中等强度运动对去卵巢大鼠骨质疏松及骨形态发生蛋白2信号通路的影响

doi:10.16098/j.issn.0529-1356.2020.06.020

摘要/Abstract

摘要：

目的探讨中等强度运动对去卵巢大鼠骨密度、骨质代谢、骨生物力学及骨形态发生蛋白2(BMP-2)信号通路的影响。方法将24只成年雌性SD大鼠随机分为假手术组、手术组与运动组，每组8只，假手术组仅切除双侧卵巢周围脂肪组织，手术组、运动组摘除双侧卵巢，摘除卵巢1周后运动组进行中等强度运动训练，共训练12周。12周后，进行股骨骨密度、生物力学、骨代谢、组织学检测，Western blotting检测股骨组织BMP-2、Runt相关转录因子2（Runx2）、Osterix及Smad1/5/8蛋白表达。结果手术组、运动组血钙、血磷、血抗酒石酸酸性磷酸酶(TRACP)浓度高于假手术组(P<0.05)，而运动组上述指标低于手术组(P<0.05)。手术组和运动组骨密度低于假手术组(P<0.05)，而运动组高于手术组(P<0.05)。手术组和运动组骨组织生物力学性能低于假手术组(P<0.05)，而运动组高于手术组(P<0.05)。组织学显示，运动组骨质疏松程度轻于手术组，骨组织内BMP-2表达量多于手术组。手术组和运动组BMP-2、Runx2、Osterix及Smad1/5/8蛋白表达低于假手术组(P<0.05)，而运动组上述蛋白表达高于手术组(P<0.05)。结论中等强度运动可改善去卵巢大鼠股骨的骨密度、生物力学性能与骨代谢，这一作用可能与BMP-2信号通路有关。

关键词: 骨质疏松, 中等强度运动训练, 骨密度, 骨质代谢, 骨生物力学, 骨形态发生蛋白2信号通路, 免疫印迹法, 大鼠

Abstract:

Objective To investigate the effects of moderate intensity exercise on bone mineral density, bone metabolism, bone biomechanics and bone morphogenetic protein 2(BMP-2) signal pathway in ovariectomized rats. Methods Twenty-four adult female SD rats were randomly divided into sham operation group, operation group and exercise group with 8 rats in each group. The sham operation group only had bilateral peri-ovarian adipose tissue excised, while the operation group and the exercise group had bilateral ovaries excised. One week after ovariectomy, the exercise group received moderate intensity exercise training for 12 weeks. After 12 weeks, bone mineral density（BMD）, biomechanics, bone metabolism and histology of femur were measured, and the expression of BMP-2, Runt-related transcription factor 2(Runx2), Osterix and SMAD1 / 5 / 8 in femur were detected by Western blotting. Results The levels of serum calcium, phosphorus and tartrate resistant acid phosphatase in operation group and exercise group were higher than those in sham operation group (P<0.05), while those in exercise group were lower than those in operation group (P<0.05).BMD in operation group and exercise group was lower than that in sham operation group (P<0.05), while that in exercise group was higher than that in operation group(P<0.05).The biomechanical properties of bone tissue in operation group and exercise group were lower than that in sham operation group(P<0.05), while that in exercise group was higher than that in operation group(P<0.05).Histology showed that the degree of osteoporosis in exercise group was less than that in operation group, and the expression of BMP-2 in bone tissue was more than that in operation group. The expression of BMP-2, Runx2, Osterix and SMAD1/5/8 in operation group and exercise group was lower than that in sham operation group (P<0.05) but the expression of these proteins in exercise group was higher than that in operation group. Conclusion Moderate intensity exercise can improve bone mineral density, biomechanical properties and bone metabolism of femur in ovariectomized rats, which may be related to BMP-2 signaling pathway.

Key words: Osteoporosis, Moderate intensity exercise training, Bone mineral density, Bone metabolism, Bone biomechanics, Bone morphogenetic protein 2 signaling pathway, Western blotting, Rat

中图分类号:

Q591.8

张帅军. 中等强度运动对去卵巢大鼠骨质疏松及骨形态发生蛋白2信号通路的影响[J]. 解剖学报, 2020, 51(6): 934-939.

ZHANG Shuai-jun. Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats[J]. Acta Anatomica Sinica, 2020, 51(6): 934-939.

参考文献

［1］ Eastell R, Szulc P. Use of bone turnover markers in postmenopausal osteoporosis［J］.Lancet Diabetes Endocrinol,2017,5(11):908-923.

［2］ Moreira LD, Oliveira ML, Lirani-Galv?o AP, et al. Physical exercise and osteoporosis: effects of different types of exercises on bone and physical function of postmenopausal women［J］.Arq Bras Endocrinol Metabol,2014,58(5):514-522.

［3］ Liu GF, Wang ZQ, Liu L, et al. A network meta-analysis on the short-term efficacy and adverse events of different anti-osteoporosis drugs for the treatment of postmenopausal osteoporosis［J］.J Cell Biochem, 2018,119(6):4469-4481.

［4］ Komm BS, Morgenstern D, Yamamoto AL, et al.The safety and tolerability profile of therapies for the prevention and treatment of osteoporosis in postmenopausal women［J］. Expert Rev Clin Pharmacol,2015,8(6):769-784.

［5］ Li L, Zhang JP, Luo YSh. Effect of treadmill exercise of moderate intensity on the structure of the femoral head spongy bone in ovariectomized rats［J］.Chinese Journal of Anatomy,2016,39(6):702-705. (in Chinese)

李梁,张剑平,罗永胜.中等强度跑台运动对去卵巢大鼠股骨头骨松质结构的影响［J］.解剖学杂志,2016,39(6):702-705.

［6］ Dong WT, Huang K, Song M, et al. Effect of Guben Zenggu Decoction on expressions of serum BALP and CaM, CaMK Ⅱ mRNA in NEI network of ovariectomized rats［J］.Journal of Sichuan University (Medical Science Edition),2019,50(1): 27-33. (in Chinese)

董万涛,黄凯,宋敏,等.固本增骨方对去卵巢骨质疏松模型大鼠血清BALP及NEI网络组织中CaM、CaMK Ⅱ mRNA表达的影响［J］.四川大学学报(医学版), 2019,50(1): 27-33.

［7］ Qu LM, Gong W. Experimental evaluation of cornu cervi pantotrichum intervention in ovariectomized rats with osteoporosis［J］.Laboratory Animal and Comparative Medicine,2019,39(1): 52-55. (in Chinese)

曲雷鸣, 龚伟. 鹿茸干预去卵巢骨质疏松症模型大鼠的实验评价［J］.实验动物与比较医学,2019,39(1): 52-55.

［8］ Chen XC, Li Y, Yu HR, et al. Differences of bone mineral density and change with age among female adults of minorities in Guangxi［J］.Acta Anatomica Sinica, 2018,49(5):683-687. (in Chinese)

陈兴才,李炎,玉洪荣,等.广西少数民族成年女性骨密度的差异及年龄变化特点［J］.解剖学报,2018,49(5):683-687.

［9］ Wang B, Shi Q. Effects of long-term aerobic exercise on endogenous hydrogen sulfide production in bone tissue of ovariectomized rats［J］.Chinese Journal of Gerontology,2018,38(11):2715-2717. (in Chinese)

王兵,石琼.长期有氧运动对去卵巢大鼠骨组织中内源性硫化氢生成的影响［J］.中国老年学杂志, 2018,38(11):2715-2717.

［10］ Huang L, Xu J, Guo H, et al. Quantitative study of the influence of swimming therapy on osteoporosis rat models based on synchrotron radiation computed tomogaphy［J］.J Synchrotron Radiat,2018,25(Pt 3):793-800.

［11］ Chen TD, Zeng S, Bian PD. Correlation between exercise, bone mineral density and bone metabolism in the elderly［J］.Chinese Journal of Gerontology,2019,39(5):1115-1117. (in Chinese)

陈彤丹,曾苏,边平达.老年人运动与骨密度及骨代谢指标之间的相关性［J］.中国老年学杂志,2019,39(5):1115-1117.

［12］ Li Zh, Liu ShY, Ni GX, et al. Effects of different intensities of treadmill running on bone composition in rats［J］.Chinese Journal of Orthopaedic Trauma,2015,17(4):322-325．(in Chinese)

李哲,刘圣曜,倪国新,等.不同强度跑台运动对大鼠骨成分变化的影响［J］.中华创伤骨科杂志,2015,17(4):322-325．

［13］ Anand V, Vignesh U, Mehrotra D, et al. Evaluation of bone formation using recombinant human bone morphogenetic proteins-7 in small maxillofacial bony defects［J］.J Oral Maxillofac Pathol,2019,23(2):208-212.［14］ Lin H, Tang Y, Lozito TP, et al. Efficient in vivo bone formation by BMP-2 engineered human mesenchymal stem cells encapsulated in a projection stereolithographically fabricated hydrogel scaffold［J］.Stem Cell Res Ther, 2019,10(1):254.

［15］ Wang Z, Bao HW. Cnidium lactone stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via BMP-2/smad-signaling cascades mediated by estrogen receptor［J］. Am J Transl Res, 2019,11(8):4984-4991.

［16］ Cheng W, Yang S, Liang F, et al. Low-dose exposure to triclosan disrupted osteogenic differentiation of mouse embryonic stem cells via BMP/ERK/Smad/Runx-2 signalling pathway［J］. Food Chem Toxicol,2019,127:1-10.

[1]	洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉 . 酒精性肝纤维化核因子κB信号通路与性别的差异 [J]. 解剖学报, 2024, 55(1): 55-61.
[2]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[3]	袁蕾杨智伟杨惠刘政海何洁万炜. 三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛 [J]. 解剖学报, 2024, 55(1): 25-31.
[4]	马婷婷陈建红刘爱翠李海宁. 抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用 [J]. 解剖学报, 2024, 55(1): 32-42.
[5]	邹成功冯浩陈兵唐辉邵川孙谋杨荣何家全. 创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化 [J]. 解剖学报, 2024, 55(1): 43-48.
[6]	吕海燕沈西雅赵富生朱梅. 松茸多糖对 1-甲基-4-苯基-吡啶离子诱导 PC12 细胞损伤的影响 [J]. 解剖学报, 2024, 55(1): 49-54.
[7]	郑丽平刘霞杜晓华吴亚娟刘珊珊 . 脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布 [J]. 解剖学报, 2024, 55(1): 10-16.
[8]	孙雷王星宇谢水华. 老年骨质疏松性胸腰椎压缩性骨折患者PKP术后再发骨折的风险分析及列线图预测模型的构建[J]. 解剖学报, 2024, 55(1): 98-104.
[9]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[10]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.
[11]	王文娟丁雨桐苏昊任捷孙艳荣王涵菲陆嘉莉张林倩白钰秦丽华. 低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化[J]. 解剖学报, 2023, 54(6): 620-627.
[12]	张琴杨俊霞蒋青松. 福辛普利对糖尿病视网膜病变小鼠血管紧张素转化酶2和血管内皮生长因子的影响[J]. 解剖学报, 2023, 54(6): 628-634.
[13]	梁盼盼禹爱梅杜静寇文辉王欢欢宋爱霞. 基于c-Jun氨基末端激酶/p38丝裂原活化蛋白激酶信号通路探讨阿魏酸钠对偏头痛大鼠炎症反应的抑制作用[J]. 解剖学报, 2023, 54(6): 652-659.
[14]	徐颖刘斌郑兴何宗钊. 补体C3a受体在盲肠结扎穿孔致脓毒症大鼠认知障碍中的作用及机制 [J]. 解剖学报, 2023, 54(6): 668-675.
[15]	刘哲川李坤马帅男孟家琪王艳芹. 利拉鲁肽对百草枯诱导的小鼠帕金森病模型炎症及线粒体融合/分裂的影响 [J]. 解剖学报, 2023, 54(6): 676-681.