小鼠心肌肥厚枢纽基因的筛选与鉴定

doi:10.16098/j.issn.0529-1356.2021.03.010

解剖学报 ›› 2021, Vol. 52 ›› Issue (3): 391-397.doi: 10.16098/j.issn.0529-1356.2021.03.010

小鼠心肌肥厚枢纽基因的筛选与鉴定

史卓^1,2,3 李家慧⁴高静⁵董艳莉⁶任甫^1,2* 文普帅^2,7*

1.锦州医科大学基础医学院解剖学教研室，辽宁锦州 121000; 2.锦州医科大学辽宁省人类表型组重点研究实验室，辽宁锦州 121000; 3.锦州市卫生学校护理教研室，辽宁锦州 121000; 4.锦州医科大学第三临床医学院，辽宁锦州 121000; 5.锦州医科大学附属第一医院超声科，辽宁锦州 121000; 6.朝阳市卫生学校实验中心护理组，辽宁朝阳 122000;7.锦州医科大学基础医学院病理生理学教研室，辽宁锦州 121000

收稿日期:2020-07-14 修回日期:2020-09-04 出版日期:2021-06-06 发布日期:2021-06-06
通讯作者: 任甫;文普帅 E-mail:rf@symc.edu.cn
基金资助:
辽宁省科技厅自然科学基金项目;辽宁省大学生创新创业训练计划项目

Screening and identification the hub genes of cardiac hypertrophy in mice

SHI Zhuo^1,2,3 LI Jia-hui⁴ GAO Jing⁵ DONG Yan-li⁶ REN Fu^1,2* WEN Pu-shuai^2,7*

1.Department of Anatomy, School of Basic Medical Science, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 2.Liaoning Key Research Laboratory of Human Phenotype Group, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 3.Department of Nursing, Jinzhou Health School, Liaoning Jinzhou 121000, China; 4.The Third Clinical Medical College, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 5.Department of Ultrasound, the First Affiliated Hospital of Jinzhou Medical University, Liaoning Jinzhou 121000, China; 6.Nursing Group of Experimental Center, Chaoyang Health School, Liaoning Chaoyang 122000, China; 7.Department of Pathophysiology, School of Basic Medical Science, Jinzhou Medical University, Liaoning Jinzhou 121000, China

Received:2020-07-14 Revised:2020-09-04 Online:2021-06-06 Published:2021-06-06
Contact: REN Fu;WEN Pu-shuai E-mail:rf@symc.edu.cn
Supported by:
Supported by the Natural Science Foundation of Liaoning Provincial Department of Science and Technology

摘要/Abstract

摘要：

目的通过生物信息学分析结合生物学实验，筛选和鉴定与心肌肥厚密切相关的枢纽基因。方法从基因表达数据库(GEO)下载小鼠心肌肥厚相关芯片数据，利用GEO2R在线工具筛选差异表达基因；利用DAVID 6.7、String 11.0和Cytoscape 3.7.0软件对差异基因进行分析；昆明小鼠随机分为生理盐水组（n=6）和血管紧张素Ⅱ（Ang Ⅱ）组（n=6），建立心肌肥厚模型，通过Real-time PCR方法检测枢纽基因在Ang Ⅱ诱导的昆明小鼠心肌肥厚模型中的表达。结果筛选出共有差异表达基因202个，枢纽基因12个；Real-time PCR结果显示核心蛋白聚糖（Dcn）、HADHA和热休克蛋白(HSP)90αA1在AngⅡ组中表达明显下调。结论筛选出的枢纽基因可以通过细胞外基质和转化生长因子β(TGF-β)影响昆明小鼠心肌肥厚的发生发展。

关键词: 心肌肥厚, 生物信息学, 差异表达基因, 实时定量聚合酶链反应, 昆明小鼠

Abstract:

Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments. Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus(GEO) database, and the GEO2R online tool was adopted to screen for differentially expressed genes; DAVID 6.7, String 11.0 and Cytoscape 3.7.0 softwares were used to analyze differentially expressed genes; Kunming mice were randomly divided into a normal saline group (n=6) and an angiotensin Ⅱ (Ang Ⅱ) group (n=6) to establish a cardiac hypertrophy model, the expression of hub genes in Kunming mouse model of cardiac hypertrophy induced by AngⅡ was detected by Real-time PCR method . Results A total of 202 common differentially expressed genes and 12 hub genes were selected; the Real-time PCR result demonstrated that decorin(Dcn), HADHA and heat shock protein(HSP)90αA1 were significantly down-regulated in the AngⅡ group. Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).

Key words: Cardiac hypertrophy, Bioinformatics, Differentially expressed gene, Real-time PCR, Kunming mouse

中图分类号:

R361

史卓李家慧高静董艳莉任甫文普帅 . 小鼠心肌肥厚枢纽基因的筛选与鉴定[J]. 解剖学报, 2021, 52(3): 391-397.

SHI Zhuo LI Jia-hui GAO Jing DONG Yan-li REN Fu WEN Pu-shuai. Screening and identification the hub genes of cardiac hypertrophy in mice[J]. Acta Anatomica Sinica, 2021, 52(3): 391-397.

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小鼠心肌肥厚枢纽基因的筛选与鉴定

Screening and identification the hub genes of cardiac hypertrophy in mice

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