大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生

doi:10.16098/j.issn.0529-1356.2019.05.001

解剖学报 ›› 2019, Vol. 50 ›› Issue (5): 549-553.doi: 10.16098/j.issn.0529-1356.2019.05.001

• 神经生物学 • 下一篇

大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生

胡启国刘怀存陈玲赵妍王君*

北京大学基础医学院人体解剖学与组织学胚胎学系，北京 100083

收稿日期:2019-03-26 修回日期:2019-04-22 出版日期:2019-10-06 发布日期:2019-12-10
通讯作者: 王君 E-mail:wangjun74008@bjmu.edu.cn
基金资助:
北京市自然科学基金面上项目

Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia

HU Qi-guo LIU Huai-cun CHEN Ling ZHAO Yan WANG Jun*

Department of Anatomy and Histology and Embryology, Peking University School of Basic Medical Sciences, Beijing 100083,China

Received:2019-03-26 Revised:2019-04-22 Online:2019-10-06 Published:2019-12-10
Contact: WANG Jun E-mail:wangjun74008@bjmu.edu.cn

摘要/Abstract

摘要：

目的探讨背根神经节（DRG）神经元中的Nogo-A在炎症性热痛觉敏化过程中对微管的调控作用及其下游分子通路。方法对野生型大鼠(n=12)和Nogo-A敲除大鼠(n=14)足底注射完全弗式佐剂（CFA）制造炎症痛大鼠模型，取两组大鼠DRG组织，采用Western blotting、免疫荧光染色等方法，研究DRG中Nogo-A在炎症热痛觉敏化过程中对于微管的调节作用。结果 Nogo-A基因敲除具有缓解CFA诱导的炎症性热痛觉敏化作用，Nogo-A基因敲除大鼠DRG中的微管聚合减少，微管上游信号分子磷酸化脑衰反应调节蛋白2（p-CRMP2）表达增加。结论 Nogo-A通过增强CRMP2促进微管聚合，促进了炎症热痛觉敏化的发生。

关键词: Nogo-A, 微管, 脑衰反应调节蛋白2, 背根神经节, 炎症痛, 免疫印迹法, 大鼠

Abstract:

Objective To investigate the regulation of microtubules by Nogo-A in the dorsal root ganglia during the inflammatory pain. Methods The ipsilateral paw withdrawal latency（PWL）was measured in wild type rats（WT，n=12) and Nogo-A konck-out (Nogo-A KO) rats (n=14) after complete Freunds adjuvant (CFA) injection. Western blotting and immunofluorescent staining were used to study the expression of microtubules and phosphorylated collapsin response mediator protein 2（p-CRMP2）in the dorsal root ganglion (DRG) of both groups. Results Knock out of Nogo-A in rats had attenuated the CFA-induced inflammatory hyperalgesia. The acetylated tubulin was reduced, and the expression of p-CRMP2 was increased in the DRG of the Nogo-A KO rats. Conclusion Nogo-A is involved in the regulation of inflammatory heat hyperalgesia by promoting the microtubule polymerization via CRMP2 pathway.

Key words: Nogo-A, Microtubule, Collapsin response mediator protein-2, Dorsal root ganglion, Inflammatory pain, Western blotting, Rat

胡启国刘怀存陈玲赵妍王君 . 大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生[J]. 解剖学报, 2019, 50(5): 549-553.

HU Qi-guo LIU Huai-cun CHEN Ling ZHAO Yan WANG Jun. Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia[J]. Acta Anatomica Sinica, 2019, 50(5): 549-553.

参考文献

［1］ Huber AB, Weinmann O, Brosamle C, et al. Patterns of Nogo mRNA and protein expression in the developing and adult rat and after CNS lesions［J］. J Neurosci，2002, 22 (9): 3553-3567.

［2］ Hu F, Liu HC, Su DQ, et al. Nogo-A promotes inflammatory heat hyperalgesia by maintaining TRPV-1 function in the rat dorsal root ganglion neuron［J］. Faseb J,2019, 33 (1): 668-682.

［3］ Freund J, Stern ER, Pisani TM. Isoallergic encephalomyelitis and radiculitis in guinea pigs after one injection of brain and Mycobacteria in water-in-oil emulsion［J］. J Immunol,1947, 57 (2): 179-194.

［4］ Freund J, Lipton MM,Stone SH. Systemic and local anaphylaxis in the albino rat［J］. J Immunol, 1956, 77 (6): 453-461.

［5］ Li L, Yang XJ. Tubulin acetylation: responsible enzymes, biological functions and human diseases［J］. Cell Mol Life Sci, 2015, 72 (22): 4237-4255.

［6］ Khanna R, Wilson SM, Brittain JM, et al. Opening Pandora's jar: a primer on the putative roles of CRMP2 in a panoply of neurodegenerative, sensory and motor neuron, and central disorders［J］. Future Neurol, 2012, 7 (6): 749-771.

［7］ Goswami C, Schmidt H, Hucho F. TRPV1 at nerve endings regulates growth cone morphology and movement through cytoskeleton reorganization［J］. FEBS J, 2007, 274 (3): 760-772.

［8］ Goswami C, Dreger M, Otto H, et al. Rapid disassembly of dynamic microtubules upon activation of the capsaicin receptor TRPV1［J］. J Neurochem, 2006, 96 (1): 254-266.

［9］ Gu Y, Li G, Huang LYM. Inflammation induces Epac-protein kinase C alpha and epsilon signaling in TRPV1-mediated hyperalgesia［J］. Pain, 2018, 159 (11): 2383-2393.

［10］Storti B, Bizzarri R, Cardarelli F, et al. Intact microtubules preserve transient receptor potential vanilloid 1 (TRPV1) functionality through receptor binding［J］. J Biol Chem, 2012, 287 (10): 7803-7811.

［11］Niederost B, Oertle T, Fritsche J, et al. Nogo-A and myelin-associated glycoprotein mediate neurite growth inhibition by antagonistic regulation of RhoA and Rac1［J］. J Neurosci, 2002, 22 (23): 10368-10376.

［12］Nash M, Pribiag H, Fournier AE, et al. Central nervous system regeneration inhibitors and their intracellular substrates［J］. Mol Neurobiol, 2009, 40 (3): 224-235.

［13］Schmandke A, Schmandke A, Strittmatter SM. ROCK and Rho: biochemistry and neuronal functions of Rho-associated protein kinases［J］. Neuroscientist, 2007, 13 (5): 454-469.

［14］Mimura F, Yamagishi S, Arimura N, et al. Myelin-associated glycoprotein inhibits microtubule assembly by a Rho-kinase-dependent mechanism［J］. J Biol Chem, 2006, 281 (23): 15970-15979.

[1]	洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉 . 酒精性肝纤维化核因子κB信号通路与性别的差异 [J]. 解剖学报, 2024, 55(1): 55-61.
[2]	谢惠兰方芳林毅. Chir99021调控Wnt/β-catenin 信号通路抑制大鼠牙髓干细胞成骨诱导分化的机制[J]. 解剖学报, 2024, 55(1): 67-72.
[3]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[4]	袁蕾杨智伟杨惠刘政海何洁万炜. 三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛 [J]. 解剖学报, 2024, 55(1): 25-31.
[5]	马婷婷陈建红刘爱翠李海宁. 抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用 [J]. 解剖学报, 2024, 55(1): 32-42.
[6]	邹成功冯浩陈兵唐辉邵川孙谋杨荣何家全. 创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化 [J]. 解剖学报, 2024, 55(1): 43-48.
[7]	吕海燕沈西雅赵富生朱梅. 松茸多糖对 1-甲基-4-苯基-吡啶离子诱导 PC12 细胞损伤的影响 [J]. 解剖学报, 2024, 55(1): 49-54.
[8]	郑丽平刘霞杜晓华吴亚娟刘珊珊 . 脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布 [J]. 解剖学报, 2024, 55(1): 10-16.
[9]	苏芃王兴仪梁景岩熊天庆. 一种低密度及高纯度胎鼠原代海马神经元培养方法的优化及鉴定[J]. 解剖学报, 2024, 55(1): 113-119.
[10]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[11]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.
[12]	刘丽平朱梦妮徐慧 . 白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响 [J]. 解剖学报, 2023, 54(6): 722-729.
[13]	赵卫明李晓余国营王改平常翠芳 . 丝氨酸肽酶抑制因子Kazal型1对肝癌细胞增殖的影响及其机制 [J]. 解剖学报, 2023, 54(6): 695-702.
[14]	王文娟丁雨桐苏昊任捷孙艳荣王涵菲陆嘉莉张林倩白钰秦丽华. 低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化[J]. 解剖学报, 2023, 54(6): 620-627.
[15]	张琴杨俊霞蒋青松. 福辛普利对糖尿病视网膜病变小鼠血管紧张素转化酶2和血管内皮生长因子的影响[J]. 解剖学报, 2023, 54(6): 628-634.

大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生

Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价