何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响

doi:10.16098/j.issn.0529-1356.2017.01.006

解剖学报 ›› 2017, Vol. 48 ›› Issue (1): 30-36.doi: 10.16098/j.issn.0529-1356.2017.01.006

何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响

王建明^1,2孙静¹齐峰³宋庆亮¹ 牛嗣云^1* 回陈红¹ 徐天戎¹ 贺飞¹

1. 河北大学医学院组织学胚胎学教研室，河北保定 071000; 2. 河北民族师范学院人事处，河北承德 067000; 3. 保定市第一中医院呼吸科，河北保定 071000

收稿日期:2016-06-14 修回日期:2016-09-24 出版日期:2017-02-06 发布日期:2017-02-06
通讯作者: 牛嗣云 E-mail:nsy1688@163.com
基金资助:
国家自然科学基金面上项目;河北省自然科学基金资助项目

Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

WANG Jian-ming ^1,2SUN Jing¹QI Feng³ SONG Qing-liang¹NIU Si-yun ^1* HUI Chen-hong¹XU Tian-rong¹ HE Fei¹

1. Department of Histology and Embryology， School of Medicine, Hebei University，Hebei Baoding 071000, China; 2. Department of Personnel, Hebei Normal University for Nationalities, Hebei Chengde 067000, China; 3. Department of Respiration, Baoding First Hospital of Traditional Chinese Medicine, Hebei Baoding 071000，China

Received:2016-06-14 Revised:2016-09-24 Online:2017-02-06 Published:2017-02-06
Contact: NIU Si-yun E-mail:nsy1688@163.com
Supported by:
;Natural Science Foundation of HeBei Provience

摘要/Abstract

摘要：

目的探究何首乌饮影响大鼠睾丸间质（Leydig)细胞类固醇激素合成急性调节蛋白（StAR）和细胞色素P450胆固醇侧链裂解酶（P450scc）蛋白表达的变化。方法选用10～20 d的幼年雄性 Wistar 大鼠，分离、培养Leydig 细胞，然后收集。采用氧化损伤的方法建立Leydig 细胞衰老模型。实验分组：间质细胞衰老模型组，何首乌饮处理组，首乌丸处理组，正常组和何首乌饮对照组。结果衰老模型组Leydig 细胞StAR和P450scc的免疫组织化学染色强度明显低于正常组和何首乌饮对照组（P<0.01)。另一方面，何首乌饮和何首乌丸干预组StAR和P450scc的免疫组织化学染色强度明显高于衰老模型组(P<0.05)，何首乌饮组好于何首乌丸组。结论何首乌饮可提高睾丸Leydig细胞 StAR 和 P450scc 蛋白的表达，提高睾酮的合成能力，从而延缓Leydig 细胞衰老。

关键词: 何首乌饮, 睾丸Leydig 细胞, 免疫组织化学, 免疫印迹法, 类固醇激素合成急性调节蛋白, P450胆固醇侧链裂解酶, 大鼠

Abstract:

Objective To explore the effect of Heshouwuyin on the steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side chain cleavage enzyme(P450scc) protein expression in Leydig cells of rats. Methods Juvenile male Wistar rat,10-20 days old, were used to isolate the Leydig cells and cultured, and then collected them. The oxidative damage technique was used to establish Leydig cell aging model. This study had a Leydig aging model, Heshouwuyin and Shouwu pill treatments, and normal and Heshouwuyin control group. Results The intensities of immunohistichemical staining for StAR and P450scc were significantly lower in the Leydig aging model group than those in the normal group and Heshouwuyin control group (P<0.01), and were significantly higher in the Heshouwuyin and Shouwu pill group than that in the aging model group (P<0.05). Heshouwuyin group was better than Shouwu pill group. Conclusion Heshouwuyin can increase the protein expression of StAR and P450scc in Leydig cells, improve the synthesis of testosterone, thus delaying the senescence of Leydig cells.

Key words: Heshouwuyin, Testicular Leydig cells, Immunohistichemistudy, Western blotting, StAR, P450scc, Rat

王建明孙静齐峰宋庆亮牛嗣云回陈红徐天戎贺飞. 何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响[J]. 解剖学报, 2017, 48(1): 30-36.

WANG Jian-mingSUN Jing QI Feng SONG Qing-liang NIU Si-yun HUI Chen-hong XU Tian-rong HE Fei. Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats[J]. Acta Anatomica Sinica, 2017, 48(1): 30-36.

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何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响

Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

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