日期:2014-10-13 出版日期:2015-02-06 发布日期:2015-02-06
  • 通讯作者: 李军, 李思源 E-mail:846030498@qq.com
  • 基金资助:

    新疆生产建设兵团国际合作基金资助项目;新疆研究生科研创新项目

Anti-tumor effect and mechanism of cyclooxygenase 2 inhibitor in pancreatic cancer

GU Zhuo-yu 1,2 LI Jun 1,2* LI Si-yuan 2* XIAO Zhi-wei2 ZHOU Ting3   

  1. 1. Department of Endocrinology and Metabolism, the First Affiliated Hospital; 2.Key Laboratory of Ministry of Education, Xinjiang Endemic and Ethnic Diseases; 3. the Central Laboratory, the First Affiliated Hospital, 
    Shihezi University School of Medicine, Xinjiang Shihezi 832002, China
  • Received:2014-09-01 Revised:2014-10-13 Online:2015-02-06 Published:2015-02-06
  • Contact: LI Jun , LI Si-yuan E-mail:846030498@qq.com

摘要：

目的 探讨环氧合酶2（COX-2）抑制剂对胰腺癌细胞增殖、侵袭、迁移能力和对COX-2、基质金属蛋白酶（MMP)-14蛋白表达的影响以及可能的抗胰腺癌机制。
方法 不同浓度的COX-2抑制剂塞来昔布(20、60、100μmol/L)处理胰腺癌细胞后，用MTT比色法检测细胞的增殖能力；用Transwell侵袭实验和划痕实验检测细胞的侵袭能力和迁移能力；ELISA检测MMP-14和COX-2的蛋白表达情况。结果 MTT增殖实验、Transwell侵袭实验、划痕实验分别提示，COX-2抑制剂作用后胰腺癌细胞的增殖、侵袭、迁移能力均以浓度梯度形式下降(P<0.05)；ELISA结果显示，胰腺癌细胞中COX-2和MMP-14的蛋白表达水平相应降低(P<0.05)，两者表达具有显著正相关性(r=0.873，P<0.05）。 结论 COX-2抑制剂可能通过抑制COX-2表达下调MMP-14表达，进而以浓度梯度形式减弱胰腺癌细胞的增殖、侵袭、迁移能力，起到抗胰腺癌作用。

关键词: 胰腺癌, 环氧合酶2抑制剂, 基质金属蛋白酶14, 侵袭, 迁移, 四甲基偶氮唑盐法, 人

Abstract:

Objective To investigate the effect of COX-2 inhibitor on proliferation, migration, invasion and the expression of COX-2, matrix metalloproteinase (MMP)-14 in pancreatic cancer cell and its possible anti-tumor mechanism. Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of COX-2 inhibitor celebrex (20, 60, 100μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, Transwell invasion assay, and scratch assay. The protein expression of COX-2 and MMP-14 was assessed by ELISA. Results The proliferation, invasion and migration capabilities were decreased in a concentration-dependent manner by COX-2 inhibitor (P<0.05). The protein expression of COX-2 and MMP-14 was correspondingly reduced (P<0.05). MMP-14 expression was positively correlated with COX-2 expression (r=0.873，P<0.01). Conclusion COX-2 inhibitor may down-regulate MMP-14 expression via inhibiting COX-2 expression, then attenuating the proliferation, invasion and migration of human pancreatic cancer cell in a concentration-dependent manner, contributing to its anti-tumor effect in pancreatic cancer.

Key words: Pancreatic cancer, Cyclooxygenase 2 inhibitor, Matrix metalloproteinase 14, Invasion, Migration, Methyl thiazolyl tetrazolium method, Human