解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (1): 130-134.doi: 10.16098/j.issn.0529-1356.2021.01.021

• 组织学胚胎学发育生物学 • 上一篇    下一篇

微小RNA144-3p靶向作用于组蛋白去乙酰化酶2促进心肌细胞肥厚及心功能损伤

谭含璇1 彭绍蓉1 黄蔡华1 姜红峰1 刘娟2 龚芳2*   

  1. 1. 武汉市第四医院西院综合科（华中科技大学同济医学院附属普爱医院），武汉430033; 2. 湖北文理学院附属医院，襄阳市中心医院心内科，湖北 襄阳441021
  • 收稿日期:2019-08-26 修回日期:2019-11-04 出版日期:2021-02-06 发布日期:2021-02-06
  • 通讯作者: 龚芳 E-mail:1579355892@qq.com
  • 基金资助:
    2016年湖北省自然科学基金

MicroRNA144-3p targeting histone deacetylase 2 promoting cardiomyocyte hypertrophy and cardiac function damage

TAN Han-xuan1  PENG Shao-rong1  HUANG Cai-hua1  JIANG Hong-feng1  LIU Juan2  GONG Fang2*   

  1. 1.Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan430033,China； 2.Department of Cardiology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Hubei Xiangyang441021,China
  • Received:2019-08-26 Revised:2019-11-04 Online:2021-02-06 Published:2021-02-06
  • Contact: GONG Fang E-mail:1579355892@qq.com

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摘要：

目的  探讨微小RNA（miR）144-3p促进心肌细胞肥厚及心功能损伤的作用及可能的机制。  方法  将45只C57BL/6小鼠随机分为对照组、心肌肥厚模型组及miR144-3p转染组，采用超声心动图检测3组小鼠心功能相关指标；实验结束后，处死小鼠，对心肌组织进行HE染色；采用Real-time PCR技术，检测各组小鼠心肌细胞中miR144-3p的表达差异；采用Western blotting检测心肌组织中脑钠肽（BNP）蛋白、抗核因子（ANF）蛋白、β-肌球蛋白重链（β-MHC）、肌动蛋白α1（Acta1）蛋白、组蛋白去乙酰化酶2（HDAC2）蛋白的表达水平。  结果  与对照组相比，模型组和转染组的左室射血分数（EF）、舒张期室间隔厚度（IVSd）、收缩期室间隔厚度（IVSs）、舒张期左室后壁厚度（IVPWd）、收缩期左室后壁厚度（IVPWs）、心重指数、左心指数明显较高，而收缩期左室内径（LVDs）、舒张期左室内径（LVDd）较低（P＜0.05），但模型组、转染组之间各项指标差异不明显（P＞0.05）。与对照组相比，模型组和转染组miR144-3p相对表达量明显升高，且转染组明显高于模型组（P＜0.05）。与对照组相比，模型组和转染组ANF、β-MHC、Acta1和HDAC2蛋白表达水平明显增高（P＜0.05）。  结论  MiR144-3p可能通过上调HDAC2加重心肌肥厚及心功能损伤。

关键词: 心肌肥厚, 微小RNA144-3p, 组蛋白去乙酰化酶2, 腹主动脉缩窄, 超声心动图, 实时定量聚合酶链反应, 免疫印迹法, 小鼠

Abstract:

Objective  To investigate the role and possible mechanism of microRNA(miR)144-3p in promoting cardiomyocyte hypertrophy.   Methods  Forty-five C57BL/6 mice were divided into control group, myocardial hypertrophy model group (model group), and miR144-3p transfection group (transfection group) according to their transfection method. The cardiac function related indexes of three groups of mice were detected. HE staining was performed on mouse myocardial tissue.The expression of miR144-3p in mouse cardiomyocytes was detected by Real-time PCR. Antinuclear factor (ANF), β-myosin heavy chain (β-MHC), actin α1(Acta1) and histone deacetylase 2 (HDAC2) were detected by Western blotting in three groups.    Results  Compared with the control group, the interventricular septal thickness- diastolic（IVSd）, interventricular septal thickness-systolic（IVSs）, diastolic left ventricular posterior wall thickness（IVPWd）, systolic left ventricular posterior wall thickness（IVPWs）, ejection fraction（EF）, cardiac weight index and left cardiac index of the model group and the transfection group were significantly higher, while systolic left ventricular diameter（LVDs）and diastolic left ventricular diameter（LVDd）were lower (P<0.05), but there was no significant difference between the model group and the transfection group(P>0.05). Compared with the control group, the relative expression of miR144-3p in the model group and the transfection group was significantly higher than that in the model group (P<0.05). Compared with the control group, the expression levels of antinuclear factor, β-myosin heavy chain, Actinα1 and histone deacetylase 2 in the model group and the transfected group were significantly higher (P<0.05).    Conclusion  miR144-3p can aggravate cardiac hypertrophy by up-regulating HDAC2 and is expected to become a new therapeutic target.

Key words: Cardiac hypertrophy, MicroRNA144-3p, Histone deacetylase 2, Abdominal aortic constriciton, Echocardiography, Real-time PCR, Western blotting, Mouse

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