三叉神经根慢性压迫损伤对三叉神经节细胞髓鞘碱性蛋白及P75表达的影响

doi:10.16098/j.issn.0529-1356.2019.06.001

解剖学报 ›› 2019, Vol. 50 ›› Issue (6): 707-712.doi: 10.16098/j.issn.0529-1356.2019.06.001

• 神经生物学 • 下一篇

三叉神经根慢性压迫损伤对三叉神经节细胞髓鞘碱性蛋白及P75表达的影响

林仁^1,3 罗丽丽^1,2李秋华^1,2 陈婷^1,2邱荣晖^1,3罗道枢^1,2*

1.福建医科大学基础医学院人体解剖学与组织学胚胎学系; 2.福建省高校脑老化与神经变性疾病重点实验室;3. 福建医科大学基础医学院实验教学中心，福州 350122

收稿日期:2019-03-28 修回日期:2019-07-22 出版日期:2019-12-06 发布日期:2019-12-06
通讯作者: 罗道枢 E-mail:luods2004@163.com
基金资助:
国家自然科学基金面上项目;福建省卫生系统中青年骨干人才培养项目

Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells

LIN Ren^1,3 LUO Li-li ^1,2 LI Qiu-hua ^1,2 CHEN Ting^1,2 QIU Rong-hui ^1,3LUO Dao-shu^1,2*

1.Department of Human Anatomy, Histology and Embryology, School of Basic Medical Science, Fujian Medical University, Fuzhou 350122, China; 2.Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province, Fuzhou 350122, China; 3. Experimental Teaching Center, School of Basic Medical Science, Fujian Medical University, Fuzhou 350122, China

Received:2019-03-28 Revised:2019-07-22 Online:2019-12-06 Published:2019-12-06
Contact: LUO Dao-shu E-mail:luods2004@163.com

摘要/Abstract

摘要：

目的探讨三叉神经节中卫星胶质细胞与神经元相互作用对三叉神经痛（TN）的影响。方法在SD大鼠假手术组（n=24）和三叉神经根慢性压迫诱导的TN大鼠模型组（n=24）中，运用免疫荧光和Western blotting方法，研究髓鞘碱性蛋白（MBP）、胶质纤维酸性蛋白（GFAP）及P75神经营养因子受体（P75NTR）在三叉神经节中的表达情况。结果 TN模型组P75NTR 表达较假手术组增高，术后第7天和第14 天的表达增高有统计学意义（P<0.01）；MBP在TN模型组的表达较假手术组均降低，特别在第7和14天的表达降低有统计学意义（P<0.05）；卫星胶质细胞标志物GFAP在TN模型组术后第7、14和21天表达增高（P<0.01）。结论大鼠三叉神经根区慢性压迫损伤引起三叉神经节神经元MBP和P75NTR 表达改变和卫星胶质细胞的活化，可能影响神经元-卫星胶质细胞的相互作用，参与TN大鼠口面部周围伤害性信息向中枢的传递。

关键词: 三叉神经痛, 三叉神经节, 卫星胶质细胞, 动物模型, 免疫印迹法, 免疫荧光, 大鼠

Abstract:

Objective To investigate the effect of the interaction between satellite glial cells and neurons in the trigeminal ganglion of trigeminal neuralgia (TN) rat model.Methods In the TN rat model (n=24) induced by trigeminal root entry zone chronic compression and sham group (n=24), immunofluorescence and Western blotting were used to detect the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP) and of P75 neurotrophic factor receptor (P75NTR) in the trigeminal ganglion. Results The expression of P75NTR in TN group was higher than that in the sham operation group, especially on the post-operation day (POD) 7 and 14 (P<0.01). The expression of MBP in the TN group was decreased after operation, which were significant lower than that in the sham operation group on POD 7 and 14(P<0.05). While the expression of immunoreactive GFAP in the satellite glial cells in the TN group was higher than that in the sham operation group from POD 7 to POD 21 after operation (P<0.01). Conclusion The changes of MBP and P75NTR expression in neurons and the activation of satellite glial cells in trigeminal ganglion of the TN animal model may affect the interaction of neuron-satellite glial cells, which may be involved in the nociceptive information transmission from orofacial area to the central nervous system in the TN rats.

Key words: Trigeminal neuralgia, Trigeminal ganglion, Satellite glial cell, Animal model, Western blotting, Immunofluorescence, Rat

林仁罗丽丽李秋华陈婷邱荣晖罗道枢. 三叉神经根慢性压迫损伤对三叉神经节细胞髓鞘碱性蛋白及P75表达的影响[J]. 解剖学报, 2019, 50(6): 707-712.

LIN Ren LUO Li-li LI Qiu-hua CHEN Ting QIU Rong-hui LUO Dao-shu. Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells[J]. Acta Anatomica Sinica, 2019, 50(6): 707-712.

参考文献

［1］ Majeed MH, Arooj S, Khokhar MA, et al. Trigeminal neuralgia: a clinical review for the general physician［J］. Cureus, 2018, 10(12): e3750.

［2］ Maarbjerg S, Di Stefano G, Bendtsen L, et al. Trigeminal neuralgia - diagnosis and treatment［J］. Cephalalgia, 2017, 37(7): 648-657.

［3］ Hossain MZ, Unno S, Ando H, et al. Neuron-glia crosstalk and neuropathic pain: involvement in the modulation of motor activity in the orofacial region［J］. Int J Mol Sci, 2017, 18(10): 2051.

［4］ Pannese E, Procacci P. Ultrastructural localization of NGF receptors in satellite cells of the rat spinal ganglia［J］. J Neurocytol, 2002, 31(8-9): 755-763.

［5］ Zhou XF, Rush RA, Mclachlan EM. Differential expression of the p75 nerve growth factor receptor in glia and neurons of the rat dorsal root ganglia after peripheral nerve transection［J］. J Neurosci, 1996, 16(9): 2901-2911.

［6］ Tomita K, Kubo T, Matsuda K, et al. The neurotrophin receptor p75NTR in Schwann cells is implicated in remyelination and motor recovery after peripheral nerve injury［J］. Glia, 2007, 55(11): 1199-1208.

［7］ Zhang H, Ren NT, Zhou FQ, et al. Effects of hindlimb unweighting on MBP and GDNF expression and morphology in rat dorsal root ganglia neurons［J］. Neurochem Res, 2016, 41(9): 2433-2442.

［8］ Luo DS, Zhang T, Zuo CX, et al. An animal model for trigeminal neuralgia by compression of the trigeminal nerve root［J］. Pain Physician, 2012, 15(2): 187-196.

［9］ Pannese E. Biology and pathology of perineuronal satellite cells in sensory ganglia［M］. Germany: Heidelberg, 2018: 1-63.

［10］Chiang CY, Dostrovsky JO, Iwata K, et al. Role of glia in orofacial pain［J］. Neuroscientist, 2011, 17(3): 303-320.

［11］Katagiri A, Shinoda M, Honda K, et al. Satellite glial cell P2Y12 receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats［J］. Mol Pain, 2012, 8(23): 1-8.

［12］Xu M, Aita M, Chavkin C. Partial infraorbital nerve ligation as a model of trigeminal nerve injury in the mouse: behavioral, neural, and glial reactions［J］. J Pain, 2008, 9(11): 1036-1048.

［13］Lin R, Luo L, Gong Y, et al. Immunohistochemical analysis of histone H3 acetylation in the trigeminal root entry zone in an animal model of trigeminal neuralgia［J］. J Neurosurg, 2018, 11(1): 1-11.

［14］Costa FA, Moreira Neto FL. ［Satellite glial cells in sensory ganglia: its role in pain］［J］. Rev Bras Anestesiol, 2015, 65(1): 73-81.

［15］Vassall KA, Bamm VV, Harauz G. MyelStones: the executive roles of myelin basic protein in myelin assembly and destabilization in multiple sclerosis［J］. Biochem J, 2015, 472(1): 17-32.

［16］Ma HY, Yu B, Kong L,et al. Brain derived newotrophic factor gene-modified cortical neural stem cells of rat embryo and their effect on differentiation in vitro［J］. Acat Anatomica Sinica, 2011, 42(1): 70-74.（in Chinese）

马海英，喻博，孔力，等. 脑源性神经营养因子基因修饰的胚胎大鼠皮质神经干细胞及其在体外的分化［J］. 解剖学报, 2011, 42(1): 70-74.

［17］Cosgaya JM, Chan JR, Shooter EM. The neurotrophin receptor p75NTR as a positive modulator of myelination［J］. Science, 2002, 298(5596): 1245-1248.

［18］Saxena S, Howe CL, Cosgaya JM, et al. Differences in the surface binding and endocytosis of neurotrophins by p75NTR［J］. Mol Cell Neurosci, 2004, 26(2): 292-307.

［19］Fukui Y, Ohtori S, Yamashita M, et al. Low affinity NGF receptor (p75 neurotrophin receptor) inhibitory antibody reduces pain behavior and CGRP expression in DRG in the mouse sciatic nerve crush model［J］. J Orthop Res, 2010, 28(3): 279-283.

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三叉神经根慢性压迫损伤对三叉神经节细胞髓鞘碱性蛋白及P75表达的影响

Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells

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