解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (5): 645-650.doi: 10.16098/j.issn.0529-1356.2019.05.017

• 组织学胚胎学发育生物学 • 上一篇    下一篇

脂肪肝对小鼠肝血窦内血流状况的影响

 陈重九1 樊婧1 王宇辰1 全葳1 王建伟1 李石良2 张卫光1*   

  1. 1.北京大学基础医学院人体解剖学与组织学胚胎学系，北京 100191; 2.中日友好医院针灸科，北京 100029
  • 收稿日期:2018-05-07 修回日期:2018-05-15 出版日期:2019-10-06 发布日期:2019-12-10
  • 通讯作者: 张卫光 E-mail:zhangwg@bjmu.edu.cn
  • 基金资助:
    北京市自然基金面上项目

Observing and detecting on blood flow in hepatic sinus in hepatic steatosis mice

CHEN Chong-jiu1 FAN Jing1 WANG Yu-chen1 QUAN Wei1 WANG Jian-wei1 LI Shi-liang2 ZHANG Wei-guang 1*   

  1. 1. Department of Anatomy and Histology and Embryology, Peking University Health Science Center, Beijing 100191, China; 2. Department of Acupuncture, ChinaJapan Friendship Hospital, Beijing 100029, China
  • Received:2018-05-07 Revised:2018-05-15 Online:2019-10-06 Published:2019-12-10
  • Contact: ZHANG Wei-guang E-mail:zhangwg@bjmu.edu.cn

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摘要：

目的 由于脂肪肝状态下肝细胞的体积变化可能导致肝内血液流动特征改变，本研究拟通过观测肝内的形态学、血流速度和迂曲度的变化，为脂肪肝的诊治提供帮助。 方法 小鼠背部皮下注射四氯化碳或橄榄油溶液建立脂肪肝模型，取小鼠肝组织进行HE与油红O染色；采用激光多普勒仪测量肝左叶浅表血管内的血流速度；小鼠尾静脉注射德克萨斯红荧光染料，双光子荧光显微镜下观察及测量小鼠肝血窦内红细胞的流动速度及血管内径状况，并进行肝血窦迂曲度检测。 结果 给予小鼠四氯化碳注射2周（n=16）和4周（n=16）后，油红O染色可见肝细胞内出现不同程度的红色脂滴沉积，且主要集中在中央静脉周围，肝血窦存在明显的变形和缩窄，且4周组更加明显。随着模型制作时间的延长，肝左叶的的浅表区域和肝血窦内的血流速度逐渐降低，且肝血窦的内径变小，迂曲度增加。 结论 脂肪肝状态下，肝血窦内径减小，血窦内血流速度下降，迂曲度增加，为脂肪肝早期诊断和治疗提供了可视化的形态学实验依据。

关键词: 脂肪肝, 血流动力学, 肝血窦, 迂曲度, 双光子荧光显微术, 小鼠

Abstract:

Objective Due to the distortion of hepatic cells in hepatic steatosis, the characteristics of blood flow in the liver could change. This study observed the morphology, blood flow velocity and tortuosity changes aimed to help the diagnosis and treatment in the hepatic steatosis. Methods The hepatic steatosis model was established by subcutaneous injection of carbon tetrachloride (CCl4) and olive oil in mice, and then liver tissue was stained with HE and oil red O staining. Laser ultrasound was used to measure the blood flow changes in the superficial hepatic vessels of the left lobe. The mice’s tail veins were injected with Texas red fluorescent dye, then two-photon fluorescence microscopy were used to detect the flow of red blood cells in mice’s hepatic sinusoids, blood vessel diameter, the hepatic sinusoidal tortuosity. Results After injected with CCl4 for two(n=16) or four(n=16) weeks, the oil red O staining indicated lipid accumulation in hepatic cells, especially around the central vein. HE staining indicated narrowing of the hepatic sinusoidal vessels, and more obviously in 4-weeks group. As the modeling time increased, the blood flow velocity decreased gradually in hepatic sinusoids and superficial hepatic vessels in the left lobe, and the diameter of the hepatic sinusoids became smaller. Conclusion In the hepatic steatosis, the internal diameter of hepatic sinus decreases, and the blood flow also decreases in the hepatic sinusoids, but hepatic sinusoidal tortuosity increases. All of this provide a visual morphological experimental basis for the early diagnosis and treatment to the hepatic steatosis.

Key words: Hepatic steatosis, Hemodynamics, Hepatic sinusoid, Tortuosity, Two-photon fluorescence microscopy, Mouse