解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (3): 280-284.doi: 10.16098/j.issn.0529-1356.2019.03.003

• 神经生物学 • 上一篇    下一篇

移植微囊化嗅鞘细胞对神经病理性疼痛及L4-5段脊髓P2X7受体表达的影响

张文俊1,2 熊梅娟1 周七保1 孙弋1 刘曾旭2*   

  1. 1.井冈山大学基础医学院解剖学教研室，江西 吉安 343000; 2.南昌大学基础医学院解剖学教研室，南昌 330000
  • 收稿日期:2018-05-18 修回日期:2018-08-03 出版日期:2019-06-06 发布日期:2019-06-06
  • 通讯作者: 刘曾旭 E-mail:1965349157@QQ.COM

Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L4-5 spinal cord

ZHANG Wen-jun 1,2 XIONG Mei-juan1 ZHOU Qi-bao1 SUN Yi1 LIU Zeng-xu 2*   

  1. 1.Department of Anatomy, Basic Medical School, Jinggangshan University, Jiangxi Ji’an 343000,China; 2.Department of Anatomy, Basic Medical School, Nanchang University, Nanchang 330000, China
  • Received:2018-05-18 Revised:2018-08-03 Online:2019-06-06 Published:2019-06-06
  • Contact: LIU Zeng-xu E-mail:1965349157@QQ.COM

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摘要：

目的 探讨微囊化嗅鞘细胞移植对坐骨神经损伤引起病理性疼痛及L4-5段脊髓P2X7受体的表达影响。 方法 取1只健康SD大鼠，取其嗅球组织并运用差速贴壁方法，在体外进行嗅鞘细胞培养扩增。取48只健康SD大鼠，随机分成4组,即对照组（control）、慢性压迫性坐骨神经损伤组（CCI）、嗅鞘细胞移植组（OECs）及微囊化嗅鞘细胞移植组（MC-OECs）。术后7 d及14 d，运用行为学方法检测各组大鼠机械缩足反射阈值，运用原位杂交方法检测各组大鼠L4-5段脊髓P2X7受体阳性细胞百分比及平均吸光度表达情况。 结果 术后7 d及14 d，与对照组比较，CCI组大鼠机械缩足反射阈值明显减低，L4-5段脊髓P2X7受体阳性细胞百分比及平均吸光度明显增加（P<0.05）；与CCI组比，OECs组大鼠机械缩足反射阈值明显增高，P2X7受体阳性细胞百分比及平均吸光度明显减低（P<0.05），与OECs组对比，MC-OECs组大鼠机械缩足反射更高。P2X7受体阳性细胞百分比及平均吸光度更低（P<0.05）。 结论 微囊化嗅鞘细胞移植能更好地缓解神经病理性疼痛，减低L4-5段脊髓内P2X7受体的表达水平，修复坐骨神经损伤。

关键词: 嗅鞘细胞, 微囊化, 移植, L4-5段脊髓, P2X7受体, 原位杂交, 大鼠

Abstract:

Objective To investigate the effect of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4-5 spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OECs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method . The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situhybridization were observed in L4-5spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L4-5spinal cord significantly increased（P<0.05）compared with the CCI group, the reflex threshold of mechanical withdrawl of rats in the OECs group was significantly increased（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L4-5 spinal cord significantly decreased compared with the OECs group(P<0.05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L4-5 spinal cord were lower(P<0.05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain better and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.

Key words: Olfactory ensheathing cell, Microencapsulation, Transplantation, L4-5spinal cord, Purine 2X7 receptor, In situ hybridization, Rat