小鼠胚胎早期血细胞发生的研究进展

doi:10.16098/j.issn.0529-1356.2019.05.026

解剖学报 ›› 2019, Vol. 50 ›› Issue (5): 698-702.doi: 10.16098/j.issn.0529-1356.2019.05.026

小鼠胚胎早期血细胞发生的研究进展

刘东华¹ 何曦²裴一岩³ 梁雨森³ 李凌衡^2* 刘慧雯^1*

1.哈尔滨医科大学组织学与胚胎学教研室，哈尔滨 150081; 2.Stowers医学研究院，美国密苏里州堪萨斯 64110; 3. 哈尔滨医科大学基础医学院，哈尔滨 150081

收稿日期:2018-07-24 修回日期:2018-09-28 出版日期:2019-10-06 发布日期:2019-12-10
通讯作者: 李凌衡;刘慧雯 E-mail:liuhw_11@yahoo.com
基金资助:
黑龙江省自然科学基金面上项目;黑龙江省卫生计生委科研课题;国家自然科学基金面上项目

Advances in the study of the murine hematopoiesis during the early stage of embryogenesis

LIU Dong-hua¹ HE Xi² PEI Yi-yan³ LIANG Yu-sen³ LI Ling-heng^2* LIU Hui-wen¹

1.Department of Histology and Embryology, Harbin Medical University, Harbin 150081,China; 2.Stowers Institute for Medical Research，KC MO 64110 USA; 3.College of Basic Medicine, Harbin Medical University，Harbin 150081,China

Received:2018-07-24 Revised:2018-09-28 Online:2019-10-06 Published:2019-12-10
Contact: LI Ling-heng;LIU Hui-wen E-mail:liuhw_11@yahoo.com
Supported by:
General project of natural science foundation in Heilongjiang province;Scientific project foundation of Heilongjiang province health and family planing commission;Natural science foundation of China for general project

摘要/Abstract

摘要：

根据时空上的不同，小鼠胚胎发育时期的血细胞发生目前被分为原始造血、红系/髓系祖细胞（EMPs）的产生和造血干细胞（HSCs）成熟并分化为各种血细胞3个阶段。最新的观点也把原始造血和EMPs的产生归结为非HSCs依赖性的血细胞谱系分化阶段，将第3阶段称为HSCs依赖性的血细胞谱系分化阶段。尽管胚胎时期的血细胞发生涉及多个造血器官，但本文中我们主要对近年在细胞和分子水平进行的造血细胞和HSCs在卵黄囊和腹主动脉-性腺-中肾（AGM）区发育的研究进行归纳总结，以此展示新近发现的胚胎发育早期血细胞发生的特点及其潜在机制。

关键词: 血细胞发生, 造血干细胞, 卵黄囊, 腹主动脉-性腺-中肾区, 小鼠胚胎

Abstract:

The development of the murine embryonic hematopoietic system occurs in spatially and temporally distinct waves, which it is described as three waves so far-primitive hematopoiesis, bipotential erythroid-myeloid progenitors (EMPs) generation and long-lived transplantable hematopoietic stem cells (HSCs) maturation from their precursors and differentiation toward all the adult lineages. The latest point is that HSC-independent hematopoietic lineages are produced in the primitive wave and definitive progenitor wave in the early mammalian embryo, such as primitive erythrocytes or EMPs. The HSC-dependent phase of hematopoietic development produces all the adult lineages derived from HSCs. In this review, the recent studies on the development of hematopoietic cells and HSCs in the yolk sac and aorta-gonad-mesonephron region (AGM) region at cellular and molecular level will be summarized to provide an integrated model of developmental hematopoiesis, although multiple hematopoietic sites are involved in ebryonic hematopoiesis. It may offer new insights into the characteristics and its underlying mechanism of hematopoiesis at the early stage of embryogenesis.

Key words: Hematopoiesis, Hematopoietic stem cell, Yolk sac, Aorta-gonad-mesonephron region, Mouse embryo

刘东华何曦2 裴一岩梁雨森李凌衡刘慧雯 . 小鼠胚胎早期血细胞发生的研究进展[J]. 解剖学报, 2019, 50(5): 698-702.

LIU Dong-hua HE Xi PEI Yi-yan LIANG Yu-sen LI Ling-heng LIU Hui-wen. Advances in the study of the murine hematopoiesis during the early stage of embryogenesis[J]. Acta Anatomica Sinica, 2019, 50(5): 698-702.

参考文献

［1］ Kauts ML，Vink CS，Dzierzak E. Hematopoietic (stem) cell development - how divergent are the roads taken［J］? FEBS Lett，2016，590（22）：3975-3986.

［2］ Gritz E，Hirschi KK. Specification and function of hemogenic endothelium during embryogenesis［J］. Cell Mol Life Sci，2016，73（8）：1547-1567.

［3］ Frame JM，Fegan KH，Conway SJ，et al. Definitive hematopoiesis in the yolk sac emerges from Wnt-responsive hemogenic endothelium independently of circulation and arterial identity［J］. Stem Cells， 2016，34（2）：431-444.

［4］ Vo LT，Kinney MA，Liu X，et al. Regulation of embryonic haematopoietic multipotency by EZH1［J］. Nature， 2018，553（7689）：506-510.

［5］ Dzierzak E， Bigas A. Blood development：hematopoietic stem cell dependence and independence［J］. Cell Stem Cell，2018，22（5）：639-651.

［6］ Yumine A，Fraser ST，Sugiyama D. Regulation of the embryonic erythropoietic niche: a future perspective［J］. Blood Res，2017，52（1）：10-17.

［7］ Stefanska M, Batta K, Patel R, et al. Primitive erythrocytes are generated from hemogenic endothelial cells［J］. Sci Rep，2017，7（1）：6401.

［8］ Ratajczak MZ. Why are hematopoietic stem cells so ‘sexy’? on a search for developmental explanation［J］. Leukemia，2017，31（8）：1671-1677.

［9］ Palis J. Hematopoietic stem cell-independent hematopoiesis: emergence of erythroid, megakaryocyte, and myeloid potential in the mammalian embryo［J］. FEBS Lett，2016，590（22）：3965-3974.

［10］Mass E，Ballesteros Ⅰ，Farlik M，et al. Specification of tissue-resident macrophages during organogenesis［J］. Science，2016，353（6304）. pii: aaf4238.

［11］Yzaguirre AD，Howell ED，Li Y，et al. Runx1 is sufficient for blood cell formation from non-hemogenic endothelial cells in vivo only during early embryogenesis［J］. Development，2018，145（2）.pii: dev158162.

［12］Lilly AJ，Costa G，Largeot A，et al. Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac［J］. Development， 2016，143（23）：4341-4351.

［13］Travnickova J，Tran Chau Ⅴ，Julien E，et al. Primitive macrophages control HSPC mobilization and definitive haematopoiesis［J］. Nat Commun，2015，6：6227.

［14］Yoder MC，Hiatt K，Mukherjee P. In vivo repopulating hematopoietic stem cells are present in the murine yolk sac at day 9.0 postcoitus［J］. Proc Natl Acad Sci USA，1997，94（13）:6776-6780.

［15］Huang H，Auerbach R. Identification and characterization of hematopoietic stem cells from the yolk sac of the early mouse embryo［J］. Proc Natl Acad Sci USA，1993，90（21）：10110-10114.

［16］Samokhvalov IM，Samokhvalova NI，Nishikawa S. Cell tracing shows the contribution of the yolk sac to adult haematopoiesis［J］. Nature，2007，446（7139）：1056-1061.

［17］Lee LK，Ghorbanian Y，Wang W，et al. LYVE1 marks the divergence of Yolk Sac definitive hemogenic endothelium from the primitive erythroid lineage［J］. Cell Rep，2016，17（9）：2286-2298.

［18］Migueles RP，Shaw L，Rodrigues NP，et al. Transcriptional regulation of Hhex in hematopoiesis and hematopoietic stem cell ontogeny［J］. Dev Biol，2017，424（2）：236-245.

［19］Rybtsov S，Batsivari A，Bilotkach K，et al. Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor［J］. Stem Cell Reports，2014，3（3）：489-501.

［20］Bergiers Ⅰ，Andrews T，Vargel Bolukbasi O，et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis［J］. Elife，2018，7. pii: e29312.

［21］Hu Y，Huang Y，Yi Y，et al. Single-cell RNA sequencing highlights transcription activity of autophagy-related genes during hematopoietic stem cell formation in mouse embryos［J］. Autophagy，2017，13（4）：770-771.

［22］Souilhol C，Gonneau C，Lendinez JG，et al. Inductive interactions mediated by interplay of asymmetric signalling underlie development of adult haematopoietic stem cells［J］. Nat Commun，2016，7：10784.

［23］Taoudi S，Gonneau C，Moore K, et al. Extensive hematopoietic stem cell generation in the AGM region via maturation of VE-cadherin+CD45+ pre-definitive HSCs［J］. Cell Stem Cell，2008，3（1）：99-108.

［24］Zhou F，Li X，Wang W，et al. Tracing haematopoietic stem cell formation at single-cell resolution［J］. Nature， 2016，533（7604）：487-492.

［25］Taoudi S，Medvinsky A. Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta［J］. Proc Natl Acad Sci USA，2007，104（22）：399-403.

［26 Liu D，He XC，Qian P，et al. Leucine-rich repeat-containing G-protein-coupled receptor 5 marks short-term hematopoietic stem and progenitor cells during mouse embryonic development［J］. J Biol Chem， 2014，289（34）：23809-23816.

［27］Kim PG，Canver MC，Rhee C，et al. Interferon-alpha signaling promotes embryonic HSC maturation［J］. Blood，2016，128（2）：204-216.

［28］Zhang JB. A brief introduction to combined stem cell/gene therapy［J］. Acta Anatomica Sinica, 2012，43（3）：289-292.（in Chinese）

章静波.干细胞/基因联合治疗［J］. 解剖学报，2012，43（3）：289-292.

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小鼠胚胎早期血细胞发生的研究进展

Advances in the study of the murine hematopoiesis during the early stage of embryogenesis

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