解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (1): 54-60.doi: 10.16098/j.issn.0529-1356.2017.01.010

• 肿瘤生物学 • 上一篇    下一篇

Ezrin在浆液性卵巢癌中的表达及临床意义

谢哲1 李兰芳1 罗清雅2 刘毅1 李贵琴1 王耕超3 毛成毅2 易萍1*   

  1. 1. 第三军医大学第三附属医院野战外科研究所妇产科，重庆 400038;2. 第三军医大学第三附属医院野战外科研究所病理科，重庆 400038;3. 中国医学科学院基础医学研究所医学分子生物学国家重点实验室，北京 100005
  • 收稿日期:2016-07-14 修回日期:2016-09-20 出版日期:2017-02-06 发布日期:2017-02-06
  • 通讯作者: 易萍 E-mail:yp_yiping@foxmail.com
  • 基金资助:

    国家自然科学基金

Expression and clinical significance of Ezrin in the serous ovarian cancer

XIE Zhe1 LI Lan-fang1 LUO Qing-ya2 LIU Yi1 LI Gui-qin1 WANG Geng-chao3 MAO Cheng-yi2 YI Ping 1*   

  1. 1. Deptartment Obstetrics and Gynecology, Research Institute of Surgery and Third Affiliated Hospital, Third Military Medical University, Chongqing 400038, China; 2. Deptartment Pathology, Research Institute of Surgery and Third Affiliated Hospital, Third Military Medical University, Chongqing 400038, China;3. Deptartment of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, State Key Laboratory of Medical Molecular Biology, Beijing 100005, China

  • Received:2016-07-14 Revised:2016-09-20 Online:2017-02-06 Published:2017-02-06
  • Contact: YI Ping E-mail:yp_yiping@foxmail.com

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摘要：

目的 通过检测 Ezrin在正常卵巢上皮和浆液性卵巢癌组织中的差异表达，探讨其对浆液性卵巢癌发生发展的影响。 方法 收取浆液性卵巢癌冷冻组织40例，正常卵巢上皮27例，提取总RNA，采用Real-time PCR技术检测Ezrin mRNA在两组样本中的表达差异；选取有完整临床病理资料的浆液性卵巢癌石蜡包埋组织134例，以及27例非卵巢癌病例的卵巢上皮组织，通过免疫组织化学染色检测两组样本中Ezrin蛋白质的表达差异，并应用SPSS 20.0软件分析其与临床病理的相关性。 结果 Ezrin mRNA在新鲜卵巢癌组织中的表达显著低于正常卵巢上皮组织（P<0.05）。Ezrin蛋白在石蜡包埋卵巢癌组织中也相应降低（P<0.05)。Ezrin蛋白质表达水平与年龄、手术满意程度及化疗敏感程度无相关性，与细胞分化、病理分期及大网膜转移显著相关，（P<0.05)。Ezrin表达水平高的浆液性卵巢癌病例的无进展生存期（PFS）值和总生存期（OS）值都明显高于表达水平低的病例（P<0.05)。但Ezrin并不能作为浆液性卵巢癌的独立预后因素。 结论 Ezrin的表达下调与浆液性卵巢癌的发生发展、转移等病理过程相关，其可以作为临床预后的潜在指标。

关键词: Ezrin；浆液性卵巢癌, 大网膜转移, 无进展生存期, 总生存期, 实时定量聚合酶链反应, 人

Abstract:

Objective To investigate the expression feaure of Ezrin in the normal ovarian epithelial tissues and the serous ovarian cancer tissues and evaluate the relationships between Ezrin expression and the clinicopathological characteristics of ovarian cancer patient. Methods The relative Ezrin mRNA- expression was detected by Real-time PCR in 40 specimens of ovarian carcinoma and 27 specimens of normal ovarian epithelial fresh frozen tissues. A total of 134 specimens of paraffin-embeded ovrian cancer tissues and 27 specimens of normal ovarian epithelial tissues were collected,and the Ezrin protein level was examined by immunohistochemistry. The correlation of Ezrin with the clinicopathological characteristics of patients with ovarian cancer was analyzed by SPSS 20.0. Results Both the RNA and protein levels of Ezrin were significantly reduced in ovarian cancer compared to the normal control(P<0.05). Statistical analysis revealed that Ezrin expression had no correlation with age, cytoreduction satisfactory degree and sensitivity of chemotherapy. However there was a significant difference in ezrin protein levels with the disease-free and overall survival (P<0.05). Although ezrin did not serve as an independent predictor for disease progression (HR 1.406, P>0.05) and overall survival (HR 1.260, P>0.05), the expression of Ezrin was negatively correlated with clinical stage,differentiation, and Oentum majus metastasis (P<0.05). Conclusion The down-regulation of Ezrin may play a substantial role in the pathophysiology of serous ovarian cancer. Therefore, it may be used as the potential pre-diagnostic and prognostic marker of ovarian cancer and direct the clinical treatment in the future.

Key words: Ezrin, Serous ovarian cancer, Oentum majus metastasis, Progression free survival, Overal survival, Real-time PCR, Human