解剖学报 ›› 2015, Vol. 46 ›› Issue (2): 175-181.doi: 10.16098/j.issn.0529-1356.2015.02.006

• 神经生物学 • 上一篇    下一篇

何首乌饮减轻β-淀粉样蛋白诱导的海马神经元损伤的作用机制

陈靖博1 王玉娟1 郭胜男1 牛嗣云2* 段斐2*   

  1. 1. 河北大学基础医学院药理学专业; 2. 河北大学基础医学院组织学胚胎学教研室,河北 保定 071000
  • 收稿日期:2014-10-27 修回日期:2014-12-10 出版日期:2015-04-06 发布日期:2015-04-06
  • 通讯作者: 牛嗣云;段斐 E-mail:nsy1688@163.com
  • 基金资助:

    何首乌饮对衰老大鼠生精细胞凋亡差异表达基因的调节作用;基于信号通路网络节点研究何首乌饮干预睾丸组织退行性病变的分子机制

Effect and mechanism of Heshowuyin on alleviating the hippocampal neurons injury induced by β-amyloid protein

CHEN Jing-bo1 WANG Yu-juan1 GUO Sheng-nan1 NIU Si-yun 2* DUAN Fei 2*   

  1. 1. Pharmacology,School of Basic Medicine, Hebei University; 2. Department of Histology and Embryology,School of Basic Medicine, Hebei University,Hebei Baoding 071000,China
  • Received:2014-10-27 Revised:2014-12-10 Online:2015-04-06 Published:2015-04-06
  • Contact: NIU Si-yun;DUAN Fei E-mail:nsy1688@163.com

摘要:

目的 探讨何首乌饮对β淀粉样蛋白(Aβ)诱导的大鼠原代培养海马神经元损伤保护作用及可能机制。方法 采用新生24h 内的SD大鼠进行原代培养海马神经元,将培养10d的海马神经元分为模型组、何首乌饮保护组、何首乌饮治疗组、何首乌饮对照组及空白对照组。Hoechst33258染色观察海马神经元的凋亡率;免疫组织化学、Western blotting和RT-PCR检测各组海马神经元硫氧还蛋白1(Trx1)蛋白和mRNA的表达情况。 结果 模型组细胞凋亡率增高(P<0.05),Trx1蛋白和mRNA的表达量明显减少(P<0.05);与模型组相比,何首乌饮治疗组和预防组的凋亡率明显降低(P<0.05),Trx1蛋白和mRNA的表达量明显增加(P<0.05)。 结论 何首乌饮可明显减轻Aβ诱导的海马神经元的损伤,其机制可能与增加抗氧化蛋白Trx1的表达有关。

关键词: 海马神经元, 阿尔茨海默病, β-淀粉样蛋白, 何首乌饮, 硫氧还蛋白, 免疫组织化学, 大鼠

Abstract:

Objective To investigate the protective effect of Heshouwuyin on primary hippocampal neuronal cells induced by β-amyloid protein(Aβ)and its possible mechanisms. Methods Hippocampal neurons collected were from newborn SD rats aged in 24 hours.Hippocampal cells which were cultured 10 days were divided into five groups:Model group,Heshouwuyin prevention group,Heshouwuyin treatment group,Heshouwuyin control group and Normal control group.Hoechst33258 staining was used to observe the apoptosis rate of the hippocampal cells.Immunohistochemistry staining was used to observe the expression of Trx1.The mRNA expression of Trx1 was detected in the hippocampal neuronal cells of each group with reverse transcription polymerase chain reaction , and the protein expression of Trx1 was detected in the hippocampal neuronal cells with Western blotting. Results The apoptosis rate of hippocampal neuronal cells was significantly increased in the Model group (P<0.05).In the Model group,the expresstion of Trx1 was significantly decreased(P<0.05).Compared with the Model group,the apoptosis rate of hippocampal neuronal cells was significantly decreased in the Heshouwuyin prevention group and Heshouwuyin treatment group(P<0.05).The mRNA and protein expresstions of Trx1 were significantly higher in Heshouwuyin prevention group and Heshouwuyin treatment group than that in the Model group(P<0.05).Conclusion Heshouwuyin can significantly alleviate the Hippocampal neurons injury induced by Aβ. The neuroprotective activities of Heshouwuyin can be related to its ability to increase the Trx1 expression level.

Key words: Hippocampal neuron, Alzheimer’s disease, β-amyloid protein,  , Heshouwuyin,  , Thioredoxin, Immunohistochemistry, Rat