解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (1): 37-42.doi: 10.16098/j.issn.0529-1356.2017.01.007

• 细胞和分子生物学 • 上一篇    下一篇

人外周血细胞放射损伤相关基因的生物信息学

许优沁 张庆芬 张超*   

  1. 南方医科大学基础医学院基因工程研究所，广州 50515
  • 收稿日期:2016-07-28 修回日期:2016-09-21 出版日期:2017-02-06 发布日期:2017-02-06
  • 通讯作者: 张超 E-mail:zhangchao1558@126.com
  • 基金资助:

    国家自然科学基金面上项目

Bioinformatic analysis of gene expression of human peripheral blood cells related to radiation injury

XU You-qin ZHANG Qing-fen ZHANG Chao*   

  1. Institute of Genetic Engineering，School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China
  • Received:2016-07-28 Revised:2016-09-21 Online:2017-02-06 Published:2017-02-06
  • Contact: ZHANG Chao E-mail:zhangchao1558@126.com

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摘要：

目的 从基因水平揭示放射前后人外周血细胞的变化。方法 从基因表达数据库（GEO）中下载两组经放射处理后人外周血基因芯片数据，利用Qlucore Omics Explorer 3.0 软件筛选差异表达基因，STRING、DAVID等在线分析工具对差异表达基因进行下一步的生物信息学分析。 结果 共筛选出94个共同差异表达基因，其中共同表达上调31个，共同表达下调11个，这些差异表达基因主要涉及到细胞凋亡的调控，细胞程序性死亡的调控，细胞死亡的调控，细胞周期的调控，DNA损伤应答，胞内信号转导等的分子功能及生物学过程。通过STRING分析，发现泛素C（UBC）、增殖细胞核抗原（PCNA）、鼠双微体基因-2（MDM2）处在核心节点位置，并参与p53通路。 结论 通过生物信息学的方法分析得出UBC、PCNA、MDM2可能成为潜在的防护放射后损伤的靶点。

关键词: 放射损伤, 基因芯片, 生物信息学, 差异表达基因, 数据分析, 人

Abstract:

Objective To investigate genes associated with radiation injury and to reveal human peripheral blood cells change during radiation. Methods The microarray data of radiation induced gene expression in human blood were downloaded from the Gene Expression Omnibus (GEO) database and Qlucore Omics Explorer 3.0 software was used to screen differentially expressed genes. Further analysis of differentially expressed genes was conducted by the on-line tools STRING and DAVID. Results Of 94 differentially expressed genes, 31 genes were of co-overexpression and 11 genes were co-underexpressed. These genes were involved in the biological process and molecular function of regulation of apoptosis, regulation of programmed cell death, regulation of cell death, intracellular signaling cascade, response to DNA damage stimulus and regulation of cell cycle. STRING analysis showed that ubiquitin C(UBC)，proliferating cell nuclear antigen(PCNA)，murine double minute-2(MDM2) had important roles in the protein-protein interaction network, which participates in p53 pathway. Conclusion UBC, PCNA and MDM2 may be potential therapeutic targets of ionizing radiation exposure through the bioinformatics analysis, which needs further study.

Key words: Radiation injury, Gene microarray, Bioinformatics, Differentially expressed genes, Data analysis, Human