解剖学报

解剖学报 ›› 2020, Vol. 51 ›› Issue (4): 553-556.doi: 10.16098/j.issn.0529-1356.2020.04.013

• 肿瘤生物学 • 上一篇    下一篇

MiR-145-5p在大肠癌中的表达及与多药耐药的关系

袁亚男 杜梦楠 严晓丽 黄艳洁 郑纪宁*   

  1. 承德医学院病理学与病理生理学教研室，河北 承德 067000
  • 收稿日期:2019-03-07 修回日期:2020-04-27 出版日期:2020-08-06 发布日期:2020-08-06
  • 通讯作者: 郑纪宁 E-mail:910235882@qq.com
  • 基金资助:
    承德医学院自然科学研究计划项目;河北省高校省级重点学科建设项目

Expression of miR-145-5p in colorectal cancer and its relationship with multidrug resistance

YUAN Ya-nan DU Meng-nan YAN Xiao-li HUANG Yan-jie ZHENG Ji-ning*#br#   

  1. Department of Pathology and Pathophysiology，Chengde Medical University，Hebei Chengde 067000，China
  • Received:2019-03-07 Revised:2020-04-27 Online:2020-08-06 Published:2020-08-06
  • Contact: ZHENG Ji-ning E-mail:910235882@qq.com

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摘要：

目的 探讨大肠癌中miR-145-5p和多药耐药基因(MDR1)蛋白P-糖蛋白（P-gp）的表达及两者的关系。 方法 分别在组织、细胞水平采用SP法、Real-time PCR法、Western blotting法检测50例大肠癌 (CRC)患者组织及30例癌旁正常组织患者中P-gp的表达，miR-145-5p的表达变化对MDR1 mRNA及P-gp的影响及两者与临床病理特征之间的相关性。  结果 大肠癌组织中miR-145-5p表达量明显低于癌旁正常组织，MDR1 mRNA及P-gp的表达量明显高于癌旁正常组织(r=-0.403，P<0.01)。大肠癌细胞(HCT-15)中miR-145-5p 能够抑制MDR1 mRNA及P-gp的表达(P<0.05)。  结论 MiR-145-5p对大肠癌多药耐药基因及蛋白的表达具有调控作用，参与了大肠癌的发生、发展及多药耐药。

关键词: 大肠癌；MiR-145-5p, P-糖蛋白, 多药耐药, 免疫组织化学, 实时定量聚合酶连反应, 免疫印迹法, 人

Abstract:

Objective To investigate the expression and relationship between miR-145-5p and multidrug resistance gene (MDR1) protein P-glycoprotein(P-gp) in colorectal cancer.  Methods SP, Real-time PCR and Western blotting were used to detect the expression of P-gp colorectal carcinoma (CRC) in 50 patients and in 30 paracancerous normal tissue patients, the effect of miR-145-5p expression on MDR1 mRNA and P-gp and the correlation between them and clinicopathological features.   Results The expression level of miR-145-5p in CRC was significantly lower than that in paracancerous normal tissue, and the expression level of MDR1 mRNA and P-gp was significantly higher than that in paracancerous normal tissue (r=-0.403, P<0.01).MiR-145-5p could inhibit the expression of MDR1 mRNA and P-gp in HCT-15 cells(P<0.05).   Conclusion MiR-145-5p plays a regulatory role in the expression of multidrug resistance genes and proteins in colorectal cancer, and is involved in the occurrence, development and multidrug resistance of colorectal cancer.

Key words: Colorectal carcinoma, MiR-145-5p, P-glycoprotein, Multidrug resistance, Immunohistochemistry, Real-time PCR, Western blotting, Human

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