解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (3): 317-323.doi: 10.16098/j.issn.0529-1356.2019.03.009

• 肿瘤生物学 • 上一篇    下一篇

二氢杨梅素对绒毛膜癌细胞增殖和迁移能力的影响

雷赟涛 刘镭 左彦珍 许倩 鲁艳杰 王康 崔莹 李玉红*   

  1. 承德医学院病理学教研室，河北 承德 067000
  • 收稿日期:2018-05-22 修回日期:2018-09-11 出版日期:2019-06-06 发布日期:2019-06-06
  • 通讯作者: 李玉红 E-mail:youngcheer2003@foxmail.com
  • 基金资助:
    河北省高校重点学科建设项目;河北省高校百名优秀创新人才支持计划Ⅲ;河北省教育厅项目

Effect of dihydromyricetin on the proliferation and migration ability in choriocarcinoma JEG-3 and JAR cells

LEI Yun-tao LIU Lei ZUO Yan-zhen XU Qian LU Yan-jie WANG Kang CUI Ying LI Yu-hong*   

  1. Department of Pathology, Chengde Medical University, Hebei Chengde 067000, China
  • Received:2018-05-22 Revised:2018-09-11 Online:2019-06-06 Published:2019-06-06
  • Contact: LI Yu-hong E-mail:youngcheer2003@foxmail.com

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摘要：

目的 探讨二氢杨梅素（DMY）对绒毛膜癌（绒癌）JEG-3及JAR细胞增殖和迁移能力的影响。 方法 MTT法检测不同浓度的二氢杨梅素(0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L)作用一定时间后，对绒癌JEG-3和JAR细胞增殖能力的影响；细胞划痕实验和Transwell法检测不同浓度二氢杨梅素(0 mg/L, 40 mg/L, 60 mg/L, 80 mg/L)分别作用绒癌JEG-3细胞及JAR细胞一定时间后，对其迁移能力的影响；Real-time PCR和Western blotting方法分别检测不同浓度二氢杨梅素(0 mg/L, 40 mg/L, 60 mg/L, 80 mg/L)作用绒癌JEG-3及JAR细胞后，基质金属蛋白酶2（MMP-2）mRNA和蛋白表达水平的影响。 结果 不同浓度二氢杨梅素作用绒癌JEG-3和JAR细胞24 h和48 h后，随着二氢杨梅素浓度增加，对JEG-3和JAR细胞增殖抑制作用增强（P＜0.05）。二氢杨梅素作用绒癌JEG-3及JAR细胞后，显著抑制细胞迁移能力，且具有浓度依赖性（P＜0.05）。不同浓度二氢杨梅素作用JEG-3和JAR细胞后，MMP-2 的mRNA和蛋白表达水平明显下降（P＜0.05）。 结论 二氢杨梅素能够抑制JEG-3及JAR细胞的增殖能力且具有浓度依赖性，同时二氢杨梅素可能通过下调绒癌JEG-3及JAR细胞中MMP-2 mRNA和蛋白的表达，抑制绒癌细胞的侵袭迁移。

关键词: 绒毛膜癌, 二氢杨梅素, 增殖, 迁移, 迁移小室实验, 实时定量聚合酶链反应

Abstract:

Objective To investigate the effect of dihydromyricetin（DMY）on proliferation and migration in choriocarcinoma JEG-3 and JAR cells. Methods JEG-3 and JAR cells were treated with different concentrations (0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) of DMY for 24 hours and 48 hours, and the proliferation was analyzed by methylthio tetrazole (MTT) assay. The effect of DMY on migration was detected by wound healing (after 24 hours) and Transwell assay (after treated JEG-3 for 36 hours and JAR for 24 hours). The expression of matrix metalloproteinase 2 (MMP-2) at mRNA and protein levels with different concentrations of DMY(0 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) were detected by Real-time PCR (after 12 hours,24 hours,36 hours,48 hours)and Western blotting (after treated 36 hours) respectively. Results The proliferation of JEG-3 and JAR cells was inhibited significantly (P＜0.05), after DMY treatment for 24 hours and 48 hours.DMY inhibited the migration of JEG-3 and JAR cells significantly (P＜0.05) with a dose-dependent manner. After JEG-3 and JAR Cells treated with DMY for 36 hours and 48 hours, the expression of MMP-2 mRNA decreased significantly (P＜0.05),there were no significantly changes in DMY treatment with 12 hours and 24 hours. The expression level of MMP-2 protein was inhibited significantly after treatment with DMY for 36 hours (P＜0.05). Conclusion DMY might inhibit the proliferation in choriocarcinoma JEG-3 and JAR cells with a dose-dependent manner. The invasion and migration were inhibited by DMY through downregulation of MMP-2.

Key words: Choriocarcinoma, Dihydromyricetin, Proliferation, Migration, Transwell, Real-time PCR