解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (6): 764-771.doi: 10.16098/j.issn.0529-1356.2015.06.007

• 细胞和分子生物学 • 上一篇    下一篇

趋化因子-8对高糖环境下脂肪间充质干细胞迁移能力的影响

张鹏 张晓东 姜杨 孙石柱 张善强 沈雷*   

  1. 齐齐哈尔医学院解剖学教研室，黑龙江 齐齐哈尔 161006
  • 收稿日期:2015-03-31 修回日期:2015-05-21 出版日期:2015-12-06 发布日期:2015-12-06
  • 通讯作者: 沈雷 E-mail:shenleiby@126.com
  • 基金资助:

    黑龙江省教育厅科学技术研究项目面上课题

Effect of chemokine-8 factor on the migration ability of human adipose derived mesenchymal stem cell in high glucose environment

ZHANG Peng ZHANG Xiao-dong JIANG Yang SUN Shi-zhu ZHANG Shan-qiang SHEN Lei*   

  1. Department of Anatomy, Qiqihar Medical College, Heilongjiang Qiqihaer 161006, China
  • Received:2015-03-31 Revised:2015-05-21 Online:2015-12-06 Published:2015-12-06
  • Contact: SHEN Lei E-mail:shenleiby@126.com
  • Supported by:

    This study was supported by the Foundation of Department

摘要：

目的 探讨高糖环境下，趋化因子-8（CXCL-8）对人脂肪间充质干细胞（hADMSCs）迁移能力的影响和机制。方法 建立高糖环境模型；在高糖条件下，建立CXCL-8实验组、Akt抑制剂组和高糖对照组，在正常条件培养的hADMSCs为正常对照组；分别用细胞划痕实验、Transwell细胞小室实验检测CXCL-8对hADMSCs的迁移能力影响，并用Western blotting、ELISA实验检测Akt、信号转导和转录激活因子（STAT3）和血管内皮生长因子（VEGF）等蛋白表达。
结果 相对高糖对照组，CXCL-8实验组hADMSCs细胞划痕面积闭合率和Transwell细胞小室迁移率均增高（P＜0.01）；而Akt抑制剂组hADMSCs细胞划痕面积闭合率或Transwell细胞小室迁移率比CXCL-8实验组皆降低（P＜0.01）；CXCL-8实验组磷酸化Akt、mTOP、STAT3蛋白表达增高，CXCL-8实验组上清液的VEGF、表皮细胞生长因子 (EGF)含量明显增高（P＜0.01）；而Akt抑制剂组hADMSCs分泌能力下降（P＜0.01）。 结论 高糖环境下，CXCL-8通过Akt-STAT3通路促进间充质干细胞（MSCs)旁分泌VEGF等因子，促进MSCs迁移，对招募宿主细胞归巢，促进组织损伤修复具有重要意义。

关键词: 趋化因子-8, 脂肪间充质干细胞, 高血糖环境, 旁分泌, 归巢, 人

Abstract:

Objective To investigate the effect of chemokines (CXCL)-8 factor on the chemotaxis ability of human adipose-derived mesenchymal stem cells (hADMSCs).
Methods The animals under the high glucose environment were divided into CXCL-8 experimental group, Akt inhibitor group and control group of high glucose. The normal control group was the cultural hADMSCs under the normal condition. Cell scratch, Transwells cell chamber experiment were used to check the effect of chemotaxis ability of CXCL-8 on the ADMSCs Western blotting, and ELISA experiment to check the protein expression of Akt, signal transducer and activator of transcription 3 (STAT3) and vascular endothelial growth factor (VEGF). Results Compared with the control group, the closing rate of hADMSCs cell scratch area in CXCL-8 experimental group and migration rate of Transwell cell chamber were increased (P＜0.01). The closing rate of cell scratch area in Akt inhibitor group and migration rate were decreased (P＜0.01). The protein expressions of phosphorylated Akt, mTOP and STAT3 were increased. The VEGF, epidermal growth factor (EGF) of liquid supernatant in CXCL-8 experimental group were increased significantly (P＜0.01); but the secretion ability of Akt inhibitor group was decreased (P＜0.01). Conclusion Under the high glucose environment, the CXCL-8 promoted MSCs paracrine VEGF factors through Akt-STAT3 pathway and promoted migration of MSCs, which may have the important meaning for recruit host cell homing and promote damage repair.

Key words: Chemokines-8, Adipose-derived mesenchymal stem cell, High glucose environment, Paracrine, Homing, Human